Restriction map for
pFN21AE5257
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DpnII DraI DraII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI Sau3AI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 186 CGCG CG^CG
AcsI 433 GAATTT [GA]^AATT[CT]
AhdI 127 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 433 GAATTT [GA]^AATT[CT]
AspEI 127 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 551 GAGCTC G[AT]GC[AT]^C
AvaII 299 GGACC G^G[AT]CC
BanII 551 GAGCTC G[GA]GC[CT]^C
BbsI 90 GTCTTC GAAGAC(2/6)
BbvCI 29 CCTCAGC CCTCAGC(-5/-2)
BcnI 49 CCCGG CC^[GC]GG
BmyI 551 GAGCTC G[AGT]GC[ACT]^C
BpuAI 90 GTCTTC GAAGAC(2/6)
BsiHKAI 551 GAGCTC G[AT]GC[AT]^C
Bsp1286I 551 GAGCTC G[AGT]GC[ACT]^C
BsrI 503 ACTGG ACTGG(1/-1)
BstF5I 327 CATCC GGATG(2/0)
BstNI 330 CCTGG CC^[AT]GG
BstUI 186 CGCG CG^CG
BstXI 111 CCAAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 185 GCGC GCG^C
Cfr13I 299 GGACC G^G[ACGT]CC
DraIII 20 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 127 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 153 CTCTTC CTCTTC(1/4)
EcoNI 117 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 330 CCTGG ^CC[AT]GG
EcoT22I 491 ATGCAT ATGCA^T
FokI 327 CATCC GGATG(9/13)
HaeIII 144 GGCC GG^CC
HapII 50 CCGG C^CGG
HhaI 185 GCGC GCG^C
HinP1I 185 GCGC G^CGC
HpaII 50 CCGG C^CGG
Ksp632I 153 CTCTTC CTCTTC(1/4)
MspI 50 CCGG C^CGG
MvaI 330 CCTGG CC^[AT]GG
MvnI 186 CGCG CG^CG
NciI 49 CCCGG CC^[GC]GG
NlaIV 298 GGGACC GG[ACGT]^[ACGT]CC
NsiI 491 ATGCAT ATGCA^T
NspI 195 GCATGT [GA]CATG^[CT]
SacI 551 GAGCTC GAGCT^C
Sau96I 299 GGACC G^G[ACGT]CC
ScaI 260 AGTACT AGT^ACT
SmlI 268 CTCAAG C^T[CT][GA]AG
Tsp509I 434 AATT ^AATT
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# TWO CUT ENZYMES ( 18 enzymes) #
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BciVI 11 GGATAC GTATCC(6/5)
478 GGATAC
BpmI 234 CTGGAG CTGGAG(16/14)
331 CTGGAG
BsaJI 117 CCTTGG C^C[ACGT][ACGT]GG
556 CCAAGG
BseRI 162 CTCCTC GAGGAG(10/8)
171 CTCCTC
BsiYI 117 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
556 CCAAGGAGAGG
BslI 117 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
556 CCAAGGAGAGG
BsmFI 298 GGGAC GGGAC(10/14)
97 GTCCC
BspMI 44 ACCTGC ACCTGC(4/8)
106 ACCTGC
BssKI 49 CCCGG ^CC[ACGT]GG
330 CCTGG
DdeI 30 CTCAG C^T[ACGT]AG
393 CTTAG
EcoT14I 117 CCTTGG C^C[AT][AT]GG
556 CCAAGG
FauI 187 GCGGG CCCGC(4/6)
296 GCGGG
MaeIII 103 GTCAC ^GT[ACGT]AC
135 GTCAC
MspA1I 231 CCGCTG C[AC]G^C[GT]G
294 CAGCGG
ScrFI 49 CCCGG CC^[ACGT]GG
330 CCTGG
SfaNI 326 GCATC GCATC(5/9)
493 GCATC
StyI 117 CCTTGG C^C[AT][AT]GG
556 CCAAGG
Tsp45I 103 GTCAC ^GT[GC]AC
135 GTCAC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AciI AfaI AluI BbvI Fnu4HI
HphI ItaI MboII MnlI MseI
MwoI NlaIII RsaI Tru9I TseI