Restriction map for
pFN21AE2645
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BclI BfrI
BglI BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I CpoI DraI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI Ksp632I KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 1219 GGTACC G^GTACC
Asp700I 1747 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1219 GGTACC G^GTACC
AvaI 1506 CCCGGG C^[CT]CG[GA]G
BanI 1219 GGTACC G^G[CT][GA]CC
BciVI 365 GGATAC GTATCC(6/5)
BsaBI 928 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1560 GGTCTC GGTCTC(1/5)
BsmI 298 GAATGC GAATGC(1/-1)
BsoBI 1506 CCCGGG C^[CT]CG[GA]G
BspDI 925 ATCGAT AT^CGAT
BspMI 1486 GCAGGT ACCTGC(4/8)
Cac8I 1315 GCTTGC GC[ACGT]^[ACGT]GC
ClaI 925 ATCGAT AT^CGAT
DraIII 159 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 796 CCATGG C^C[GA][CT]GG
EcoRI 1003 GAATTC G^AATTC
EcoRV 508 GATATC GAT^ATC
HindIII 1674 AAGCTT A^AGCTT
KpnI 1219 GGTACC GGTAC^C
MamI 928 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 796 CCATGG C^CATGG
PpuMI 237 AGGACCC [GA]G^G[AT]CC[CT]
SmaI 1506 CCCGGG CCC^GGG
SspI 822 AATATT AAT^ATT
XmaCI 1506 CCCGGG C^CCGGG
XmaI 1506 CCCGGG C^CCGGG
XmnI 1747 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 772 ACATGT A^C[GA][CT]GT
1749 ACATGT
AlwNI 13 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1757 CAGGTTCTG
AvrII 234 CCTAGG C^CTAGG
1128 CCTAGG
BcgI 1452 CGACTTTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1832 CGACTGCCTTGC
BglII 933 AGATCT A^GATCT
1263 AGATCT
BlnI 234 CCTAGG C^CTAGG
1128 CCTAGG
BlpI 1234 GCTCAGC GC^T[ACGT]AGC
1422 GCTAAGC
Bpu1102I 1234 GCTCAGC GC^T[ACGT]AGC
1422 GCTAAGC
BspHI 984 TCATGA T^CATGA
1308 TCATGA
BspLU11I 772 ACATGT A^CATGT
1749 ACATGT
CelII 1234 GCTCAGC GC^T[ACGT]AGC
1422 GCTAAGC
DraII 237 AGGACCC [GA]G^G[ACGT]CC[CT]
1814 AGGGCCT
EcoNI 1378 CCTACTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1735 CCTGGGACAGG
EcoO109I 237 AGGACCC [GA]G^G[ACGT]CC[CT]
1814 AGGGCCT
HapII 1507 CCGG C^CGG
1552 CCGG
HincII 21 GTTGAC GT[CT]^[GA]AC
1395 GTCAAC
HindII 21 GTTGAC GT[CT]^[GA]AC
1395 GTCAAC
HpaII 1507 CCGG C^CGG
1552 CCGG
MaeII 482 ACGT A^CGT
1155 ACGT
MslI 1009 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1629 CATCCCTGTG
MspI 1507 CCGG C^CGG
1552 CCGG
RcaI 984 TCATGA T^CATGA
1308 TCATGA
StuI 123 AGGCCT AGG^CCT
1732 AGGCCT
TaqI 926 TCGA T^CGA
1451 TCGA
Tsp45I 1103 GTGAC ^GT[GC]AC
1541 GTGAC
TthHB8I 926 TCGA T^CGA
1451 TCGA
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII