Restriction map for
pFN21AE4444
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BclI
BcnI BfrI BglI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BssHII Bst1107I
BstAPI BstBI BstZ17I CfoI ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 1261 GTCTAC GT^[AC][GT]AC
AcsI 640 GAATTC [GA]^AATT[CT]
Alw44I 1058 GTGCAC G^TGCAC
AocI 763 CCTCAGG CC^T[ACGT]AGG
ApaLI 1058 GTGCAC G^TGCAC
ApoI 640 GAATTC [GA]^AATT[CT]
BalI 119 TGGCCA TGG^CCA
BamHI 1047 GGATCC G^GATCC
BanI 571 GGTGCC G^G[CT][GA]CC
BbsI 623 GAAGAC GAAGAC(2/6)
BbvCI 225 CCTCAGC CCTCAGC(-5/-2)
BciVI 1174 GTATCC GTATCC(6/5)
BfaI 46 CTAG C^TAG
BlpI 180 GCTCAGC GC^T[ACGT]AGC
BpmI 613 CTGGAG CTGGAG(16/14)
Bpu1102I 180 GCTCAGC GC^T[ACGT]AGC
BpuAI 623 GAAGAC GAAGAC(2/6)
BsaAI 832 TACGTA [CT]AC^GT[GA]
BsaI 306 GAGACC GGTCTC(1/5)
BsmAI 306 GAGAC GTCTC(1/5)
BsmFI 1231 GGGAC GGGAC(10/14)
BsmI 743 GCATTC GAATGC(1/-1)
BspHI 332 TCATGA T^CATGA
BsrFI 284 GCCGGT [GA]^CCGG[CT]
BssSI 340 CTCGTG CACGAG(-5/-1)
BstEII 580 GGTTACC G^GT[ACGT]ACC
BstPI 580 GGTTACC G^GT[ACGT]ACC
BstXI 969 CCACCTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1047 GGATCC [GA]^GATC[CT]
Bsu36I 763 CCTCAGG CC^T[ACGT]AGG
CelII 180 GCTCAGC GC^T[ACGT]AGC
Cfr10I 284 GCCGGT [GA]^CCGG[CT]
DraIII 678 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 903 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 763 CCTCAGG CC^T[ACGT]AGG
EcoO65I 580 GGTTACC G^GT[ACGT]ACC
EcoRI 640 GAATTC G^AATTC
EcoT14I 400 CCAAGG C^C[AT][AT]GG
FauI 1043 GCGGG CCCGC(4/6)
KspI 1041 CCGCGG CCGC^GG
MaeI 46 CTAG C^TAG
MaeII 833 ACGT A^CGT
MflI 1047 GGATCC [GA]^GATC[CT]
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
MspA1I 1041 CCGCGG C[AC]G^C[GT]G
PpuMI 767 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 168 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 250 CTGCAG CTGCA^G
RcaI 332 TCATGA T^CATGA
SacII 1041 CCGCGG CCGC^GG
SapI 1248 GCTCTTC GCTCTTC(1/4)
SnaBI 832 TACGTA TAC^GTA
StyI 400 CCAAGG C^C[AT][AT]GG
TfiI 242 GATTC G^A[AT]TC
Tsp509I 641 AATT ^AATT
XhoII 1047 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 481 GGGCCC GGGCC^C
605 GGGCCC
BseRI 941 CTCCTC GAGGAG(10/8)
1094 GAGGAG
BsgI 702 GTGCAG GTGCAG(16/14)
1281 GTGCAG
Bsp1407I 206 TGTACA T^GTACA
1313 TGTACA
BsrDI 850 GCAATG GCAATG(2/0)
898 GCAATG
BsrGI 206 TGTACA T^GTACA
1313 TGTACA
DpnII 266 GATC ^GATC
1048 GATC
DraII 533 AGGGCCT [GA]G^G[ACGT]CC[CT]
767 AGGACCC
DsaI 257 CCGTGG C^C[GA][CT]GG
1041 CCGCGG
EcoO109I 533 AGGGCCT [GA]G^G[ACGT]CC[CT]
767 AGGACCC
HapII 285 CCGG C^CGG
303 CCGG
HpaII 285 CCGG C^CGG
303 CCGG
MboI 266 GATC ^GATC
1048 GATC
MslI 117 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
541 CACTACCATG
MspI 285 CCGG C^CGG
303 CCGG
NdeII 266 GATC ^GATC
1048 GATC
NspI 935 ACATGC [GA]CATG^[CT]
1001 ACATGC
Sau3AI 266 GATC ^GATC
1048 GATC
SspBI 206 TGTACA T^GTACA
1313 TGTACA
TaqI 812 TCGA T^CGA
1100 TCGA
TthHB8I 812 TCGA T^CGA
1100 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboII
MnlI MseI MvaI MvnI MwoI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp45I TspRI