Restriction map for
pFN21AE3805
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BpmI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MfeI MluI MroI MslI
MunI MwoI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuII RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 126 CGCG CG^CG
AcsI 328 AAATTC [GA]^AATT[CT]
AcyI 595 GACGCC G[GA]^CG[CT]C
ApaI 475 GGGCCC GGGCC^C
ApoI 328 AAATTC [GA]^AATT[CT]
Asp700I 69 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 524 TGGCCA TGG^CCA
BamHI 351 GGATCC G^GATCC
BanII 475 GGGCCC G[GA]GC[CT]^C
BcnI 48 CCCGG CC^[GC]GG
BglII 285 AGATCT A^GATCT
BlpI 28 GCTCAGC GC^T[ACGT]AGC
BmyI 475 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 28 GCTCAGC GC^T[ACGT]AGC
BsaHI 595 GACGCC G[GA]^CG[CT]C
BseRI 152 GAGGAG GAGGAG(10/8)
BsmBI 556 GAGACG CGTCTC(1/5)
Bsp1286I 475 GGGCCC G[AGT]GC[ACT]^C
BsrBI 479 CCGCTC CCGCTC(-3/-3)
BsrFI 102 GCCGGC [GA]^CCGG[CT]
BsrI 348 ACTGG ACTGG(1/-1)
BstNI 599 CCAGG CC^[AT]GG
BstUI 126 CGCG CG^CG
CelII 28 GCTCAGC GC^T[ACGT]AGC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
DraII 415 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 104 CGGCCG C^GGCCG
EclXI 104 CGGCCG C^GGCCG
Eco52I 104 CGGCCG C^GGCCG
EcoNI 315 CCTGCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 415 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 599 CCAGG ^CC[AT]GG
HgaI 595 GACGC GACGC(5/10)
Hin1I 595 GACGCC G[GA]^CG[CT]C
KspI 125 CCGCGG CCGC^GG
MaeIII 257 GTCAC ^GT[ACGT]AC
MluNI 524 TGGCCA TGG^CCA
MscI 524 TGGCCA TGG^CCA
MseI 280 TTAA T^TAA
MspA1I 125 CCGCGG C[AC]G^C[GT]G
MvaI 599 CCAGG CC^[AT]GG
MvnI 126 CGCG CG^CG
NaeI 102 GCCGGC GCC^GGC
NciI 48 CCCGG CC^[GC]GG
NgoMIV 102 GCCGGC G^CCGGC
NspI 404 GCATGC [GA]CATG^[CT]
PpuMI 415 GGGTCCT [GA]G^G[AT]CC[CT]
PvuI 462 CGATCG CGAT^CG
SacII 125 CCGCGG CCGC^GG
SphI 404 GCATGC GCATG^C
StuI 216 AGGCCT AGG^CCT
TaqI 148 TCGA T^CGA
TfiI 562 GAATC G^A[AT]TC
Tru9I 280 TTAA T^TAA
Tsp45I 257 GTCAC ^GT[GC]AC
TthHB8I 148 TCGA T^CGA
XmnI 69 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 53 AGCT AG^CT
141 AGCT
Aor51HI 336 AGCGCT AGC^GCT
390 AGCGCT
AvaII 122 GGTCC G^G[AT]CC
416 GGTCC
BsiEI 104 CGGCCG CG[GA][CT]^CG
462 CGATCG
BsiYI 315 CCTGCCGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
516 CCGCTACTTGG
BslI 315 CCTGCCGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
516 CCGCTACTTGG
BsmAI 556 GAGAC GTCTC(1/5)
546 GTCTC
BssKI 48 CCCGG ^CC[ACGT]GG
599 CCAGG
DdeI 29 CTCAG C^T[ACGT]AG
537 CTCAG
DsaI 125 CCGCGG C^C[GA][CT]GG
191 CCACGG
EaeI 104 CGGCCG [CT]^GGCC[GA]
524 TGGCCA
Eco47III 336 AGCGCT AGC^GCT
390 AGCGCT
EcoT14I 80 CCAAGG C^C[AT][AT]GG
551 CCTTGG
HaeII 336 AGCGCT [GA]GCGC^[CT]
390 AGCGCT
HphI 77 TCACC GGTGA(8/7)
258 TCACC
ScrFI 48 CCCGG CC^[ACGT]GG
599 CCAGG
SfaNI 398 GATGC GCATC(5/9)
485 GCATC
SmlI 224 CTCAAG C^T[CT][GA]AG
289 CTTGAG
StyI 80 CCAAGG C^C[AT][AT]GG
551 CCTTGG
TspRI 253 CAGTG CA[GC]TG[ACGT][ACGT]^
426 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AlwI BbvI BsaJI BstF5I
BstYI Cac8I CfoI Cfr13I DpnII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboI MboII MflI MnlI
MspI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I TseI Tsp509I XhoII