Restriction map for
pFN21AE5372
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoT22I
FauI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NarI NciI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 675 CGCG CG^CG
AciI 127 CCGC CCGC(-3/-1)
AluI 371 AGCT AG^CT
Alw44I 662 GTGCAC G^TGCAC
AocI 465 CCTAAGG CC^T[ACGT]AGG
ApaLI 662 GTGCAC G^TGCAC
Asp700I 471 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 662 GTGCAC G[AT]GC[AT]^C
BbvI 641 GCAGC GCAGC(8/12)
BclI 104 TGATCA T^GATCA
BmyI 662 GTGCAC G[AGT]GC[ACT]^C
BsaBI 201 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 395 GGTCTC GGTCTC(1/5)
BseRI 874 CTCCTC GAGGAG(10/8)
BsiHKAI 662 GTGCAC G[AT]GC[AT]^C
Bsp1286I 662 GTGCAC G[AGT]GC[ACT]^C
BsrDI 557 CATTGC GCAATG(2/0)
BsrFI 637 GCCGGC [GA]^CCGG[CT]
BsrI 513 ACTGG ACTGG(1/-1)
BstUI 675 CGCG CG^CG
BstYI 132 GGATCT [GA]^GATC[CT]
Bsu36I 465 CCTAAGG CC^T[ACGT]AGG
CfoI 674 GCGC GCG^C
Cfr10I 637 GCCGGC [GA]^CCGG[CT]
DsaI 379 CCATGG C^C[GA][CT]GG
EaeI 635 TGGCCG [CT]^GGCC[GA]
Eco81I 465 CCTAAGG CC^T[ACGT]AGG
EcoRI 220 GAATTC G^AATTC
EcoRV 363 GATATC GAT^ATC
FbaI 104 TGATCA T^GATCA
Fnu4HI 641 GCAGC GC^[ACGT]GC
HaeIII 636 GGCC GG^CC
HapII 638 CCGG C^CGG
HgaI 326 GCGTC GACGC(5/10)
HhaI 674 GCGC GCG^C
HinP1I 674 GCGC G^CGC
HinfI 7 GAGTC G^A[ACGT]TC
HpaII 638 CCGG C^CGG
ItaI 641 GCAGC GC^[ACGT]GC
MaeII 191 ACGT A^CGT
MaeIII 332 GTCAC ^GT[ACGT]AC
MamI 201 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 132 GGATCT [GA]^GATC[CT]
MspA1I 127 CCGCTG C[AC]G^C[GT]G
MspI 638 CCGG C^CGG
MvnI 675 CGCG CG^CG
NaeI 637 GCCGGC GCC^GGC
NcoI 379 CCATGG C^CATGG
NgoMIV 637 GCCGGC G^CCGGC
PleI 7 GAGTC GAGTC(4/5)
PstI 172 CTGCAG CTGCA^G
SfaNI 74 GCATC GCATC(5/9)
SfcI 172 CTGCAG C^T[GA][CT]AG
TseI 641 GCAGC G^C[AT]GC
Tsp45I 332 GTCAC ^GT[GC]AC
XbaI 160 TCTAGA T^CTAGA
XcmI 337 CCACTCTTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 132 GGATCT [GA]^GATC[CT]
XmnI 471 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaII 375 GGTCC G^G[AT]CC
453 GGTCC
BfaI 4 CTAG C^TAG
161 CTAG
BsiYI 638 CCGGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
652 CCTGGCAGGGG
BslI 638 CCGGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
652 CCTGGCAGGGG
BsmAI 9 GTCTC GTCTC(1/5)
396 GTCTC
BsmFI 376 GTCCC GGGAC(10/14)
454 GTCCC
BstF5I 786 CATCC GGATG(2/0)
383 GGATG
Cfr13I 375 GGTCC G^G[ACGT]CC
453 GGTCC
EarI 340 CTCTTC CTCTTC(1/4)
741 CTCTTC
EcoT14I 314 CCAAGG C^C[AT][AT]GG
379 CCATGG
FokI 786 CATCC GGATG(9/13)
383 GGATG
Ksp632I 340 CTCTTC CTCTTC(1/4)
741 CTCTTC
MaeI 4 CTAG C^TAG
161 CTAG
MseI 194 TTAA T^TAA
829 TTAA
Sau96I 375 GGTCC G^G[ACGT]CC
453 GGTCC
StyI 314 CCAAGG C^C[AT][AT]GG
379 CCATGG
Tru9I 194 TTAA T^TAA
829 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AlwI ApoI BsaJI BssKI
BstNI Cac8I DdeI DpnII EcoRII
HphI MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI Tsp509I TspRI