################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 1081 GCGG CCGC(-3/-1) AseI 6 ATTAAT AT^TAAT AsnI 6 ATTAAT AT^TAAT Asp700I 1141 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 59 GAGCTC G[AT]GC[AT]^C BanI 1034 GGCACC G^G[CT][GA]CC BcgI 956 GCAAATATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 756 CCCGG CC^[GC]GG BsaBI 430 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1585 GAGACC GGTCTC(1/5) BsgI 682 CTGCAC GTGCAG(16/14) BsiHKAI 59 GAGCTC G[AT]GC[AT]^C BsmBI 619 CGTCTC CGTCTC(1/5) BsmFI 976 GTCCC GGGAC(10/14) BspHI 1893 TCATGA T^CATGA BstBI 198 TTCGAA TT^CGAA DraIII 224 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 1756 GATATC GAT^ATC EcoT14I 127 CCTTGG C^C[AT][AT]GG EcoT22I 52 ATGCAT ATGCA^T HaeIII 771 GGCC GG^CC HapII 757 CCGG C^CGG HgaI 117 GCGTC GACGC(5/10) HindIII 479 AAGCTT A^AGCTT HpaII 757 CCGG C^CGG MamI 430 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 514 CAGCTG C[AC]G^C[GT]G MspI 757 CCGG C^CGG NciI 756 CCCGG CC^[GC]GG NsiI 52 ATGCAT ATGCA^T NspV 198 TTCGAA TT^CGAA PflMI 223 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 6 ATTAAT AT^TAAT PvuII 514 CAGCTG CAG^CTG RcaI 1893 TCATGA T^CATGA SacI 59 GAGCTC GAGCT^C ScaI 1127 AGTACT AGT^ACT SexAI 596 ACCTGGT A^CC[AT]GGT SfuI 198 TTCGAA TT^CGAA SpeI 526 ACTAGT A^CTAGT StyI 127 CCTTGG C^C[AT][AT]GG TaqI 199 TCGA T^CGA TthHB8I 199 TCGA T^CGA Van91I 223 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1141 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 407 GAATTT [GA]^AATT[CT] 1204 AAATTT AfaI 797 GTAC GT^AC 1128 GTAC ApoI 407 GAATTT [GA]^AATT[CT] 1204 AAATTT BamHI 570 GGATCC G^GATCC 1098 GGATCC BanII 59 GAGCTC G[GA]GC[CT]^C 505 GAGCCC BclI 435 TGATCA T^GATCA 918 TGATCA BpmI 460 CTGGAG CTGGAG(16/14) 1582 CTGGAG BseRI 289 GAGGAG GAGGAG(10/8) 994 GAGGAG BsiYI 223 CCACTCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1089 CCTCACGTTGG BslI 223 CCACTCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1089 CCTCACGTTGG Cac8I 546 GCAAGC GC[ACGT]^[ACGT]GC 789 GCAGGC DraI 72 TTTAAA TTT^AAA 828 TTTAAA FbaI 435 TGATCA T^GATCA 918 TGATCA HincII 1387 GTCAAC GT[CT]^[GA]AC 1674 GTTGAC HindII 1387 GTCAAC GT[CT]^[GA]AC 1674 GTTGAC PstI 1113 CTGCAG CTGCA^G 1131 CTGCAG RsaI 797 GTAC GT^AC 1128 GTAC SmlI 15 CTTGAG C^T[CT][GA]AG 245 CTCAAG SspI 1404 AATATT AAT^ATT 1934 AATATT Tsp45I 535 GTGAC ^GT[GC]AC 700 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AluI AlwI AvaII BbvI BfaI BmyI BsaJI BsmAI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XhoII