Restriction map for
pFN21AE3267
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 1081 GCGG CCGC(-3/-1)
AseI 6 ATTAAT AT^TAAT
AsnI 6 ATTAAT AT^TAAT
Asp700I 1141 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 59 GAGCTC G[AT]GC[AT]^C
BanI 1034 GGCACC G^G[CT][GA]CC
BcgI 956 GCAAATATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 756 CCCGG CC^[GC]GG
BsaBI 430 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1585 GAGACC GGTCTC(1/5)
BsgI 682 CTGCAC GTGCAG(16/14)
BsiHKAI 59 GAGCTC G[AT]GC[AT]^C
BsmBI 619 CGTCTC CGTCTC(1/5)
BsmFI 976 GTCCC GGGAC(10/14)
BspHI 1893 TCATGA T^CATGA
BstBI 198 TTCGAA TT^CGAA
DraIII 224 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1756 GATATC GAT^ATC
EcoT14I 127 CCTTGG C^C[AT][AT]GG
EcoT22I 52 ATGCAT ATGCA^T
HaeIII 771 GGCC GG^CC
HapII 757 CCGG C^CGG
HgaI 117 GCGTC GACGC(5/10)
HindIII 479 AAGCTT A^AGCTT
HpaII 757 CCGG C^CGG
MamI 430 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 514 CAGCTG C[AC]G^C[GT]G
MspI 757 CCGG C^CGG
NciI 756 CCCGG CC^[GC]GG
NsiI 52 ATGCAT ATGCA^T
NspV 198 TTCGAA TT^CGAA
PflMI 223 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 6 ATTAAT AT^TAAT
PvuII 514 CAGCTG CAG^CTG
RcaI 1893 TCATGA T^CATGA
SacI 59 GAGCTC GAGCT^C
ScaI 1127 AGTACT AGT^ACT
SexAI 596 ACCTGGT A^CC[AT]GGT
SfuI 198 TTCGAA TT^CGAA
SpeI 526 ACTAGT A^CTAGT
StyI 127 CCTTGG C^C[AT][AT]GG
TaqI 199 TCGA T^CGA
TthHB8I 199 TCGA T^CGA
Van91I 223 CCACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1141 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 407 GAATTT [GA]^AATT[CT]
1204 AAATTT
AfaI 797 GTAC GT^AC
1128 GTAC
ApoI 407 GAATTT [GA]^AATT[CT]
1204 AAATTT
BamHI 570 GGATCC G^GATCC
1098 GGATCC
BanII 59 GAGCTC G[GA]GC[CT]^C
505 GAGCCC
BclI 435 TGATCA T^GATCA
918 TGATCA
BpmI 460 CTGGAG CTGGAG(16/14)
1582 CTGGAG
BseRI 289 GAGGAG GAGGAG(10/8)
994 GAGGAG
BsiYI 223 CCACTCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1089 CCTCACGTTGG
BslI 223 CCACTCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1089 CCTCACGTTGG
Cac8I 546 GCAAGC GC[ACGT]^[ACGT]GC
789 GCAGGC
DraI 72 TTTAAA TTT^AAA
828 TTTAAA
FbaI 435 TGATCA T^GATCA
918 TGATCA
HincII 1387 GTCAAC GT[CT]^[GA]AC
1674 GTTGAC
HindII 1387 GTCAAC GT[CT]^[GA]AC
1674 GTTGAC
PstI 1113 CTGCAG CTGCA^G
1131 CTGCAG
RsaI 797 GTAC GT^AC
1128 GTAC
SmlI 15 CTTGAG C^T[CT][GA]AG
245 CTCAAG
SspI 1404 AATATT AAT^ATT
1934 AATATT
Tsp45I 535 GTGAC ^GT[GC]AC
700 GTGAC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AluI AlwI AvaII BbvI BfaI
BmyI BsaJI BsmAI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI XhoII