Restriction map for
pFN21AE6749
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI AspEI
AviII BamHI BbrPI BcgI BciVI
BfrI BglI BglII BsaAI BsaI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KspI MfeI
MflI MluI MroI MunI MvnI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 258 GGTACC G^GTACC
AflIII 1367 ACATGT A^C[GA][CT]GT
AgeI 595 ACCGGT A^CCGGT
Aor51HI 1022 AGCGCT AGC^GCT
Asp700I 402 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 258 GGTACC G^GTACC
AspI 1029 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1296 CCTAGG C^CTAGG
BbeI 736 GGCGCC GGCGC^C
BclI 1247 TGATCA T^GATCA
BlnI 1296 CCTAGG C^CTAGG
BsaBI 945 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1128 CTGCAC GTGCAG(16/14)
BsmI 539 GAATGC GAATGC(1/-1)
Bsp1407I 90 TGTACA T^GTACA
BspLU11I 1367 ACATGT A^CATGT
BsrBI 1405 CCGCTC CCGCTC(-3/-3)
BsrGI 90 TGTACA T^GTACA
BstAPI 195 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 499 CATCC GGATG(2/0)
DraIII 211 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 145 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1022 AGCGCT AGC^GCT
EcoNI 243 CCTTGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1247 TGATCA T^GATCA
FokI 499 CATCC GGATG(9/13)
HincII 301 GTTGAC GT[CT]^[GA]AC
HindII 301 GTTGAC GT[CT]^[GA]AC
KasI 736 GGCGCC G^GCGCC
KpnI 258 GGTACC GGTAC^C
MamI 945 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 741 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1183 GCCGGC GCC^GGC
NarI 736 GGCGCC GG^CGCC
NgoMIV 1183 GCCGGC G^CCGGC
PaeR7I 1410 CTCGAG C^TCGAG
PflFI 1029 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 595 ACCGGT A^CCGGT
PleI 1006 GAGTC GAGTC(4/5)
PshAI 324 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 415 CTGCAG CTGCA^G
PvuII 184 CAGCTG CAG^CTG
SacI 726 GAGCTC GAGCT^C
ScaI 835 AGTACT AGT^ACT
SfoI 736 GGCGCC GGC^GCC
SmlI 1410 CTCGAG C^T[CT][GA]AG
SspBI 90 TGTACA T^GTACA
SspI 368 AATATT AAT^ATT
Tth111I 1029 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1410 CTCGAG C^TCGAG
XmnI 402 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 553 GAATTT [GA]^AATT[CT]
576 AAATTT
AlwNI 184 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1051 CAGCTCCTG
ApoI 553 GAATTT [GA]^AATT[CT]
576 AAATTT
AspHI 726 GAGCTC G[AT]GC[AT]^C
778 GTGCTC
AvaI 1003 CCCGAG C^[CT]CG[GA]G
1410 CTCGAG
BfaI 134 CTAG C^TAG
1297 CTAG
BlpI 220 GCTGAGC GC^T[ACGT]AGC
780 GCTCAGC
BpmI 190 CTGGAG CTGGAG(16/14)
1569 CTGGAG
Bpu1102I 220 GCTGAGC GC^T[ACGT]AGC
780 GCTCAGC
BsaWI 595 ACCGGT [AT]^CCGG[AT]
881 ACCGGA
BsiHKAI 726 GAGCTC G[AT]GC[AT]^C
778 GTGCTC
BsmBI 322 GAGACG CGTCTC(1/5)
824 GAGACG
BsoBI 1003 CCCGAG C^[CT]CG[GA]G
1410 CTCGAG
BspHI 644 TCATGA T^CATGA
1250 TCATGA
BspMI 744 GCAGGT ACCTGC(4/8)
753 GCAGGT
BsrDI 374 CATTGC GCAATG(2/0)
974 GCAATG
CelII 220 GCTGAGC GC^T[ACGT]AGC
780 GCTCAGC
EarI 1077 CTCTTC CTCTTC(1/4)
32 GAAGAG
FauI 1404 CCCGC CCCGC(4/6)
662 GCGGG
Ksp632I 1077 CTCTTC CTCTTC(1/4)
32 GAAGAG
MaeI 134 CTAG C^TAG
1297 CTAG
NspI 970 ACATGC [GA]CATG^[CT]
1367 ACATGT
RcaI 644 TCATGA T^CATGA
1250 TCATGA
SexAI 146 ACCTGGT A^CC[AT]GGT
1270 ACCAGGT
Tsp45I 795 GTCAC ^GT[GC]AC
1103 GTGAC
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BmyI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I