Restriction map for
pFN21AE3064
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AlwI AlwNI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NlaIII NotI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 35 enzymes) #
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Aor51HI 533 AGCGCT AGC^GCT
Asp700I 375 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 289 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 54 CCCGGG C^[CT]CG[GA]G
BbeI 441 GGCGCC GGCGC^C
BbsI 295 GTCTTC GAAGAC(2/6)
BpuAI 295 GTCTTC GAAGAC(2/6)
BsoBI 54 CCCGGG C^[CT]CG[GA]G
BsrFI 112 GCCGGT [GA]^CCGG[CT]
BsrI 224 ACTGG ACTGG(1/-1)
BstAPI 239 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 131 CATCC GGATG(2/0)
Cfr10I 112 GCCGGT [GA]^CCGG[CT]
DraII 59 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 570 TGGCCG [CT]^GGCC[GA]
Eco47III 533 AGCGCT AGC^GCT
EcoNI 150 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 59 GGGACCC [GA]G^G[ACGT]CC[CT]
FokI 131 CATCC GGATG(9/13)
KasI 441 GGCGCC G^GCGCC
MboII 296 TCTTC GAAGA(8/7)
MslI 279 CACTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 441 GGCGCC GG^CGCC
NruI 411 TCGCGA TCG^CGA
PflFI 289 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 592 GAGTC GAGTC(4/5)
PpuMI 59 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 581 ACCTGGT A^CC[AT]GGT
SfoI 441 GGCGCC GGC^GCC
SmaI 54 CCCGGG CCC^GGG
StuI 158 AGGCCT AGG^CCT
Tth111I 289 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 54 CCCGGG C^CCGGG
XmaI 54 CCCGGG C^CCGGG
XmnI 375 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 412 CGCG CG^CG
574 CGCG
AciI 128 CCGC CCGC(-3/-1)
573 CCGC
Alw44I 178 GTGCAC G^TGCAC
418 GTGCAC
ApaLI 178 GTGCAC G^TGCAC
418 GTGCAC
BpmI 151 CTGGAG CTGGAG(16/14)
358 CTGGAG
BstUI 412 CGCG CG^CG
574 CGCG
DpnII 255 GATC ^GATC
306 GATC
HgaI 39 GACGC GACGC(5/10)
187 GACGC
MaeII 269 ACGT A^CGT
284 ACGT
MboI 255 GATC ^GATC
306 GATC
MvnI 412 CGCG CG^CG
574 CGCG
NdeII 255 GATC ^GATC
306 GATC
SacI 139 GAGCTC GAGCT^C
364 GAGCTC
Sau3AI 255 GATC ^GATC
306 GATC
SfaNI 210 GATGC GCATC(5/9)
130 GCATC
TfiI 100 GAATC G^A[AT]TC
380 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcyI AluI AspHI AvaII BanI
BanII BbvI BcnI BmyI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI TaqI
TseI Tsp45I TspRI TthHB8I