Restriction map for
pFN21AE4102
##################################
# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MluI MluNI MroI MscI
MslI MspA1I MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AciI 1148 GCGG CCGC(-3/-1)
Asp700I 1364 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1198 GGTCC G^G[AT]CC
AvrII 1017 CCTAGG C^CTAGG
BanII 445 GAGCCC G[GA]GC[CT]^C
BciVI 124 GTATCC GTATCC(6/5)
BlnI 1017 CCTAGG C^CTAGG
BsiYI 27 CCTCCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 27 CCTCCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 928 GTCTC GTCTC(1/5)
BsmFI 47 GTCCC GGGAC(10/14)
BsmI 279 GAATGC GAATGC(1/-1)
Bsp1407I 346 TGTACA T^GTACA
BsrGI 346 TGTACA T^GTACA
BstF5I 913 CATCC GGATG(2/0)
BstXI 50 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 1198 GGTCC G^G[ACGT]CC
DraII 1197 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1041 TGGCCG [CT]^GGCC[GA]
EcoNI 27 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1197 AGGTCCT [GA]G^G[ACGT]CC[CT]
FokI 913 CATCC GGATG(9/13)
HgaI 1192 GCGTC GACGC(5/10)
MfeI 1160 CAATTG C^AATTG
MunI 1160 CAATTG C^AATTG
NlaIV 200 GGAACC GG[ACGT]^[ACGT]CC
PleI 37 GAGTC GAGTC(4/5)
PpuMI 1197 AGGTCCT [GA]G^G[AT]CC[CT]
Sau96I 1198 GGTCC G^G[ACGT]CC
ScaI 62 AGTACT AGT^ACT
SspBI 346 TGTACA T^GTACA
SspI 845 AATATT AAT^ATT
XmnI 1364 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 425 AAATTC [GA]^AATT[CT]
549 AAATTC
AfaI 63 GTAC GT^AC
347 GTAC
AlwI 135 GGATC GGATC(4/5)
1128 GGATC
ApoI 425 AAATTC [GA]^AATT[CT]
549 AAATTC
BbsI 833 GTCTTC GAAGAC(2/6)
1109 GTCTTC
BbvI 497 GCAGC GCAGC(8/12)
406 GCTGC
BmyI 94 GGGCAC G[AGT]GC[ACT]^C
445 GAGCCC
BpmI 241 CTCCAG CTGGAG(16/14)
58 CTGGAG
BpuAI 833 GTCTTC GAAGAC(2/6)
1109 GTCTTC
BsgI 354 GTGCAG GTGCAG(16/14)
724 GTGCAG
Bsp1286I 94 GGGCAC G[AGT]GC[ACT]^C
445 GAGCCC
EcoT14I 988 CCAAGG C^C[AT][AT]GG
1017 CCTAGG
Fnu4HI 406 GCTGC GC^[ACGT]GC
497 GCAGC
HaeIII 1042 GGCC GG^CC
1346 GGCC
HphI 21 GGTGA GGTGA(8/7)
1270 GGTGA
ItaI 406 GCTGC GC^[ACGT]GC
497 GCAGC
MaeIII 10 GTGAC ^GT[ACGT]AC
79 GTGAC
MwoI 167 GCCGTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1125 GCAGGATCAGC
NlaIII 285 CATG CATG^
1173 CATG
RsaI 63 GTAC GT^AC
347 GTAC
SmlI 442 CTTGAG C^T[CT][GA]AG
450 CTTGAG
StyI 988 CCAAGG C^C[AT][AT]GG
1017 CCTAGG
TseI 406 GCTGC G^C[AT]GC
497 GCAGC
Tsp45I 10 GTGAC ^GT[GC]AC
79 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwNI BfaI BglII BsaJI
BseRI BsrI BssKI BstNI BstYI
DdeI DpnII EarI EcoRII HinfI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MvaI NdeII Sau3AI
ScrFI TfiI Tru9I Tsp509I TspRI
XbaI XhoII