Restriction map for
pFN21AE4050
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NlaIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 701 GTATAC GT^[AC][GT]AC
AflIII 189 ACATGT A^C[GA][CT]GT
Alw44I 119 GTGCAC G^TGCAC
AlwI 481 GATCC GGATC(4/5)
ApaLI 119 GTGCAC G^TGCAC
Asp700I 715 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 119 GTGCAC G[AT]GC[AT]^C
BbvI 93 GCAGC GCAGC(8/12)
BcgI 33 GCACTTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiHKAI 119 GTGCAC G[AT]GC[AT]^C
BspLU11I 189 ACATGT A^CATGT
BssKI 697 CCTGG ^CC[ACGT]GG
Bst1107I 701 GTATAC GTA^TAC
BstNI 697 CCTGG CC^[AT]GG
BstZ17I 701 GTATAC GTA^TAC
DdeI 254 CTGAG C^T[ACGT]AG
DpnII 481 GATC ^GATC
EcoRII 697 CCTGG ^CC[AT]GG
EcoT22I 279 ATGCAT ATGCA^T
Fnu4HI 93 GCAGC GC^[ACGT]GC
HaeIII 353 GGCC GG^CC
HphI 475 GGTGA GGTGA(8/7)
ItaI 93 GCAGC GC^[ACGT]GC
MboI 481 GATC ^GATC
MslI 282 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 319 CAGCTG C[AC]G^C[GT]G
MvaI 697 CCTGG CC^[AT]GG
MwoI 378 GCATGAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 356 CATATG CA^TATG
NdeII 481 GATC ^GATC
NsiI 279 ATGCAT ATGCA^T
NspI 189 ACATGT [GA]CATG^[CT]
PflMI 446 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 298 CTGCAG CTGCA^G
PvuII 319 CAGCTG CAG^CTG
SapI 382 GAAGAGC GCTCTTC(1/4)
Sau3AI 481 GATC ^GATC
ScrFI 697 CCTGG CC^[ACGT]GG
SfaNI 262 GATGC GCATC(5/9)
SpeI 553 ACTAGT A^CTAGT
TaqI 42 TCGA T^CGA
TseI 93 GCAGC G^C[AT]GC
TthHB8I 42 TCGA T^CGA
Van91I 446 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 715 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 144 AAATTC [GA]^AATT[CT]
751 GAATTT
ApoI 144 AAATTC [GA]^AATT[CT]
751 GAATTT
BfaI 437 CTAG C^TAG
554 CTAG
BmyI 31 GGGCAC G[AGT]GC[ACT]^C
119 GTGCAC
Bsp1286I 31 GGGCAC G[AGT]GC[ACT]^C
119 GTGCAC
BspHI 392 TCATGA T^CATGA
822 TCATGA
DraI 271 TTTAAA TTT^AAA
870 TTTAAA
EarI 382 GAAGAG CTCTTC(1/4)
657 GAAGAG
HindIII 397 AAGCTT A^AGCTT
665 AAGCTT
Ksp632I 382 GAAGAG CTCTTC(1/4)
657 GAAGAG
MaeI 437 CTAG C^TAG
554 CTAG
MnlI 345 GAGG CCTC(7/6)
500 GAGG
RcaI 392 TCATGA T^CATGA
822 TCATGA
SfcI 298 CTGCAG C^T[GA][CT]AG
373 CTACAG
SspI 99 AATATT AAT^ATT
244 AATATT
TspRI 451 CAGTG CA[GC]TG[ACGT][ACGT]^
540 CAGTG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AluI BsiYI BslI BsrI BstF5I
FokI HinfI MboII MseI NlaIII
TfiI Tru9I Tsp509I