Restriction map for
pFN21AE3071
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvI BciVI BclI
BcnI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmAI BsmBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrI BssHII BssKI BssSI
Bst1107I BstBI BstEII BstF5I BstNI
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HpaI HpaII HphI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TseI
Tth111I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 471 GGTACC G^GTACC
AcsI 493 AAATTT [GA]^AATT[CT]
AflII 595 CTTAAG C^TTAAG
AlwI 249 GATCC GGATC(4/5)
AocI 65 CCTCAGG CC^T[ACGT]AGG
ApoI 493 AAATTT [GA]^AATT[CT]
AseI 612 ATTAAT AT^TAAT
AsnI 612 ATTAAT AT^TAAT
Asp718I 471 GGTACC G^GTACC
AvaI 25 CTCGAG C^[CT]CG[GA]G
BanI 471 GGTACC G^G[CT][GA]CC
BbsI 331 GTCTTC GAAGAC(2/6)
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BcgI 31 GCAAGCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 595 CTTAAG C^TTAAG
BpuAI 331 GTCTTC GAAGAC(2/6)
BseRI 7 GAGGAG GAGGAG(10/8)
BsiEI 177 CGACCG CG[GA][CT]^CG
BsmFI 260 GGGAC GGGAC(10/14)
BsmI 391 GAATGC GAATGC(1/-1)
BsoBI 25 CTCGAG C^[CT]CG[GA]G
Bsp1407I 318 TGTACA T^GTACA
BsrDI 670 GCAATG GCAATG(2/0)
BsrGI 318 TGTACA T^GTACA
BstAPI 134 GCAAGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 476 CCACAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 248 AGATCC [GA]^GATC[CT]
Bsu36I 65 CCTCAGG CC^T[ACGT]AGG
Cfr13I 62 GGGCC G^G[ACGT]CC
DraII 61 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 424 CCACGG C^C[GA][CT]GG
EaeI 165 TGGCCG [CT]^GGCC[GA]
Eco81I 65 CCTCAGG CC^T[ACGT]AGG
EcoO109I 61 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 581 CCTTGG C^C[AT][AT]GG
Fnu4HI 167 GCCGC GC^[ACGT]GC
ItaI 167 GCCGC GC^[ACGT]GC
KpnI 471 GGTACC GGTAC^C
MaeIII 449 GTGAC ^GT[ACGT]AC
MflI 248 AGATCC [GA]^GATC[CT]
MspA1I 45 CAGCGG C[AC]G^C[GT]G
NheI 408 GCTAGC G^CTAGC
PaeR7I 25 CTCGAG C^TCGAG
PshBI 612 ATTAAT AT^TAAT
Sau96I 62 GGGCC G^G[ACGT]CC
SfcI 309 CTACAG C^T[GA][CT]AG
SspBI 318 TGTACA T^GTACA
StyI 581 CCTTGG C^C[AT][AT]GG
TaqI 26 TCGA T^CGA
Tsp45I 449 GTGAC ^GT[GC]AC
TspRI 53 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 26 TCGA T^CGA
XcmI 191 CCACGATAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 25 CTCGAG C^TCGAG
XhoII 248 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 319 GTAC GT^AC
472 GTAC
BfaI 359 CTAG C^TAG
409 CTAG
BsrBI 87 CCGCTC CCGCTC(-3/-3)
218 GAGCGG
CfoI 161 GCGC GCG^C
240 GCGC
DpnII 114 GATC ^GATC
249 GATC
HhaI 161 GCGC GCG^C
240 GCGC
HinP1I 161 GCGC G^CGC
240 GCGC
HindIII 33 AAGCTT A^AGCTT
592 AAGCTT
MaeI 359 CTAG C^TAG
409 CTAG
MaeII 529 ACGT A^CGT
676 ACGT
MboI 114 GATC ^GATC
249 GATC
MseI 596 TTAA T^TAA
613 TTAA
NdeII 114 GATC ^GATC
249 GATC
NlaIV 61 GGGGCC GG[ACGT]^[ACGT]CC
471 GGTACC
NspI 139 ACATGC [GA]CATG^[CT]
321 ACATGC
PleI 121 GACTC GAGTC(4/5)
16 GAGTC
RsaI 319 GTAC GT^AC
472 GTAC
Sau3AI 114 GATC ^GATC
249 GATC
SfaNI 95 GCATC GCATC(5/9)
242 GCATC
TfiI 281 GATTC G^A[AT]TC
429 GATTC
Tru9I 596 TTAA T^TAA
613 TTAA
Tsp509I 494 AATT ^AATT
642 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
Cac8I DdeI FauI HaeIII HinfI
MboII MnlI MwoI NlaIII SmlI