Restriction map for
pFN21AB7727
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco52I
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tru9I
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 1097 TCCGGA T^CCGGA
AcyI 163 GGCGCC G[GA]^CG[CT]C
AgeI 205 ACCGGT A^CCGGT
AhdI 1237 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1103 CCTCAGG CC^T[ACGT]AGG
Aor51HI 253 AGCGCT AGC^GCT
AspEI 1237 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 227 GTGCTC G[AT]GC[AT]^C
AvaI 159 CTCGGG C^[CT]CG[GA]G
BalI 794 TGGCCA TGG^CCA
BanI 163 GGCGCC G^G[CT][GA]CC
BanII 307 GAGCCC G[GA]GC[CT]^C
BbeI 163 GGCGCC GGCGC^C
BcgI 832 CGACAGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 148 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 163 GGCGCC G[GA]^CG[CT]C
BseAI 1097 TCCGGA T^CCGGA
BseRI 616 CTCCTC GAGGAG(10/8)
BsiHKAI 227 GTGCTC G[AT]GC[AT]^C
BsmI 337 GAATGC GAATGC(1/-1)
BsoBI 159 CTCGGG C^[CT]CG[GA]G
Bsp1407I 486 TGTACA T^GTACA
BspEI 1097 TCCGGA T^CCGGA
BspMI 920 ACCTGC ACCTGC(4/8)
BsrBI 880 GAGCGG CCGCTC(-3/-3)
BsrGI 486 TGTACA T^GTACA
BsrI 871 ACTGG ACTGG(1/-1)
Bsu36I 1103 CCTCAGG CC^T[ACGT]AGG
EaeI 794 TGGCCA [CT]^GGCC[GA]
Eam1105I 1237 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 991 GAAGAG CTCTTC(1/4)
Eco47III 253 AGCGCT AGC^GCT
Eco81I 1103 CCTCAGG CC^T[ACGT]AGG
EcoNI 576 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 46 GACGC GACGC(5/10)
Hin1I 163 GGCGCC G[GA]^CG[CT]C
HincII 742 GTCAAC GT[CT]^[GA]AC
HindII 742 GTCAAC GT[CT]^[GA]AC
KasI 163 GGCGCC G^GCGCC
Ksp632I 991 GAAGAG CTCTTC(1/4)
KspI 13 CCGCGG CCGC^GG
MaeII 643 ACGT A^CGT
MluNI 794 TGGCCA TGG^CCA
MroI 1097 TCCGGA T^CCGGA
MscI 794 TGGCCA TGG^CCA
MspA1I 13 CCGCGG C[AC]G^C[GT]G
NaeI 102 GCCGGC GCC^GGC
NarI 163 GGCGCC GG^CGCC
NcoI 197 CCATGG C^CATGG
NgoMIV 102 GCCGGC G^CCGGC
NspI 1149 ACATGC [GA]CATG^[CT]
PinAI 205 ACCGGT A^CCGGT
PstI 37 CTGCAG CTGCA^G
SacII 13 CCGCGG CCGC^GG
SexAI 788 ACCAGGT A^CC[AT]GGT
SfiI 147 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 163 GGCGCC GGC^GCC
SspBI 486 TGTACA T^GTACA
SspI 754 AATATT AAT^ATT
Tsp509I 1225 AATT ^AATT
XbaI 672 TCTAGA T^CTAGA
XcmI 1078 CCATACACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 623 GTAGAC GT^[AC][GT]AC
997 GTCTAC
AlwNI 805 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
916 CAGAACCTG
BsaWI 205 ACCGGT [AT]^CCGG[AT]
1097 TCCGGA
BsgI 352 CTGCAC GTGCAG(16/14)
1113 GTGCAG
BsmAI 530 GTCTC GTCTC(1/5)
1245 GTCTC
BsrDI 798 CATTGC GCAATG(2/0)
438 GCAATG
BsrFI 102 GCCGGC [GA]^CCGG[CT]
205 ACCGGT
BstEII 662 GGTCACC G^GT[ACGT]ACC
1211 GGTTACC
BstPI 662 GGTCACC G^GT[ACGT]ACC
1211 GGTTACC
BstXI 456 CCAAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
785 CCAACCAGGTGG
BstYI 390 GGATCT [GA]^GATC[CT]
1094 AGATCC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
205 ACCGGT
DsaI 13 CCGCGG C^C[GA][CT]GG
197 CCATGG
EcoO65I 662 GGTCACC G^GT[ACGT]ACC
1211 GGTTACC
EcoT14I 197 CCATGG C^C[AT][AT]GG
936 CCAAGG
MflI 390 GGATCT [GA]^GATC[CT]
1094 AGATCC
MslI 506 CACTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
685 CACAACAATG
PflMI 579 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
653 CCAAACCCTGG
PleI 218 GACTC GAGTC(4/5)
823 GAGTC
SfcI 37 CTGCAG C^T[GA][CT]AG
222 CTATAG
SmlI 331 CTTGAG C^T[CT][GA]AG
896 CTCAAG
StyI 197 CCATGG C^C[AT][AT]GG
936 CCAAGG
TaqI 888 TCGA T^CGA
1175 TCGA
TthHB8I 888 TCGA T^CGA
1175 TCGA
Van91I 579 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
653 CCAAACCCTGG
XhoII 390 GGATCT [GA]^GATC[CT]
1094 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI TseI Tsp45I TspRI