##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        Fnu4HI      FokI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       HphI        
ItaI        KasI        KpnI        KspI        MaeII       
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TseI        Tsp45I      TthHB8I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AlwI            5  GATCC                GGATC(4/5)
AlwNI         448  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          163  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          341  GAAGAC               GAAGAC(2/6)
BclI          138  TGATCA               T^GATCA
BpmI          188  CTCCAG               CTGGAG(16/14)
BpuAI         341  GAAGAC               GAAGAC(2/6)
BsaJI          87  CCAAGG               C^C[ACGT][ACGT]GG
BsmAI         202  GAGAC                GTCTC(1/5)
BsmI          113  GAATGC               GAATGC(1/-1)
BssKI          13  CCTGG                ^CC[ACGT]GG
BstNI          13  CCTGG                CC^[AT]GG
BstYI           4  AGATCC               [GA]^GATC[CT]
Cac8I         183  GCCAGC               GC[ACGT]^[ACGT]GC
EarI          309  GAAGAG               CTCTTC(1/4)
EcoRI         403  GAATTC               G^AATTC
EcoRII         13  CCTGG                ^CC[AT]GG
EcoT14I        87  CCAAGG               C^C[AT][AT]GG
FbaI          138  TGATCA               T^GATCA
HaeIII        243  GGCC                 GG^CC
HgaI          117  GCGTC                GACGC(5/10)
HincII        119  GTCAAC               GT[CT]^[GA]AC
HindII        119  GTCAAC               GT[CT]^[GA]AC
Ksp632I       309  GAAGAG               CTCTTC(1/4)
MflI            4  AGATCC               [GA]^GATC[CT]
MseI          299  TTAA                 T^TAA
MslI          469  CACGGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        142  CAGCTG               C[AC]G^C[GT]G
MvaI           13  CCTGG                CC^[AT]GG
NlaIV         168  GGTCCC               GG[ACGT]^[ACGT]CC
NspI          361  ACATGC               [GA]CATG^[CT]
PflFI         163  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI          63  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         142  CAGCTG               CAG^CTG
ScrFI          13  CCTGG                CC^[ACGT]GG
SfaNI         450  GCATC                GCATC(5/9)
StyI           87  CCAAGG               C^C[AT][AT]GG
Tru9I         299  TTAA                 T^TAA
TspRI          65  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       163  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          460  TCTAGA               T^CTAGA
XhoII           4  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AcsI          175  GAATTT               [GA]^AATT[CT]
              403  GAATTC              
ApoI          175  GAATTT               [GA]^AATT[CT]
              403  GAATTC              
AvaII          69  GGTCC                G^G[AT]CC
              168  GGTCC               
BfaI          409  CTAG                 C^TAG
              461  CTAG                
BsmFI          61  GGGAC                GGGAC(10/14)
              169  GTCCC               
BsrI           66  ACTGG                ACTGG(1/-1)
                8  CCAGT               
MaeI          409  CTAG                 C^TAG
              461  CTAG                


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BsaBI       Cfr13I      DpnII       HinfI       
MamI        MboI        MboII       MnlI        NdeII       
NlaIII      Sau3AI      Sau96I      TfiI        Tsp509I