Restriction map for
pFN21AE6581
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeIII HgaI Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MboII
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI StyI SwaI TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 25 GTAGAC GT^[AC][GT]AC
AlwI 380 GGATC GGATC(4/5)
Aor51HI 160 AGCGCT AGC^GCT
AvaII 242 GGACC G^G[AT]CC
BbvI 94 GCAGC GCAGC(8/12)
BpmI 246 CTGGAG CTGGAG(16/14)
BsaWI 29 ACCGGA [AT]^CCGG[AT]
BsmFI 241 GGGAC GGGAC(10/14)
BspHI 99 TCATGA T^CATGA
BssKI 245 CCTGG ^CC[ACGT]GG
BstBI 167 TTCGAA TT^CGAA
BstNI 245 CCTGG CC^[AT]GG
BstYI 380 GGATCT [GA]^GATC[CT]
Cac8I 90 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 161 GCGC GCG^C
Cfr13I 242 GGACC G^G[ACGT]CC
DdeI 235 CTCAG C^T[ACGT]AG
DpnII 381 GATC ^GATC
DraI 385 TTTAAA TTT^AAA
DraII 241 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 160 AGCGCT AGC^GCT
EcoO109I 241 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 245 CCTGG ^CC[AT]GG
Fnu4HI 94 GCAGC GC^[ACGT]GC
HaeII 160 AGCGCT [GA]GCGC^[CT]
HapII 30 CCGG C^CGG
HhaI 161 GCGC GCG^C
HinP1I 161 GCGC G^CGC
HindIII 88 AAGCTT A^AGCTT
HinfI 154 GAATC G^A[ACGT]TC
HpaII 30 CCGG C^CGG
ItaI 94 GCAGC GC^[ACGT]GC
MaeIII 199 GTCAC ^GT[ACGT]AC
MboI 381 GATC ^GATC
MflI 380 GGATCT [GA]^GATC[CT]
MnlI 256 GAGG CCTC(7/6)
MspI 30 CCGG C^CGG
MvaI 245 CCTGG CC^[AT]GG
NdeII 381 GATC ^GATC
NspV 167 TTCGAA TT^CGAA
PpuMI 241 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 99 TCATGA T^CATGA
Sau3AI 381 GATC ^GATC
Sau96I 242 GGACC G^G[ACGT]CC
ScrFI 245 CCTGG CC^[ACGT]GG
SfuI 167 TTCGAA TT^CGAA
SmlI 253 CTTGAG C^T[CT][GA]AG
SpeI 339 ACTAGT A^CTAGT
TaqI 168 TCGA T^CGA
TfiI 154 GAATC G^A[AT]TC
TseI 94 GCAGC G^C[AT]GC
Tsp45I 199 GTCAC ^GT[GC]AC
TthHB8I 168 TCGA T^CGA
XhoII 380 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 7 enzymes) #
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AcsI 139 AAATTC [GA]^AATT[CT]
277 AAATTC
ApoI 139 AAATTC [GA]^AATT[CT]
277 AAATTC
BfaI 217 CTAG C^TAG
340 CTAG
HphI 331 GGTGA GGTGA(8/7)
200 TCACC
MaeI 217 CTAG C^TAG
340 CTAG
NlaIII 54 CATG CATG^
100 CATG
NlaIV 241 GGGACC GG[ACGT]^[ACGT]CC
248 GGAGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
AluI MseI Tru9I Tsp509I