Restriction map for
pFN21AE3871
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvaI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AluI 17 AGCT AG^CT
AspHI 3 GTGCTC G[AT]GC[AT]^C
AvaI 103 CCCGGG C^[CT]CG[GA]G
AvaII 89 GGACC G^G[AT]CC
BamHI 99 GGATCC G^GATCC
BbsI 160 GAAGAC GAAGAC(2/6)
BfaI 355 CTAG C^TAG
BlpI 5 GCTCAGC GC^T[ACGT]AGC
BmyI 3 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 5 GCTCAGC GC^T[ACGT]AGC
BpuAI 160 GAAGAC GAAGAC(2/6)
BsiHKAI 3 GTGCTC G[AT]GC[AT]^C
BsoBI 103 CCCGGG C^[CT]CG[GA]G
Bsp1286I 3 GTGCTC G[AGT]GC[ACT]^C
BsrBI 73 GAGCGG CCGCTC(-3/-3)
BsrFI 186 GCCGGT [GA]^CCGG[CT]
BstYI 99 GGATCC [GA]^GATC[CT]
CelII 5 GCTCAGC GC^T[ACGT]AGC
Cfr10I 186 GCCGGT [GA]^CCGG[CT]
Cfr13I 89 GGACC G^G[ACGT]CC
DdeI 6 CTCAG C^T[ACGT]AG
DpnII 100 GATC ^GATC
HaeIII 337 GGCC GG^CC
HgaI 61 GACGC GACGC(5/10)
HinfI 163 GACTC G^A[ACGT]TC
MaeI 355 CTAG C^TAG
MboI 100 GATC ^GATC
MboII 160 GAAGA GAAGA(8/7)
MflI 99 GGATCC [GA]^GATC[CT]
NdeII 100 GATC ^GATC
NlaIII 252 CATG CATG^
PleI 163 GACTC GAGTC(4/5)
Sau3AI 100 GATC ^GATC
Sau96I 89 GGACC G^G[ACGT]CC
SmaI 103 CCCGGG CCC^GGG
SpeI 354 ACTAGT A^CTAGT
TspRI 177 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 99 GGATCC [GA]^GATC[CT]
XmaCI 103 CCCGGG C^CCGGG
XmaI 103 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 100 GATCC GGATC(4/5)
99 GGATC
BbvI 18 GCTGC GCAGC(8/12)
21 GCTGC
BsmFI 59 GGGAC GGGAC(10/14)
88 GGGAC
BsrI 178 ACTGG ACTGG(1/-1)
295 CCAGT
BstF5I 153 GGATG GGATG(2/0)
266 GGATG
CfoI 64 GCGC GCG^C
123 GCGC
EcoT14I 333 CCTTGG C^C[AT][AT]GG
348 CCTTGG
FokI 153 GGATG GGATG(9/13)
266 GGATG
HhaI 64 GCGC GCG^C
123 GCGC
HinP1I 64 GCGC G^CGC
123 GCGC
HphI 181 GGTGA GGTGA(8/7)
226 GGTGA
MnlI 52 GAGG CCTC(7/6)
79 GAGG
MslI 175 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
261 CACAAGGATG
MspA1I 115 CAGCGG C[AC]G^C[GT]G
130 CAGCGG
StyI 333 CCTTGG C^C[AT][AT]GG
348 CCTTGG
TseI 18 GCTGC G^C[AT]GC
21 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AccII AciI BcnI BsaJI BssKI
BstUI Cac8I FauI Fnu4HI HapII
HpaII ItaI MspI MvnI MwoI
NciI NlaIV ScrFI