Restriction map for
pFN21AE3149
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstYI
BstZ17I Bsu36I CfoI Cfr10I CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FokI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 869 GGTACC G^GTACC
AcsI 211 AAATTT [GA]^AATT[CT]
ApoI 211 AAATTT [GA]^AATT[CT]
Asp718I 869 GGTACC G^GTACC
AspHI 563 GTGCTC G[AT]GC[AT]^C
AvaI 929 CTCGAG C^[CT]CG[GA]G
AvaII 309 GGTCC G^G[AT]CC
BalI 881 TGGCCA TGG^CCA
BbsI 1075 GAAGAC GAAGAC(2/6)
BfaI 220 CTAG C^TAG
BlpI 324 GCTTAGC GC^T[ACGT]AGC
BmyI 563 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 324 GCTTAGC GC^T[ACGT]AGC
BpuAI 1075 GAAGAC GAAGAC(2/6)
BsaI 903 GGTCTC GGTCTC(1/5)
BsaJI 317 CCAAGG C^C[ACGT][ACGT]GG
BsiHKAI 563 GTGCTC G[AT]GC[AT]^C
BsoBI 929 CTCGAG C^[CT]CG[GA]G
Bsp1286I 563 GTGCTC G[AGT]GC[ACT]^C
BspDI 823 ATCGAT AT^CGAT
BspHI 284 TCATGA T^CATGA
BstXI 217 CCACTAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 764 GCTGGC GC[ACGT]^[ACGT]GC
CelII 324 GCTTAGC GC^T[ACGT]AGC
Cfr13I 309 GGTCC G^G[ACGT]CC
ClaI 823 ATCGAT AT^CGAT
DraII 308 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 429 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 308 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 4 GATATC GAT^ATC
EcoT14I 317 CCAAGG C^C[AT][AT]GG
EcoT22I 550 ATGCAT ATGCA^T
FauI 79 CCCGC CCCGC(4/6)
HgaI 467 GACGC GACGC(5/10)
KpnI 869 GGTACC GGTAC^C
MaeI 220 CTAG C^TAG
MluNI 881 TGGCCA TGG^CCA
MscI 881 TGGCCA TGG^CCA
MseI 757 TTAA T^TAA
NsiI 550 ATGCAT ATGCA^T
PaeR7I 929 CTCGAG C^TCGAG
PflMI 873 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 583 GACTC GAGTC(4/5)
PpuMI 308 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 276 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 284 TCATGA T^CATGA
Sau96I 309 GGTCC G^G[ACGT]CC
SmlI 929 CTCGAG C^T[CT][GA]AG
SspI 725 AATATT AAT^ATT
StyI 317 CCAAGG C^C[AT][AT]GG
Tru9I 757 TTAA T^TAA
Van91I 873 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 217 CCACTAGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 929 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 433 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
471 CAGATTCTG
BanI 154 GGCACC G^G[CT][GA]CC
869 GGTACC
BclI 287 TGATCA T^GATCA
830 TGATCA
BcnI 168 CCCGG CC^[GC]GG
782 CCGGG
BsmFI 465 GGGAC GGGAC(10/14)
310 GTCCC
BsmI 548 GAATGC GAATGC(1/-1)
794 GCATTC
Bsp1407I 297 TGTACA T^GTACA
972 TGTACA
BsrGI 297 TGTACA T^GTACA
972 TGTACA
EaeI 881 TGGCCA [CT]^GGCC[GA]
1003 TGGCCG
EarI 673 CTCTTC CTCTTC(1/4)
906 CTCTTC
FbaI 287 TGATCA T^GATCA
830 TGATCA
HapII 169 CCGG C^CGG
782 CCGG
HpaII 169 CCGG C^CGG
782 CCGG
Ksp632I 673 CTCTTC CTCTTC(1/4)
906 CTCTTC
MslI 218 CACTAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
513 CATTACAATG
MspI 169 CCGG C^CGG
782 CCGG
NciI 168 CCCGG CC^[GC]GG
782 CCGGG
SspBI 297 TGTACA T^GTACA
972 TGTACA
Tsp45I 683 GTCAC ^GT[GC]AC
737 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsiYI BslI BsmAI BsrDI BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI TaqI TfiI
TseI Tsp509I TspRI TthHB8I