Restriction map for
pFN21AE3132
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 394 GGTACC G^GTACC
AccI 366 GTCTAC GT^[AC][GT]AC
AciI 39 CCGC CCGC(-3/-1)
AcsI 281 AAATTC [GA]^AATT[CT]
ApoI 281 AAATTC [GA]^AATT[CT]
Asp718I 394 GGTACC G^GTACC
AvaII 33 GGTCC G^G[AT]CC
BanI 394 GGTACC G^G[CT][GA]CC
BanII 977 GGGCTC G[GA]GC[CT]^C
BbvI 150 GCAGC GCAGC(8/12)
BcgI 544 CGAATTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 977 GGGCTC G[AGT]GC[ACT]^C
BseRI 791 CTCCTC GAGGAG(10/8)
Bsp1286I 977 GGGCTC G[AGT]GC[ACT]^C
BspHI 44 TCATGA T^CATGA
BspMI 714 ACCTGC ACCTGC(4/8)
BsrDI 745 GCAATG GCAATG(2/0)
BsrI 102 ACTGG ACTGG(1/-1)
BstBI 542 TTCGAA TT^CGAA
BstF5I 686 GGATG GGATG(2/0)
Cfr13I 33 GGTCC G^G[ACGT]CC
DsaI 29 CCGTGG C^C[GA][CT]GG
EcoT14I 628 CCAAGG C^C[AT][AT]GG
Fnu4HI 150 GCAGC GC^[ACGT]GC
FokI 686 GGATG GGATG(9/13)
HaeIII 646 GGCC GG^CC
ItaI 150 GCAGC GC^[ACGT]GC
KpnI 394 GGTACC GGTAC^C
NspV 542 TTCGAA TT^CGAA
PleI 508 GACTC GAGTC(4/5)
PshAI 633 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 44 TCATGA T^CATGA
Sau96I 33 GGTCC G^G[ACGT]CC
SfcI 368 CTACAG C^T[GA][CT]AG
SfuI 542 TTCGAA TT^CGAA
StyI 628 CCAAGG C^C[AT][AT]GG
TseI 150 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 320 GTAC GT^AC
395 GTAC
AflII 145 CTTAAG C^TTAAG
166 CTTAAG
AlwI 891 GATCC GGATC(4/5)
537 GGATC
BfaI 566 CTAG C^TAG
761 CTAG
BfrI 145 CTTAAG C^TTAAG
166 CTTAAG
BpmI 103 CTGGAG CTGGAG(16/14)
812 CTGGAG
BsgI 328 CTGCAC GTGCAG(16/14)
116 GTGCAG
BsiYI 57 CCGTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
793 CCTCCAAATGG
BslI 57 CCGTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
793 CCTCCAAATGG
BsmBI 58 CGTCTC CGTCTC(1/5)
425 GAGACG
BstYI 537 GGATCT [GA]^GATC[CT]
890 AGATCC
Cac8I 186 GCAGGC GC[ACGT]^[ACGT]GC
330 GCACGC
DdeI 512 CTAAG C^T[ACGT]AG
869 CTAAG
EarI 96 GAAGAG CTCTTC(1/4)
202 GAAGAG
Ksp632I 96 GAAGAG CTCTTC(1/4)
202 GAAGAG
MaeI 566 CTAG C^TAG
761 CTAG
MaeIII 376 GTAAC ^GT[ACGT]AC
704 GTAAC
MflI 537 GGATCT [GA]^GATC[CT]
890 AGATCC
MseI 146 TTAA T^TAA
167 TTAA
MslI 574 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
907 CACACTAATG
RsaI 320 GTAC GT^AC
395 GTAC
TaqI 216 TCGA T^CGA
543 TCGA
Tru9I 146 TTAA T^TAA
167 TTAA
TthHB8I 216 TCGA T^CGA
543 TCGA
XhoII 537 GGATCT [GA]^GATC[CT]
890 AGATCC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BsaJI BsmAI BssKI BstNI
DpnII EcoRII HinfI HphI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
SmlI TfiI Tsp509I TspRI