################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 394 GGTACC G^GTACC AccI 366 GTCTAC GT^[AC][GT]AC AciI 39 CCGC CCGC(-3/-1) AcsI 281 AAATTC [GA]^AATT[CT] ApoI 281 AAATTC [GA]^AATT[CT] Asp718I 394 GGTACC G^GTACC AvaII 33 GGTCC G^G[AT]CC BanI 394 GGTACC G^G[CT][GA]CC BanII 977 GGGCTC G[GA]GC[CT]^C BbvI 150 GCAGC GCAGC(8/12) BcgI 544 CGAATTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 977 GGGCTC G[AGT]GC[ACT]^C BseRI 791 CTCCTC GAGGAG(10/8) Bsp1286I 977 GGGCTC G[AGT]GC[ACT]^C BspHI 44 TCATGA T^CATGA BspMI 714 ACCTGC ACCTGC(4/8) BsrDI 745 GCAATG GCAATG(2/0) BsrI 102 ACTGG ACTGG(1/-1) BstBI 542 TTCGAA TT^CGAA BstF5I 686 GGATG GGATG(2/0) Cfr13I 33 GGTCC G^G[ACGT]CC DsaI 29 CCGTGG C^C[GA][CT]GG EcoT14I 628 CCAAGG C^C[AT][AT]GG Fnu4HI 150 GCAGC GC^[ACGT]GC FokI 686 GGATG GGATG(9/13) HaeIII 646 GGCC GG^CC ItaI 150 GCAGC GC^[ACGT]GC KpnI 394 GGTACC GGTAC^C NspV 542 TTCGAA TT^CGAA PleI 508 GACTC GAGTC(4/5) PshAI 633 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 44 TCATGA T^CATGA Sau96I 33 GGTCC G^G[ACGT]CC SfcI 368 CTACAG C^T[GA][CT]AG SfuI 542 TTCGAA TT^CGAA StyI 628 CCAAGG C^C[AT][AT]GG TseI 150 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 320 GTAC GT^AC 395 GTAC AflII 145 CTTAAG C^TTAAG 166 CTTAAG AlwI 891 GATCC GGATC(4/5) 537 GGATC BfaI 566 CTAG C^TAG 761 CTAG BfrI 145 CTTAAG C^TTAAG 166 CTTAAG BpmI 103 CTGGAG CTGGAG(16/14) 812 CTGGAG BsgI 328 CTGCAC GTGCAG(16/14) 116 GTGCAG BsiYI 57 CCGTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 793 CCTCCAAATGG BslI 57 CCGTCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 793 CCTCCAAATGG BsmBI 58 CGTCTC CGTCTC(1/5) 425 GAGACG BstYI 537 GGATCT [GA]^GATC[CT] 890 AGATCC Cac8I 186 GCAGGC GC[ACGT]^[ACGT]GC 330 GCACGC DdeI 512 CTAAG C^T[ACGT]AG 869 CTAAG EarI 96 GAAGAG CTCTTC(1/4) 202 GAAGAG Ksp632I 96 GAAGAG CTCTTC(1/4) 202 GAAGAG MaeI 566 CTAG C^TAG 761 CTAG MaeIII 376 GTAAC ^GT[ACGT]AC 704 GTAAC MflI 537 GGATCT [GA]^GATC[CT] 890 AGATCC MseI 146 TTAA T^TAA 167 TTAA MslI 574 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 907 CACACTAATG RsaI 320 GTAC GT^AC 395 GTAC TaqI 216 TCGA T^CGA 543 TCGA Tru9I 146 TTAA T^TAA 167 TTAA TthHB8I 216 TCGA T^CGA 543 TCGA XhoII 537 GGATCT [GA]^GATC[CT] 890 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI BsaJI BsmAI BssKI BstNI DpnII EcoRII HinfI HphI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI SmlI TfiI Tsp509I TspRI