Restriction map for
pFN21AE1147
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AciI 679 CCGC CCGC(-3/-1)
AlwNI 689 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1138 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 119 CTCGGG C^[CT]CG[GA]G
BanII 208 GAGCCC G[GA]GC[CT]^C
BbvCI 1283 CCTCAGC CCTCAGC(-5/-2)
BpmI 713 CTCCAG CTGGAG(16/14)
BseRI 439 GAGGAG GAGGAG(10/8)
BsiYI 606 CCCTCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 606 CCCTCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 60 GCATTC GAATGC(1/-1)
BsoBI 119 CTCGGG C^[CT]CG[GA]G
BstAPI 880 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 726 AGATCC [GA]^GATC[CT]
DraI 1077 TTTAAA TTT^AAA
DsaI 464 CCATGG C^C[GA][CT]GG
HphI 582 GGTGA GGTGA(8/7)
MflI 726 AGATCC [GA]^GATC[CT]
MslI 465 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 716 CAGCTG C[AC]G^C[GT]G
NcoI 464 CCATGG C^CATGG
NlaIII 465 CATG CATG^
PflFI 1138 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 695 CTGCAG CTGCA^G
PvuII 716 CAGCTG CAG^CTG
ScaI 1243 AGTACT AGT^ACT
SfcI 695 CTGCAG C^T[GA][CT]AG
SmlI 1378 CTCAAG C^T[CT][GA]AG
SpeI 859 ACTAGT A^CTAGT
Tth111I 1138 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 726 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 173 AAATTT [GA]^AATT[CT]
825 AAATTC
AlwI 28 GATCC GGATC(4/5)
727 GATCC
ApoI 173 AAATTT [GA]^AATT[CT]
825 AAATTC
Asp700I 824 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1028 GAATAGGTTC
BbsI 227 GTCTTC GAAGAC(2/6)
1157 GTCTTC
BmyI 208 GAGCCC G[AGT]GC[ACT]^C
626 GTGCCC
BpuAI 227 GTCTTC GAAGAC(2/6)
1157 GTCTTC
BsaI 294 GAGACC GGTCTC(1/5)
442 GAGACC
Bsp1286I 208 GAGCCC G[AGT]GC[ACT]^C
626 GTGCCC
BspMI 297 ACCTGC ACCTGC(4/8)
960 ACCTGC
BstXI 464 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1197 CCAACTAAGTGG
Cac8I 481 GCCTGC GC[ACGT]^[ACGT]GC
838 GCCAGC
DpnII 28 GATC ^GATC
727 GATC
DraII 254 AGGTCCT [GA]G^G[ACGT]CC[CT]
1230 AGGACCT
EcoO109I 254 AGGTCCT [GA]G^G[ACGT]CC[CT]
1230 AGGACCT
EcoT14I 464 CCATGG C^C[AT][AT]GG
1100 CCTTGG
MboI 28 GATC ^GATC
727 GATC
NdeII 28 GATC ^GATC
727 GATC
PpuMI 254 AGGTCCT [GA]G^G[AT]CC[CT]
1230 AGGACCT
Sau3AI 28 GATC ^GATC
727 GATC
SfaNI 511 GCATC GCATC(5/9)
1288 GCATC
StyI 464 CCATGG C^C[AT][AT]GG
1100 CCTTGG
Tsp45I 583 GTGAC ^GT[GC]AC
1136 GTGAC
XmnI 824 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1028 GAATAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BsaJI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIV RsaI Sau96I ScrFI
TfiI Tru9I TseI Tsp509I TspRI