Restriction map for
pFN21AE2296
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaI AvrII BamHI
BbeI BbrPI BbsI BcgI BcnI
BfrI BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FauI FseI HaeII HapII Hin1I
HpaI HpaII KasI KpnI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 1401 ACATGT A^C[GA][CT]GT
Alw44I 1978 GTGCAC G^TGCAC
ApaLI 1978 GTGCAC G^TGCAC
Asp700I 2347 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 38 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1941 GGACC G^G[AT]CC
AviII 2733 TGCGCA TGC^GCA
BalI 2328 TGGCCA TGG^CCA
BanI 1368 GGCACC G^G[CT][GA]CC
BbvCI 1246 CCTCAGC CCTCAGC(-5/-2)
BciVI 3360 GGATAC GTATCC(6/5)
BclI 2301 TGATCA T^GATCA
BglI 1833 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2743 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2743 GCTCAGC GC^T[ACGT]AGC
BsaI 3076 GAGACC GGTCTC(1/5)
BspLU11I 1401 ACATGT A^CATGT
BspMI 3036 ACCTGC ACCTGC(4/8)
BssSI 1646 CACGAG CACGAG(-5/-1)
BstAPI 2586 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2743 GCTCAGC GC^T[ACGT]AGC
CfoI 2734 GCGC GCG^C
DraI 1110 TTTAAA TTT^AAA
DraII 1703 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 2328 TGGCCA [CT]^GGCC[GA]
EcoNI 1255 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1703 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1051 GAATTC G^AATTC
FbaI 2301 TGATCA T^GATCA
FspI 2733 TGCGCA TGC^GCA
HgaI 1039 GCGTC GACGC(5/10)
HhaI 2734 GCGC GCG^C
HinP1I 2734 GCGC G^CGC
MfeI 1207 CAATTG C^AATTG
MluNI 2328 TGGCCA TGG^CCA
MscI 2328 TGGCCA TGG^CCA
MunI 1207 CAATTG C^AATTG
NcoI 3080 CCATGG C^CATGG
NheI 1035 GCTAGC G^CTAGC
PflFI 38 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1549 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1517 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 128 GAGCTC GAGCT^C
SexAI 1973 ACCAGGT A^CC[AT]GGT
SmlI 1534 CTCAAG C^T[CT][GA]AG
Tth111I 38 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1549 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2347 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 2616 GATCC GGATC(4/5)
2317 GGATC
AocI 809 CCTCAGG CC^T[ACGT]AGG
1363 CCTGAGG
BglII 1650 AGATCT A^GATCT
2628 AGATCT
BseRI 1226 CTCCTC GAGGAG(10/8)
1229 CTCCTC
BsrDI 717 CATTGC GCAATG(2/0)
925 GCAATG
BstYI 1650 AGATCT [GA]^GATC[CT]
2628 AGATCT
Bsu36I 809 CCTCAGG CC^T[ACGT]AGG
1363 CCTGAGG
DsaI 650 CCGTGG C^C[GA][CT]GG
3080 CCATGG
EarI 645 CTCTTC CTCTTC(1/4)
1232 CTCTTC
Eco81I 809 CCTCAGG CC^T[ACGT]AGG
1363 CCTGAGG
HincII 410 GTCAAC GT[CT]^[GA]AC
2723 GTCAAC
HindII 410 GTCAAC GT[CT]^[GA]AC
2723 GTCAAC
HindIII 1853 AAGCTT A^AGCTT
2675 AAGCTT
Ksp632I 645 CTCTTC CTCTTC(1/4)
1232 CTCTTC
MaeII 366 ACGT A^CGT
1740 ACGT
MflI 1650 AGATCT [GA]^GATC[CT]
2628 AGATCT
SphI 2479 GCATGC GCATG^C
2844 GCATGC
SspI 695 AATATT AAT^ATT
2597 AATATT
StuI 881 AGGCCT AGG^CCT
1435 AGGCCT
TaqI 1629 TCGA T^CGA
2390 TCGA
TthHB8I 1629 TCGA T^CGA
2390 TCGA
XcmI 345 CCAAAAGGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2709 CCAAGATATGTCTGG
XhoII 1650 AGATCT [GA]^GATC[CT]
2628 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BanII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraIII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI