Restriction map for
pFN21AB5877
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1009 GTCTAC GT^[AC][GT]AC
AcsI 1326 GAATTC [GA]^AATT[CT]
AflIII 413 ACACGT A^C[GA][CT]GT
ApoI 1326 GAATTC [GA]^AATT[CT]
BanI 460 GGCGCC G^G[CT][GA]CC
BbeI 460 GGCGCC GGCGC^C
BcnI 89 CCGGG CC^[GC]GG
BsaI 1295 GAGACC GGTCTC(1/5)
BspMI 1005 GCAGGT ACCTGC(4/8)
BsrFI 1184 ACCGGC [GA]^CCGG[CT]
BstXI 533 CCATTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1184 ACCGGC [GA]^CCGG[CT]
DdeI 650 CTCAG C^T[ACGT]AG
DraII 717 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 717 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1326 GAATTC G^AATTC
EcoT14I 398 CCATGG C^C[AT][AT]GG
FauI 193 CCCGC CCCGC(4/6)
HaeII 460 GGCGCC [GA]GCGC^[CT]
KasI 460 GGCGCC G^GCGCC
MaeII 415 ACGT A^CGT
MboII 1275 GAAGA GAAGA(8/7)
MspA1I 500 CAGCTG C[AC]G^C[GT]G
NarI 460 GGCGCC GG^CGCC
NciI 89 CCGGG CC^[GC]GG
NcoI 398 CCATGG C^CATGG
PleI 1219 GAGTC GAGTC(4/5)
PpuMI 717 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 500 CAGCTG CAG^CTG
SfcI 590 CTACAG C^T[GA][CT]AG
SfoI 460 GGCGCC GGC^GCC
SmlI 1159 CTCAAG C^T[CT][GA]AG
SspI 1388 AATATT AAT^ATT
StyI 398 CCATGG C^C[AT][AT]GG
TaqI 1089 TCGA T^CGA
TfiI 624 GATTC G^A[AT]TC
TthHB8I 1089 TCGA T^CGA
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 565 GATCC GGATC(4/5)
323 GGATC
AspHI 1038 GAGCTC G[AT]GC[AT]^C
1156 GAGCTC
BalI 2 TGGCCA TGG^CCA
709 TGGCCA
BanII 1038 GAGCTC G[GA]GC[CT]^C
1156 GAGCTC
BmyI 1038 GAGCTC G[AGT]GC[ACT]^C
1156 GAGCTC
BsgI 53 GTGCAG GTGCAG(16/14)
235 GTGCAG
BsiHKAI 1038 GAGCTC G[AT]GC[AT]^C
1156 GAGCTC
BsiYI 203 CCCAGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCAGCCCTGGG
BslI 203 CCCAGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCAGCCCTGGG
BsmAI 112 GAGAC GTCTC(1/5)
1295 GAGAC
Bsp1286I 1038 GAGCTC G[AGT]GC[ACT]^C
1156 GAGCTC
Bsp1407I 1049 TGTACA T^GTACA
1199 TGTACA
BspHI 861 TCATGA T^CATGA
914 TCATGA
BsrGI 1049 TGTACA T^GTACA
1199 TGTACA
DpnII 324 GATC ^GATC
565 GATC
DsaI 98 CCACGG C^C[GA][CT]GG
398 CCATGG
HapII 89 CCGG C^CGG
1185 CCGG
HgaI 58 GACGC GACGC(5/10)
391 GACGC
HinfI 624 GATTC G^A[ACGT]TC
1219 GAGTC
HpaII 89 CCGG C^CGG
1185 CCGG
MboI 324 GATC ^GATC
565 GATC
MluNI 2 TGGCCA TGG^CCA
709 TGGCCA
MscI 2 TGGCCA TGG^CCA
709 TGGCCA
MslI 498 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
534 CATTGCGGTG
MspI 89 CCGG C^CGG
1185 CCGG
NdeII 324 GATC ^GATC
565 GATC
NheI 81 GCTAGC G^CTAGC
1018 GCTAGC
RcaI 861 TCATGA T^CATGA
914 TCATGA
SacI 1038 GAGCTC GAGCT^C
1156 GAGCTC
Sau3AI 324 GATC ^GATC
565 GATC
SexAI 1168 ACCAGGT A^CC[AT]GGT
1261 ACCAGGT
SspBI 1049 TGTACA T^GTACA
1199 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BfaI BglI BsaHI
BsaJI BseRI BsmFI BsrDI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I EaeI EcoRII Fnu4HI
FokI HaeIII HhaI Hin1I HinP1I
HphI ItaI MaeI MaeIII MnlI
MvaI MvnI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI