Restriction map for
pFN21AE5654
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvrII BbrPI BbsI BbvCI BciVI
BcnI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI MamI MluI MroI NaeI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 109 GACGTC GACGT^C
AccI 1297 GTATAC GT^[AC][GT]AC
AccII 206 CGCG CG^CG
AflII 688 CTTAAG C^TTAAG
AocI 273 CCTGAGG CC^T[ACGT]AGG
AspHI 993 GAGCAC G[AT]GC[AT]^C
AviII 96 TGCGCA TGC^GCA
BamHI 388 GGATCC G^GATCC
BanII 156 GAGCCC G[GA]GC[CT]^C
BbeI 79 GGCGCC GGCGC^C
BcgI 1068 CGACACCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1512 TGATCA T^GATCA
BfaI 664 CTAG C^TAG
BfrI 688 CTTAAG C^TTAAG
BglI 187 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 409 GAGGAG GAGGAG(10/8)
BsgI 145 CTGCAC GTGCAG(16/14)
BsiHKAI 993 GAGCAC G[AT]GC[AT]^C
BsmAI 261 GTCTC GTCTC(1/5)
BspMI 93 ACCTGC ACCTGC(4/8)
Bst1107I 1297 GTATAC GTA^TAC
BstEII 1394 GGTTACC G^GT[ACGT]ACC
BstPI 1394 GGTTACC G^GT[ACGT]ACC
BstUI 206 CGCG CG^CG
BstXI 1479 CCAACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 388 GGATCC [GA]^GATC[CT]
BstZ17I 1297 GTATAC GTA^TAC
Bsu36I 273 CCTGAGG CC^T[ACGT]AGG
DraIII 148 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 273 CCTGAGG CC^T[ACGT]AGG
EcoNI 687 CCTTAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1394 GGTTACC G^GT[ACGT]ACC
FauI 204 CCCGC CCCGC(4/6)
FbaI 1512 TGATCA T^GATCA
FspI 96 TGCGCA TGC^GCA
HapII 1548 CCGG C^CGG
HgaI 184 GACGC GACGC(5/10)
HincII 364 GTCAAC GT[CT]^[GA]AC
HindII 364 GTCAAC GT[CT]^[GA]AC
HpaII 1548 CCGG C^CGG
KasI 79 GGCGCC G^GCGCC
KspI 205 CCGCGG CCGC^GG
MaeI 664 CTAG C^TAG
MaeII 110 ACGT A^CGT
MflI 388 GGATCC [GA]^GATC[CT]
MslI 1245 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1548 CCGG C^CGG
MvnI 206 CGCG CG^CG
NarI 79 GGCGCC GG^CGCC
NdeI 1040 CATATG CA^TATG
PflMI 1173 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1079 CAGCTG CAG^CTG
SacII 205 CCGCGG CCGC^GG
ScaI 1385 AGTACT AGT^ACT
SexAI 149 ACCTGGT A^CC[AT]GGT
SfcI 506 CTGTAG C^T[GA][CT]AG
SfoI 79 GGCGCC GGC^GCC
StuI 1000 AGGCCT AGG^CCT
Van91I 1173 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1247 CCAAGGTGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 388 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 194 TGGCCA TGG^CCA
248 TGGCCA
BsmFI 1449 GGGAC GGGAC(10/14)
65 GTCCC
BsrDI 1306 CATTGC GCAATG(2/0)
668 GCAATG
DraII 63 GGGTCCC [GA]G^G[ACGT]CC[CT]
1449 GGGACCT
DsaI 197 CCACGG C^C[GA][CT]GG
205 CCGCGG
EcoO109I 63 GGGTCCC [GA]G^G[ACGT]CC[CT]
1449 GGGACCT
HaeII 79 GGCGCC [GA]GCGC^[CT]
1053 AGCGCC
HindIII 235 AAGCTT A^AGCTT
1288 AAGCTT
HinfI 325 GAATC G^A[ACGT]TC
871 GATTC
MfeI 445 CAATTG C^AATTG
742 CAATTG
MluNI 194 TGGCCA TGG^CCA
248 TGGCCA
MscI 194 TGGCCA TGG^CCA
248 TGGCCA
MunI 445 CAATTG C^AATTG
742 CAATTG
NlaIII 881 CATG CATG^
1456 CATG
PpuMI 63 GGGTCCC [GA]G^G[AT]CC[CT]
1449 GGGACCT
SapI 837 GAAGAGC GCTCTTC(1/4)
632 GCTCTTC
TfiI 325 GAATC G^A[AT]TC
871 GATTC
Tsp45I 175 GTCAC ^GT[GC]AC
1378 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
Asp700I AvaII BanI BbvI BmyI
BsaHI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI Hin1I HinP1I HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI