Restriction map for
pFN21AE3422
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccIII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII Hin1I HindIII
HpaI HpaII KasI Ksp632I KspI
MaeII MfeI MflI MluI MroI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TspRI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 227 GGTACC G^GTACC
AccII 443 CGCG CG^CG
AcsI 241 GAATTT [GA]^AATT[CT]
AlwI 1033 GATCC GGATC(4/5)
ApoI 241 GAATTT [GA]^AATT[CT]
Asp700I 926 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 227 GGTACC G^GTACC
BalI 89 TGGCCA TGG^CCA
BanI 227 GGTACC G^G[CT][GA]CC
BciVI 1020 GGATAC GTATCC(6/5)
BclI 624 TGATCA T^GATCA
BlpI 835 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 835 GCTGAGC GC^T[ACGT]AGC
BsaBI 619 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 1291 GGGAC GGGAC(10/14)
BstUI 443 CGCG CG^CG
Cac8I 1123 GCCAGC GC[ACGT]^[ACGT]GC
CelII 835 GCTGAGC GC^T[ACGT]AGC
CfoI 442 GCGC GCG^C
DraI 1060 TTTAAA TTT^AAA
DraII 1422 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 89 TGGCCA [CT]^GGCC[GA]
EcoO109I 1422 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 667 CCAAGG C^C[AT][AT]GG
FbaI 624 TGATCA T^GATCA
HgaI 752 GACGC GACGC(5/10)
HhaI 442 GCGC GCG^C
HinP1I 442 GCGC G^CGC
HincII 247 GTTGAC GT[CT]^[GA]AC
HindII 247 GTTGAC GT[CT]^[GA]AC
KpnI 227 GGTACC GGTAC^C
MamI 619 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 89 TGGCCA TGG^CCA
MscI 89 TGGCCA TGG^CCA
MslI 1398 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1125 CAGCTG C[AC]G^C[GT]G
MvnI 443 CGCG CG^CG
PpuMI 1422 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1125 CAGCTG CAG^CTG
StyI 667 CCAAGG C^C[AT][AT]GG
XcmI 560 CCATTGGTTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 926 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 455 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1445 CAGGGGCTG
AvaII 1206 GGACC G^G[AT]CC
1423 GGACC
BbvI 454 GCAGC GCAGC(8/12)
1190 GCAGC
BfaI 232 CTAG C^TAG
292 CTAG
BsiYI 18 CCTGGGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1306 CCAATTCAGGG
BslI 18 CCTGGGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1306 CCAATTCAGGG
BsrDI 142 GCAATG GCAATG(2/0)
1193 GCAATG
BsrI 76 ACTGG ACTGG(1/-1)
405 CCAGT
BstF5I 867 GGATG GGATG(2/0)
1326 GGATG
DpnII 625 GATC ^GATC
1033 GATC
Fnu4HI 454 GCAGC GC^[ACGT]GC
1190 GCAGC
FokI 867 GGATG GGATG(9/13)
1326 GGATG
HaeIII 90 GGCC GG^CC
558 GGCC
ItaI 454 GCAGC GC^[ACGT]GC
1190 GCAGC
MaeI 232 CTAG C^TAG
292 CTAG
MboI 625 GATC ^GATC
1033 GATC
MboII 80 GAAGA GAAGA(8/7)
994 GAAGA
NdeII 625 GATC ^GATC
1033 GATC
NlaIV 227 GGTACC GG[ACGT]^[ACGT]CC
594 GGAACC
PstI 1188 CTGCAG CTGCA^G
1259 CTGCAG
Sau3AI 625 GATC ^GATC
1033 GATC
SfaNI 919 GATGC GCATC(5/9)
672 GCATC
TseI 454 GCAGC G^C[AT]GC
1190 GCAGC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AseI AsnI AspHI
BanII BmyI BpmI BsaJI BsiHKAI
BsmAI Bsp1286I BssKI BstNI Cfr13I
DdeI EcoRII HinfI HphI MaeIII
MnlI MseI MvaI MwoI NlaIII
PshBI RsaI SacI Sau96I ScrFI
SfcI TfiI Tru9I Tsp45I Tsp509I