Restriction map for
pFN21AE6524
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI ItaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I TseI Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 112 GCGG CCGC(-3/-1)
AhdI 263 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 373 AGCT AG^CT
AlwNI 9 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 179 GGGCCC GGGCC^C
AspEI 263 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 159 CCCGAG C^[CT]CG[GA]G
BalI 316 TGGCCA TGG^CCA
BanII 179 GGGCCC G[GA]GC[CT]^C
BbsI 356 GAAGAC GAAGAC(2/6)
BglII 131 AGATCT A^GATCT
BmyI 179 GGGCCC G[AGT]GC[ACT]^C
BpuAI 356 GAAGAC GAAGAC(2/6)
BsaWI 168 TCCGGT [AT]^CCGG[AT]
BseRI 162 GAGGAG GAGGAG(10/8)
BsoBI 159 CCCGAG C^[CT]CG[GA]G
Bsp1286I 179 GGGCCC G[AGT]GC[ACT]^C
BspMI 153 ACCTGC ACCTGC(4/8)
BstAPI 8 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 307 CCATTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 131 AGATCT [GA]^GATC[CT]
Cac8I 4 GCTCGC GC[ACGT]^[ACGT]GC
DdeI 380 CTGAG C^T[ACGT]AG
DpnII 132 GATC ^GATC
DraII 178 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 316 TGGCCA [CT]^GGCC[GA]
Eam1105I 263 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 73 GAAGAG CTCTTC(1/4)
EcoO109I 178 AGGGCCC [GA]G^G[ACGT]CC[CT]
HapII 169 CCGG C^CGG
HgaI 115 GACGC GACGC(5/10)
HpaII 169 CCGG C^CGG
HphI 143 TCACC GGTGA(8/7)
Ksp632I 73 GAAGAG CTCTTC(1/4)
MboI 132 GATC ^GATC
MflI 131 AGATCT [GA]^GATC[CT]
MluNI 316 TGGCCA TGG^CCA
MscI 316 TGGCCA TGG^CCA
MslI 200 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 169 CCGG C^CGG
MwoI 8 GCAGACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 132 GATC ^GATC
NlaIV 179 GGGCCC GG[ACGT]^[ACGT]CC
Sau3AI 132 GATC ^GATC
Tsp45I 172 GTCAC ^GT[GC]AC
TspRI 13 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 131 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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BpmI 361 CTCCAG CTGGAG(16/14)
92 CTGGAG
BsaJI 160 CCGAGG C^C[ACGT][ACGT]GG
202 CCCAGG
BsmFI 209 GTCCC GGGAC(10/14)
271 GTCCC
BsrI 26 ACTGG ACTGG(1/-1)
33 ACTGG
BssKI 91 CCTGG ^CC[ACGT]GG
203 CCAGG
BstNI 91 CCTGG CC^[AT]GG
203 CCAGG
EcoRII 91 CCTGG ^CC[AT]GG
203 CCAGG
MvaI 91 CCTGG CC^[AT]GG
203 CCAGG
PleI 359 GACTC GAGTC(4/5)
165 GAGTC
ScrFI 91 CCTGG CC^[ACGT]GG
203 CCAGG
TfiI 97 GATTC G^A[AT]TC
239 GAATC
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
Cfr13I HaeIII HinfI MaeIII MboII
MnlI Sau96I