Restriction map for
pFN21AE9546
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BseRI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HincII HindII HpaI
KasI KpnI MamI MluI MroI
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AfaI 384 GTAC GT^AC
AflIII 852 ACATGT A^C[GA][CT]GT
AlwNI 231 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 394 ATTAAT AT^TAAT
AsnI 394 ATTAAT AT^TAAT
Asp700I 3 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 284 GGGCTC G[GA]GC[CT]^C
BbrPI 1093 CACGTG CAC^GTG
BbsI 928 GAAGAC GAAGAC(2/6)
BclI 800 TGATCA T^GATCA
BglI 277 GCCGACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 956 AGATCT A^GATCT
BpuAI 928 GAAGAC GAAGAC(2/6)
BsaAI 1093 CACGTG [CT]AC^GT[GA]
BsaWI 66 ACCGGA [AT]^CCGG[AT]
BsgI 62 CTGCAC GTGCAG(16/14)
BsmBI 54 CGTCTC CGTCTC(1/5)
BsmI 1270 GCATTC GAATGC(1/-1)
BspHI 305 TCATGA T^CATGA
BspLU11I 852 ACATGT A^CATGT
BsrBI 132 GAGCGG CCGCTC(-3/-3)
BsrFI 191 GCCGGC [GA]^CCGG[CT]
BstAPI 590 GCATATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 910 CCAGTCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 956 AGATCT [GA]^GATC[CT]
Cfr10I 191 GCCGGC [GA]^CCGG[CT]
DraI 757 TTTAAA TTT^AAA
DraII 809 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1060 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 809 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 800 TGATCA T^GATCA
HgaI 104 GCGTC GACGC(5/10)
KspI 161 CCGCGG CCGC^GG
MfeI 719 CAATTG C^AATTG
MflI 956 AGATCT [GA]^GATC[CT]
MunI 719 CAATTG C^AATTG
NaeI 191 GCCGGC GCC^GGC
NdeI 529 CATATG CA^TATG
NgoMIV 191 GCCGGC G^CCGGC
PflMI 738 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1093 CACGTG CAC^GTG
PmlI 1093 CACGTG CAC^GTG
PpuMI 809 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 169 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 394 ATTAAT AT^TAAT
PvuII 30 CAGCTG CAG^CTG
RcaI 305 TCATGA T^CATGA
RsaI 384 GTAC GT^AC
SacII 161 CCGCGG CCGC^GG
SmlI 431 CTCAAG C^T[CT][GA]AG
SpeI 582 ACTAGT A^CTAGT
SphI 599 GCATGC GCATG^C
Van91I 738 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 956 AGATCT [GA]^GATC[CT]
XmnI 3 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 52 GACGTC GACGT^C
173 GACGTC
AccI 748 GTCTAC GT^[AC][GT]AC
913 GTCTAC
AccII 115 CGCG CG^CG
162 CGCG
AcsI 1034 GAATTT [GA]^AATT[CT]
1320 GAATTT
AcyI 52 GACGTC G[GA]^CG[CT]C
173 GACGTC
ApoI 1034 GAATTT [GA]^AATT[CT]
1320 GAATTT
AspHI 95 GAGCAC G[AT]GC[AT]^C
1417 GTGCTC
AvaII 675 GGTCC G^G[AT]CC
810 GGTCC
BanI 35 GGTGCC G^G[CT][GA]CC
920 GGTGCC
BcnI 291 CCGGG CC^[GC]GG
924 CCGGG
BfaI 583 CTAG C^TAG
814 CTAG
BsaHI 52 GACGTC G[GA]^CG[CT]C
173 GACGTC
BsiHKAI 95 GAGCAC G[AT]GC[AT]^C
1417 GTGCTC
BstF5I 346 CATCC GGATG(2/0)
634 GGATG
BstUI 115 CGCG CG^CG
162 CGCG
CfoI 90 GCGC GCG^C
114 GCGC
DraIII 651 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
1093 CACGTGGTG
DsaI 161 CCGCGG C^C[GA][CT]GG
1329 CCGTGG
FokI 346 CATCC GGATG(9/13)
634 GGATG
HhaI 90 GCGC GCG^C
114 GCGC
Hin1I 52 GACGTC G[GA]^CG[CT]C
173 GACGTC
HinP1I 90 GCGC G^CGC
114 GCGC
HindIII 1343 AAGCTT A^AGCTT
1388 AAGCTT
HphI 423 TCACC GGTGA(8/7)
821 TCACC
MaeI 583 CTAG C^TAG
814 CTAG
MvnI 115 CGCG CG^CG
162 CGCG
NciI 291 CCGGG CC^[GC]GG
924 CCGGG
NspI 599 GCATGC [GA]CATG^[CT]
852 ACATGT
PleI 884 GACTC GAGTC(4/5)
1179 GACTC
TfiI 261 GATTC G^A[AT]TC
1450 GAATC
Tsp45I 50 GTGAC ^GT[GC]AC
541 GTCAC
XcmI 22 CCAGCGGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
980 CCAACATCTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI BalI BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI Tru9I TseI
Tsp509I TspRI