Restriction map for
pFN21AE2417
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MflI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 913 GAATTT [GA]^AATT[CT]
AfaI 726 GTAC GT^AC
Alw44I 965 GTGCAC G^TGCAC
AocI 1069 CCTAAGG CC^T[ACGT]AGG
ApaLI 965 GTGCAC G^TGCAC
ApoI 913 GAATTT [GA]^AATT[CT]
Asp700I 424 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1303 CCCGAG C^[CT]CG[GA]G
BanI 501 GGCACC G^G[CT][GA]CC
BbsI 808 GAAGAC GAAGAC(2/6)
BbvCI 91 CCTCAGC CCTCAGC(-5/-2)
BciVI 1242 GGATAC GTATCC(6/5)
BpmI 379 CTCCAG CTGGAG(16/14)
BpuAI 808 GAAGAC GAAGAC(2/6)
BsaAI 727 TACGTG [CT]AC^GT[GA]
BsaBI 42 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 232 GGTCTC GGTCTC(1/5)
BseRI 235 CTCCTC GAGGAG(10/8)
BsoBI 1303 CCCGAG C^[CT]CG[GA]G
BspHI 776 TCATGA T^CATGA
BstXI 1208 CCACATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1069 CCTAAGG CC^T[ACGT]AGG
EarI 254 CTCTTC CTCTTC(1/4)
Eco81I 1069 CCTAAGG CC^T[ACGT]AGG
EcoT22I 1312 ATGCAT ATGCA^T
FauI 800 GCGGG CCCGC(4/6)
Ksp632I 254 CTCTTC CTCTTC(1/4)
MaeII 728 ACGT A^CGT
MamI 42 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 748 CAATTG C^AATTG
MslI 1209 CACATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1142 CAGCTG C[AC]G^C[GT]G
MunI 748 CAATTG C^AATTG
NsiI 1312 ATGCAT ATGCA^T
PflMI 838 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 157 CTGCAG CTGCA^G
PvuII 1142 CAGCTG CAG^CTG
RcaI 776 TCATGA T^CATGA
RsaI 726 GTAC GT^AC
SacI 529 GAGCTC GAGCT^C
SfcI 157 CTGCAG C^T[GA][CT]AG
SmlI 130 CTTGAG C^T[CT][GA]AG
SspI 299 AATATT AAT^ATT
TfiI 15 GATTC G^A[AT]TC
Van91I 838 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 424 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 800 GCGG CCGC(-3/-1)
896 GCGG
AspHI 529 GAGCTC G[AT]GC[AT]^C
965 GTGCAC
BsiHKAI 529 GAGCTC G[AT]GC[AT]^C
965 GTGCAC
BsmFI 450 GGGAC GGGAC(10/14)
1119 GGGAC
BsmI 753 GAATGC GAATGC(1/-1)
872 GAATGC
BspMI 1038 ACCTGC ACCTGC(4/8)
495 GCAGGT
BsrI 381 CCAGT ACTGG(1/-1)
925 CCAGT
Cac8I 796 GCAGGC GC[ACGT]^[ACGT]GC
1088 GCCTGC
HgaI 1318 GACGC GACGC(5/10)
1130 GCGTC
MaeIII 899 GTGAC ^GT[ACGT]AC
1132 GTCAC
MseI 166 TTAA T^TAA
212 TTAA
NcoI 259 CCATGG C^CATGG
1238 CCATGG
PleI 919 GACTC GAGTC(4/5)
1121 GACTC
PpuMI 35 AGGACCT [GA]G^G[AT]CC[CT]
1186 AGGACCT
Tru9I 166 TTAA T^TAA
212 TTAA
Tsp45I 899 GTGAC ^GT[GC]AC
1132 GTCAC
XbaI 187 TCTAGA T^CTAGA
1005 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AluI AlwI AlwNI AvaII BanII
BbvI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp509I TspRI