Restriction map for
pFN21AE1787
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AluI 650 AGCT AG^CT
Asp700I 331 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 454 GGATCC G^GATCC
BbrPI 554 CACGTG CAC^GTG
BbvI 840 GCAGC GCAGC(8/12)
BfaI 302 CTAG C^TAG
BsaAI 554 CACGTG [CT]AC^GT[GA]
BseRI 646 GAGGAG GAGGAG(10/8)
BsmAI 782 GAGAC GTCTC(1/5)
BspLU11I 519 ACATGT A^CATGT
BsrDI 123 CATTGC GCAATG(2/0)
BsrI 785 ACTGG ACTGG(1/-1)
BstBI 869 TTCGAA TT^CGAA
BstYI 454 GGATCC [GA]^GATC[CT]
DdeI 875 CTGAG C^T[ACGT]AG
DsaI 42 CCATGG C^C[GA][CT]GG
EcoNI 582 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 592 GATATC GAT^ATC
EcoT14I 42 CCATGG C^C[AT][AT]GG
Fnu4HI 840 GCAGC GC^[ACGT]GC
HapII 384 CCGG C^CGG
HpaII 384 CCGG C^CGG
ItaI 840 GCAGC GC^[ACGT]GC
MaeI 302 CTAG C^TAG
MaeII 555 ACGT A^CGT
MflI 454 GGATCC [GA]^GATC[CT]
MseI 224 TTAA T^TAA
MspI 384 CCGG C^CGG
MwoI 94 GCGTTCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 42 CCATGG C^CATGG
NspI 519 ACATGT [GA]CATG^[CT]
NspV 869 TTCGAA TT^CGAA
PflMI 445 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 554 CACGTG CAC^GTG
PmlI 554 CACGTG CAC^GTG
SfuI 869 TTCGAA TT^CGAA
SspI 74 AATATT AAT^ATT
StyI 42 CCATGG C^C[AT][AT]GG
Tru9I 224 TTAA T^TAA
TseI 840 GCAGC G^C[AT]GC
Van91I 445 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 454 GGATCC [GA]^GATC[CT]
XmnI 331 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 146 GTAC GT^AC
559 GTAC
AflIII 519 ACATGT A^C[GA][CT]GT
555 ACGTGT
AlwI 455 GATCC GGATC(4/5)
454 GGATC
BbsI 312 GAAGAC GAAGAC(2/6)
577 GAAGAC
BpuAI 312 GAAGAC GAAGAC(2/6)
577 GAAGAC
BsaJI 42 CCATGG C^C[ACGT][ACGT]GG
152 CCTGGG
BsmI 244 GAATGC GAATGC(1/-1)
387 GAATGC
BspHI 263 TCATGA T^CATGA
761 TCATGA
EcoRI 3 GAATTC G^AATTC
294 GAATTC
HphI 190 GGTGA GGTGA(8/7)
606 GGTGA
PleI 315 GACTC GAGTC(4/5)
549 GAGTC
RcaI 263 TCATGA T^CATGA
761 TCATGA
RsaI 146 GTAC GT^AC
559 GTAC
TaqI 228 TCGA T^CGA
870 TCGA
TfiI 48 GATTC G^A[AT]TC
853 GAATC
TspRI 722 CACTG CA[GC]TG[ACGT][ACGT]^
860 CACTG
TthHB8I 228 TCGA T^CGA
870 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI ApoI BsiYI BslI BssKI
BstF5I BstNI DpnII EcoRII FokI
HinfI MboI MboII MnlI MvaI
NdeII NlaIII NlaIV Sau3AI ScrFI
Tsp509I