Restriction map for
pFN21AE6450
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AlwNI
ApaI AscI Asp700I AspI AvaI
AvaII AviII AvrII BamHI BbeI
BbrPI BcgI BcnI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I Cfr10I Cfr13I ClaI CpoI
DraII DrdI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FseI FspI
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI Ksp632I KspI
MamI MfeI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI Sau96I SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 555 GGTACC G^GTACC
AflIII 662 ACATGT A^C[GA][CT]GT
AhdI 604 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 170 GTGCAC G^TGCAC
AocI 859 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1273 AGCGCT AGC^GCT
ApaLI 170 GTGCAC G^TGCAC
AseI 1291 ATTAAT AT^TAAT
AsnI 1291 ATTAAT AT^TAAT
Asp718I 555 GGTACC G^GTACC
AspEI 604 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 968 CCTCAGC CCTCAGC(-5/-2)
BciVI 1061 GGATAC GTATCC(6/5)
BfaI 157 CTAG C^TAG
BglII 371 AGATCT A^GATCT
BlpI 1269 GCTTAGC GC^T[ACGT]AGC
BpmI 521 CTCCAG CTGGAG(16/14)
Bpu1102I 1269 GCTTAGC GC^T[ACGT]AGC
BsmFI 1156 GTCCC GGGAC(10/14)
BsmI 1314 GAATGC GAATGC(1/-1)
BspLU11I 662 ACATGT A^CATGT
BspMI 843 ACCTGC ACCTGC(4/8)
BsrDI 1200 CATTGC GCAATG(2/0)
Bsu36I 859 CCTAAGG CC^T[ACGT]AGG
CelII 1269 GCTTAGC GC^T[ACGT]AGC
DraI 480 TTTAAA TTT^AAA
DraIII 355 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1149 CCACGG C^C[GA][CT]GG
Eam1105I 604 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1273 AGCGCT AGC^GCT
Eco81I 859 CCTAAGG CC^T[ACGT]AGG
HaeII 1273 AGCGCT [GA]GCGC^[CT]
KpnI 555 GGTACC GGTAC^C
MaeI 157 CTAG C^TAG
MaeII 727 ACGT A^CGT
NspI 662 ACATGT [GA]CATG^[CT]
PflMI 1458 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1154 GAGTC GAGTC(4/5)
PshBI 1291 ATTAAT AT^TAAT
SexAI 1444 ACCTGGT A^CC[AT]GGT
SmlI 510 CTTGAG C^T[CT][GA]AG
Van91I 1458 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
BalI 76 TGGCCA TGG^CCA
332 TGGCCA
BanI 225 GGTGCC G^G[CT][GA]CC
555 GGTACC
BanII 124 GAGCTC G[GA]GC[CT]^C
885 GAGCTC
BbsI 716 GAAGAC GAAGAC(2/6)
1375 GTCTTC
BbvI 1225 GCAGC GCAGC(8/12)
1429 GCAGC
BclI 1185 TGATCA T^GATCA
1344 TGATCA
BpuAI 716 GAAGAC GAAGAC(2/6)
1375 GTCTTC
BsaJI 1149 CCACGG C^C[ACGT][ACGT]GG
1361 CCCTGG
BsrI 451 CCAGT ACTGG(1/-1)
1452 CCAGT
BstF5I 1087 CATCC GGATG(2/0)
1467 GGATG
BstYI 371 AGATCT [GA]^GATC[CT]
1130 GGATCT
CfoI 1274 GCGC GCG^C
1398 GCGC
EaeI 76 TGGCCA [CT]^GGCC[GA]
332 TGGCCA
EcoRI 491 GAATTC G^AATTC
1334 GAATTC
FbaI 1185 TGATCA T^GATCA
1344 TGATCA
Fnu4HI 1225 GCAGC GC^[ACGT]GC
1429 GCAGC
FokI 1087 CATCC GGATG(9/13)
1467 GGATG
HhaI 1274 GCGC GCG^C
1398 GCGC
HinP1I 1274 GCGC G^CGC
1398 GCGC
HindIII 707 AAGCTT A^AGCTT
1100 AAGCTT
ItaI 1225 GCAGC GC^[ACGT]GC
1429 GCAGC
MaeIII 148 GTGAC ^GT[ACGT]AC
1495 GTGAC
MflI 371 AGATCT [GA]^GATC[CT]
1130 GGATCT
MluNI 76 TGGCCA TGG^CCA
332 TGGCCA
MscI 76 TGGCCA TGG^CCA
332 TGGCCA
MspA1I 400 CAGCTG C[AC]G^C[GT]G
1226 CAGCTG
MwoI 163 GCCTCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1216 GCCAAAGGAGC
PvuII 400 CAGCTG CAG^CTG
1226 CAGCTG
SacI 124 GAGCTC GAGCT^C
885 GAGCTC
SfcI 652 CTACAG C^T[GA][CT]AG
699 CTATAG
TaqI 1368 TCGA T^CGA
1385 TCGA
TseI 1225 GCAGC G^C[AT]GC
1429 GCAGC
Tsp45I 148 GTGAC ^GT[GC]AC
1495 GTGAC
TthHB8I 1368 TCGA T^CGA
1385 TCGA
XhoII 371 AGATCT [GA]^GATC[CT]
1130 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BmyI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI DdeI
DpnII EcoRII HaeIII HinfI HphI
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI TfiI Tru9I Tsp509I TspRI