Restriction map for
pFN21AE9538
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 414 CCGC CCGC(-3/-1)
AcsI 860 GAATTT [GA]^AATT[CT]
ApoI 860 GAATTT [GA]^AATT[CT]
AspHI 779 GAGCAC G[AT]GC[AT]^C
AspI 93 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 855 CCTAGG C^CTAGG
BamHI 491 GGATCC G^GATCC
BbsI 789 GAAGAC GAAGAC(2/6)
BclI 401 TGATCA T^GATCA
BlnI 855 CCTAGG C^CTAGG
BmyI 779 GAGCAC G[AGT]GC[ACT]^C
BpmI 326 CTGGAG CTGGAG(16/14)
BpuAI 789 GAAGAC GAAGAC(2/6)
BsiHKAI 779 GAGCAC G[AT]GC[AT]^C
BsmAI 268 GAGAC GTCTC(1/5)
Bsp1286I 779 GAGCAC G[AGT]GC[ACT]^C
BspHI 389 TCATGA T^CATGA
BsrBI 414 CCGCTC CCGCTC(-3/-3)
BsrDI 689 GCAATG GCAATG(2/0)
BssSI 429 CACGAG CACGAG(-5/-1)
BstAPI 4 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 257 CCAACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 939 TTTAAA TTT^AAA
DsaI 473 CCATGG C^C[GA][CT]GG
EarI 655 CTCTTC CTCTTC(1/4)
EcoNI 723 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 401 TGATCA T^GATCA
HindIII 361 AAGCTT A^AGCTT
Ksp632I 655 CTCTTC CTCTTC(1/4)
MspA1I 648 CAGCTG C[AC]G^C[GT]G
NcoI 473 CCATGG C^CATGG
PflFI 93 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 286 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 714 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 597 CTGCAG CTGCA^G
PvuII 648 CAGCTG CAG^CTG
RcaI 389 TCATGA T^CATGA
SfcI 597 CTGCAG C^T[GA][CT]AG
SmlI 219 CTCAAG C^T[CT][GA]AG
Tsp45I 115 GTGAC ^GT[GC]AC
Tsp509I 861 AATT ^AATT
Tth111I 93 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 286 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BbvI 231 GCTGC GCAGC(8/12)
650 GCTGC
BsrI 288 ACTGG ACTGG(1/-1)
907 CCAGT
BstYI 107 AGATCC [GA]^GATC[CT]
491 GGATCC
EcoT14I 473 CCATGG C^C[AT][AT]GG
855 CCTAGG
Fnu4HI 231 GCTGC GC^[ACGT]GC
650 GCTGC
ItaI 231 GCTGC GC^[ACGT]GC
650 GCTGC
MflI 107 AGATCC [GA]^GATC[CT]
491 GGATCC
MslI 390 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
608 CACAATGGTG
MwoI 4 GCAAACAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
407 GCCTGGACCGC
PpuMI 719 GGGTCCT [GA]G^G[AT]CC[CT]
731 AGGACCC
StyI 473 CCATGG C^C[AT][AT]GG
855 CCTAGG
TseI 231 GCTGC G^C[AT]GC
650 GCTGC
XcmI 315 CCAATGGTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
473 CCATGGTTGGCCTGG
XhoII 107 AGATCC [GA]^GATC[CT]
491 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AlwI AvaII BfaI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FokI HaeIII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TspRI