Restriction map for
pFN21AB5621
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# NO CUT ENZYMES ( 71 enzymes) #
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Acc65I AccI AclI Aor51HI AscI
Asp700I Asp718I AviII AvrII BbeI
BbvCI BcgI BlnI BsaBI BsiEI
BsiWI BspDI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstZ17I ClaI
DrdI EagI EclXI Eco47III Eco52I
EcoNI FseI FspI HgaI HpaI
KasI KpnI KspI MamI MfeI
MluI MspA1I MunI NarI NcoI
NdeI NheI NotI PacI PaeR7I
PmeI PshAI Psp1406I PvuI PvuII
RcaI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 3259 GACGTC GACGT^C
AccII 3062 CGCG CG^CG
AccIII 3919 TCCGGA T^CCGGA
AcyI 3259 GACGTC G[GA]^CG[CT]C
AflII 1885 CTTAAG C^TTAAG
AgeI 4006 ACCGGT A^CCGGT
Alw44I 1710 GTGCAC G^TGCAC
ApaI 3377 GGGCCC GGGCC^C
ApaLI 1710 GTGCAC G^TGCAC
AseI 610 ATTAAT AT^TAAT
AsnI 610 ATTAAT AT^TAAT
AspI 1915 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2535 TGGCCA TGG^CCA
BamHI 2418 GGATCC G^GATCC
BbrPI 1636 CACGTG CAC^GTG
BfrI 1885 CTTAAG C^TTAAG
BglI 2721 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1636 CACGTG [CT]AC^GT[GA]
BsaHI 3259 GACGTC G[GA]^CG[CT]C
BseAI 3919 TCCGGA T^CCGGA
BsmBI 689 CGTCTC CGTCTC(1/5)
BspEI 3919 TCCGGA T^CCGGA
BspLU11I 1330 ACATGT A^CATGT
BspMI 2206 ACCTGC ACCTGC(4/8)
BsrDI 1957 CATTGC GCAATG(2/0)
BstBI 792 TTCGAA TT^CGAA
BstUI 3062 CGCG CG^CG
BstXI 2408 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 2359 CGGACCG CG^G[AT]CCG
EcoT22I 1892 ATGCAT ATGCA^T
HaeII 2882 GGCGCT [GA]GCGC^[CT]
Hin1I 3259 GACGTC G[GA]^CG[CT]C
MluNI 2535 TGGCCA TGG^CCA
MroI 3919 TCCGGA T^CCGGA
MscI 2535 TGGCCA TGG^CCA
MvnI 3062 CGCG CG^CG
NaeI 3692 GCCGGC GCC^GGC
NgoMIV 3692 GCCGGC G^CCGGC
NruI 3061 TCGCGA TCG^CGA
NsiI 1892 ATGCAT ATGCA^T
NspV 792 TTCGAA TT^CGAA
PflFI 1915 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4006 ACCGGT A^CCGGT
PmaCI 1636 CACGTG CAC^GTG
PmlI 1636 CACGTG CAC^GTG
PshBI 610 ATTAAT AT^TAAT
RsrII 2359 CGGACCG CG^G[AT]CCG
SapI 3799 GAAGAGC GCTCTTC(1/4)
SfuI 792 TTCGAA TT^CGAA
SphI 2215 GCATGC GCATG^C
SspI 3291 AATATT AAT^ATT
StuI 2196 AGGCCT AGG^CCT
Tth111I 1915 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AflIII 473 ACGTGT A^C[GA][CT]GT
1330 ACATGT
AhdI 1641 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACGTCTGGTC
AlwNI 1765 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
3664 CAGTATCTG
AocI 2781 CCTGAGG CC^T[ACGT]AGG
3299 CCTTAGG
AspEI 1641 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACGTCTGGTC
BciVI 1818 GGATAC GTATCC(6/5)
3071 GTATCC
BclI 572 TGATCA T^GATCA
1874 TGATCA
BsgI 234 GTGCAG GTGCAG(16/14)
920 GTGCAG
BsmI 1836 GAATGC GAATGC(1/-1)
1890 GAATGC
BstEII 1066 GGTTACC G^GT[ACGT]ACC
2677 GGTCACC
BstPI 1066 GGTTACC G^GT[ACGT]ACC
2677 GGTCACC
Bsu36I 2781 CCTGAGG CC^T[ACGT]AGG
3299 CCTTAGG
CfoI 1397 GCGC GCG^C
2883 GCGC
DraI 2101 TTTAAA TTT^AAA
3978 TTTAAA
DraIII 479 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2624 CACACAGTG
DsaI 1025 CCACGG C^C[GA][CT]GG
2523 CCGTGG
Eam1105I 1641 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3259 GACGTCTGGTC
Eco81I 2781 CCTGAGG CC^T[ACGT]AGG
3299 CCTTAGG
EcoO65I 1066 GGTTACC G^GT[ACGT]ACC
2677 GGTCACC
EcoRI 2482 GAATTC G^AATTC
2749 GAATTC
FbaI 572 TGATCA T^GATCA
1874 TGATCA
HhaI 1397 GCGC GCG^C
2883 GCGC
HinP1I 1397 GCGC G^CGC
2883 GCGC
HincII 1597 GTCAAC GT[CT]^[GA]AC
2695 GTCAAC
HindII 1597 GTCAAC GT[CT]^[GA]AC
2695 GTCAAC
HindIII 721 AAGCTT A^AGCTT
1753 AAGCTT
PpuMI 1647 GGGTCCT [GA]G^G[AT]CC[CT]
3080 GGGACCT
PstI 1715 CTGCAG CTGCA^G
3670 CTGCAG
SmaI 50 CCCGGG CCC^GGG
3149 CCCGGG
XmaCI 50 CCCGGG C^CCGGG
3149 CCCGGG
XmaI 50 CCCGGG C^CCGGG
3149 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII