##################################
# NO CUT ENZYMES (  229 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MboI        MboII       MfeI        MflI        
MluI        MnlI        MroI        MseI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

BalI           11  TGGCCA               TGG^CCA
BfaI          124  CTAG                 C^TAG
BsaJI           8  CCTTGG               C^C[ACGT][ACGT]GG
BsmAI          32  GTCTC                GTCTC(1/5)
BsmI           47  GCATTC               GAATGC(1/-1)
BspHI          99  TCATGA               T^CATGA
BsrI          128  CCAGT                ACTGG(1/-1)
BstXI         220  CCAAAAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         123  GCTAGC               GC[ACGT]^[ACGT]GC
EaeI           11  TGGCCA               [CT]^GGCC[GA]
EcoT14I         8  CCTTGG               C^C[AT][AT]GG
HaeIII         12  GGCC                 GG^CC
HinfI          39  GATTC                G^A[ACGT]TC
HphI           88  TCACC                GGTGA(8/7)
MaeI          124  CTAG                 C^TAG
MaeIII        214  GTTAC                ^GT[ACGT]AC
MluNI          11  TGGCCA               TGG^CCA
MscI           11  TGGCCA               TGG^CCA
MslI           70  CATAGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          123  GCTAGC               G^CTAGC
NlaIV         191  GGAACC               GG[ACGT]^[ACGT]CC
RcaI           99  TCATGA               T^CATGA
SmlI           34  CTCAAG               C^T[CT][GA]AG
StyI            8  CCTTGG               C^C[AT][AT]GG
TfiI           39  GATTC                G^A[AT]TC
TspRI         129  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

DdeI           25  CTAAG                C^T[ACGT]AG
              173  CTCAG               
MwoI           13  GCCAGAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              118  GCTGTGCTAGC         


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

NlaIII