Restriction map for
pFN21AE3305
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccII AccIII AcyI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BcnI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FseI
FspI HaeII HapII Hin1I HpaII
KasI KspI MamI MluI MluNI
MroI MscI MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1980 GGTACC G^GTACC
AclI 1087 AACGTT AA^CGTT
AflIII 1727 ACATGT A^C[GA][CT]GT
AhdI 561 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1980 GGTACC G^GTACC
AspEI 561 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 2311 GGGCTC G[GA]GC[CT]^C
BcgI 1196 CGAAGGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 378 AGATCT A^GATCT
BlpI 193 GCTGAGC GC^T[ACGT]AGC
BmyI 2311 GGGCTC G[AGT]GC[ACT]^C
BpmI 1918 CTCCAG CTGGAG(16/14)
Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC
BsgI 1269 CTGCAC GTGCAG(16/14)
Bsp1286I 2311 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 971 TGTACA T^GTACA
BspLU11I 1727 ACATGT A^CATGT
BsrGI 971 TGTACA T^GTACA
Cac8I 1661 GCTGGC GC[ACGT]^[ACGT]GC
CelII 193 GCTGAGC GC^T[ACGT]AGC
CfoI 1875 GCGC GCG^C
DraII 401 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 561 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2079 GAAGAG CTCTTC(1/4)
EcoO109I 401 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1236 GAATTC G^AATTC
EcoRV 342 GATATC GAT^ATC
EcoT14I 2291 CCAAGG C^C[AT][AT]GG
HgaI 2265 GACGC GACGC(5/10)
HhaI 1875 GCGC GCG^C
HinP1I 1875 GCGC G^CGC
HpaI 1005 GTTAAC GTT^AAC
KpnI 1980 GGTACC GGTAC^C
Ksp632I 2079 GAAGAG CTCTTC(1/4)
MfeI 494 CAATTG C^AATTG
MunI 494 CAATTG C^AATTG
PshAI 697 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1087 AACGTT AA^CGTT
SspBI 971 TGTACA T^GTACA
StyI 2291 CCAAGG C^C[AT][AT]GG
XcmI 1781 CCATGCTGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaII 742 GGTCC G^G[AT]CC
1862 GGACC
BbsI 1305 GTCTTC GAAGAC(2/6)
2015 GTCTTC
BpuAI 1305 GTCTTC GAAGAC(2/6)
2015 GTCTTC
BsaI 204 GGTCTC GGTCTC(1/5)
1186 GGTCTC
BsmFI 1580 GGGAC GGGAC(10/14)
2035 GTCCC
BstXI 824 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCACCATGCTGG
HincII 231 GTTGAC GT[CT]^[GA]AC
1005 GTTAAC
HindII 231 GTTGAC GT[CT]^[GA]AC
1005 GTTAAC
HindIII 30 AAGCTT A^AGCTT
1418 AAGCTT
MspA1I 151 CCGCTG C[AC]G^C[GT]G
2269 CAGCGG
MwoI 1475 GCAGCTTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1478 GCTTTAGCAGC
NspI 1034 ACATGC [GA]CATG^[CT]
1727 ACATGT
SfaNI 507 GCATC GCATC(5/9)
1817 GCATC
SpeI 616 ACTAGT A^CTAGT
2358 ACTAGT
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
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AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I BanI BbvI
BclI BfaI BsaJI BsiYI BslI
BsmAI BsmI BspHI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI