##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HapII       
HgaI        Hin1I       HindIII     HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI          431  GTCGAC               GT^[AC][GT]AC
AcsI          629  AAATTC               [GA]^AATT[CT]
ApoI          629  AAATTC               [GA]^AATT[CT]
AseI          778  ATTAAT               AT^TAAT
AsnI          778  ATTAAT               AT^TAAT
AvaI          178  CCCGAG               C^[CT]CG[GA]G
BamHI         240  GGATCC               G^GATCC
BanI          112  GGCACC               G^G[CT][GA]CC
BbsI          481  GAAGAC               GAAGAC(2/6)
BglII         668  AGATCT               A^GATCT
BmyI          111  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         481  GAAGAC               GAAGAC(2/6)
BsgI          198  CTGCAC               GTGCAG(16/14)
BsoBI         178  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      111  GGGCAC               G[AGT]GC[ACT]^C
BstAPI         48  GCAGCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI          220  TTTAAA               TTT^AAA
DraII         693  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoNI         639  CCTCCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      693  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       539  CCAAGG               C^C[AT][AT]GG
EcoT22I       679  ATGCAT               ATGCA^T
HaeII         228  GGCGCT               [GA]GCGC^[CT]
HpaI          346  GTTAAC               GTT^AAC
HphI          555  GGTGA                GGTGA(8/7)
MaeII         563  ACGT                 A^CGT
NsiI          679  ATGCAT               ATGCA^T
PpuMI         693  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         778  ATTAAT               AT^TAAT
PstI           46  CTGCAG               CTGCA^G
SalI          431  GTCGAC               G^TCGAC
SfcI           46  CTGCAG               C^T[GA][CT]AG
SmlI          420  CTTGAG               C^T[CT][GA]AG
StyI          539  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          159  GTAC                 GT^AC
              768  GTAC                
AlwNI          37  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
               49  CAGCTTCTG           
AvaII         694  GGACC                G^G[AT]CC
              816  GGACC               
BpmI           34  CTCCAG               CTGGAG(16/14)
              640  CTCCAG              
BstYI         240  GGATCC               [GA]^GATC[CT]
              668  AGATCT              
HaeIII          6  GGCC                 GG^CC
              145  GGCC                
HincII        346  GTTAAC               GT[CT]^[GA]AC
              431  GTCGAC              
HindII        346  GTTAAC               GT[CT]^[GA]AC
              431  GTCGAC              
MboII         481  GAAGA                GAAGA(8/7)
              137  TCTTC               
MflI          240  GGATCC               [GA]^GATC[CT]
              668  AGATCT              
PvuII          37  CAGCTG               CAG^CTG
              771  CAGCTG              
RsaI          159  GTAC                 GT^AC
              768  GTAC                
SfaNI          22  GCATC                GCATC(5/9)
              523  GCATC               
XhoII         240  GGATCC               [GA]^GATC[CT]
              668  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbvI        
BsaJI       BsiYI       BslI        BsmFI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      FauI        Fnu4HI      FokI        HhaI        
HinP1I      HinfI       ItaI        MboI        MnlI        
MseI        MspA1I      MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I