Restriction map for
pFN21AE2696
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
AspEI AspI AvaI AvrII BamHI
BanII BbeI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI HincII HindII HindIII HpaI
HphI KasI Ksp632I MaeIII MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TfiI Tsp45I Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 118 GACGTC GACGT^C
Acc65I 305 GGTACC G^GTACC
AccI 397 GTCTAC GT^[AC][GT]AC
AcyI 118 GACGTC G[GA]^CG[CT]C
AfaI 306 GTAC GT^AC
Alw44I 592 GTGCAC G^TGCAC
ApaLI 592 GTGCAC G^TGCAC
Asp718I 305 GGTACC G^GTACC
AspHI 592 GTGCAC G[AT]GC[AT]^C
AviII 185 TGCGCA TGC^GCA
BalI 331 TGGCCA TGG^CCA
BanI 305 GGTACC G^G[CT][GA]CC
BbrPI 589 CACGTG CAC^GTG
BbsI 354 GAAGAC GAAGAC(2/6)
BbvCI 309 CCTCAGC CCTCAGC(-5/-2)
BlpI 219 GCTCAGC GC^T[ACGT]AGC
BmyI 592 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 219 GCTCAGC GC^T[ACGT]AGC
BpuAI 354 GAAGAC GAAGAC(2/6)
BsaAI 589 CACGTG [CT]AC^GT[GA]
BsaHI 118 GACGTC G[GA]^CG[CT]C
BsaI 440 GAGACC GGTCTC(1/5)
BsiHKAI 592 GTGCAC G[AT]GC[AT]^C
BsmAI 440 GAGAC GTCTC(1/5)
Bsp1286I 592 GTGCAC G[AGT]GC[ACT]^C
BspHI 867 TCATGA T^CATGA
BssHII 201 GCGCGC G^CGCGC
BstBI 729 TTCGAA TT^CGAA
BstYI 680 AGATCC [GA]^GATC[CT]
CelII 219 GCTCAGC GC^T[ACGT]AGC
DraIII 637 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 752 CCAAGG C^C[AT][AT]GG
FspI 185 TGCGCA TGC^GCA
Hin1I 118 GACGTC G[GA]^CG[CT]C
KpnI 305 GGTACC GGTAC^C
KspI 245 CCGCGG CCGC^GG
MflI 680 AGATCC [GA]^GATC[CT]
MluNI 331 TGGCCA TGG^CCA
MscI 331 TGGCCA TGG^CCA
MseI 743 TTAA T^TAA
NotI 241 GCGGCCGC GC^GGCCGC
NspV 729 TTCGAA TT^CGAA
PmaCI 589 CACGTG CAC^GTG
PmlI 589 CACGTG CAC^GTG
RcaI 867 TCATGA T^CATGA
RsaI 306 GTAC GT^AC
SacII 245 CCGCGG CCGC^GG
SfcI 399 CTACAG C^T[GA][CT]AG
SfuI 729 TTCGAA TT^CGAA
SspI 860 AATATT AAT^ATT
StyI 752 CCAAGG C^C[AT][AT]GG
Tru9I 743 TTAA T^TAA
XhoII 680 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 681 GATCC GGATC(4/5)
832 GATCC
Asp700I 859 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
882 GAAGTTCTTC
BcnI 783 CCGGG CC^[GC]GG
813 CCGGG
BfaI 49 CTAG C^TAG
73 CTAG
BpmI 109 CTCCAG CTGGAG(16/14)
211 CTGGAG
BseRI 277 GAGGAG GAGGAG(10/8)
523 GAGGAG
BsgI 226 CTGCAC GTGCAG(16/14)
853 CTGCAC
DsaI 245 CCGCGG C^C[GA][CT]GG
291 CCACGG
EagI 242 CGGCCG C^GGCCG
780 CGGCCG
EclXI 242 CGGCCG C^GGCCG
780 CGGCCG
Eco52I 242 CGGCCG C^GGCCG
780 CGGCCG
HinfI 395 GAGTC G^A[ACGT]TC
802 GAGTC
MaeI 49 CTAG C^TAG
73 CTAG
MslI 177 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
471 CACCCAAGTG
NciI 783 CCGGG CC^[GC]GG
813 CCGGG
NspI 491 ACATGC [GA]CATG^[CT]
596 ACATGC
PflMI 291 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
636 CCACATTGTGG
PleI 395 GAGTC GAGTC(4/5)
802 GAGTC
PpuMI 103 GGGACCC [GA]G^G[AT]CC[CT]
665 GGGACCC
SfaNI 216 GATGC GCATC(5/9)
318 GATGC
TspRI 206 CACTG CA[GC]TG[ACGT][ACGT]^
347 CAGTG
Van91I 291 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
636 CCACATTGTGG
XmnI 859 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
882 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BsaJI BsiEI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
TthHB8I