Restriction map for
pFN21AE1902
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI DsaI EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 421 GACGTC GACGT^C
AfaI 69 GTAC GT^AC
AflIII 488 ACATGT A^C[GA][CT]GT
AlwI 747 GATCC GGATC(4/5)
AocI 1260 CCTCAGG CC^T[ACGT]AGG
Aor51HI 683 AGCGCT AGC^GCT
AspHI 1255 GAGCTC G[AT]GC[AT]^C
AspI 1134 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 726 TGCGCA TGC^GCA
BbeI 250 GGCGCC GGCGC^C
BbsI 661 GAAGAC GAAGAC(2/6)
BglII 779 AGATCT A^GATCT
BlpI 333 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 333 GCTGAGC GC^T[ACGT]AGC
BpuAI 661 GAAGAC GAAGAC(2/6)
BsaAI 709 TACGTG [CT]AC^GT[GA]
BsaI 448 GAGACC GGTCTC(1/5)
BsiHKAI 1255 GAGCTC G[AT]GC[AT]^C
BspLU11I 488 ACATGT A^CATGT
BspMI 207 GCAGGT ACCTGC(4/8)
BsrFI 1045 GCCGGC [GA]^CCGG[CT]
BstAPI 324 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1260 CCTCAGG CC^T[ACGT]AGG
CelII 333 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1045 GCCGGC [GA]^CCGG[CT]
DraIII 1006 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1047 CGGCCG C^GGCCG
EclXI 1047 CGGCCG C^GGCCG
Eco47III 683 AGCGCT AGC^GCT
Eco52I 1047 CGGCCG C^GGCCG
Eco81I 1260 CCTCAGG CC^T[ACGT]AGG
EcoT14I 437 CCTTGG C^C[AT][AT]GG
FspI 726 TGCGCA TGC^GCA
HphI 648 GGTGA GGTGA(8/7)
KasI 250 GGCGCC G^GCGCC
MseI 637 TTAA T^TAA
MslI 574 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1045 GCCGGC GCC^GGC
NarI 250 GGCGCC GG^CGCC
NgoMIV 1045 GCCGGC G^CCGGC
PaeR7I 1031 CTCGAG C^TCGAG
PflFI 1134 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 651 GACTC GAGTC(4/5)
PvuI 945 CGATCG CGAT^CG
RsaI 69 GTAC GT^AC
SacI 1255 GAGCTC GAGCT^C
SapI 1252 GAAGAGC GCTCTTC(1/4)
SbfI 99 CCTGCAGG CCTGCA^GG
SexAI 1007 ACCAGGT A^CC[AT]GGT
SfoI 250 GGCGCC GGC^GCC
SmlI 1031 CTCGAG C^T[CT][GA]AG
SphI 528 GCATGC GCATG^C
Sse8387I 99 CCTGCAGG CCTGCA^GG
StuI 1126 AGGCCT AGG^CCT
StyI 437 CCTTGG C^C[AT][AT]GG
Tru9I 637 TTAA T^TAA
Tsp509I 279 AATT ^AATT
Tth111I 1134 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1031 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 10 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACTTCATGTC
AspEI 10 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACTTCATGTC
BalI 440 TGGCCA TGG^CCA
503 TGGCCA
BanI 250 GGCGCC G^G[CT][GA]CC
1110 GGTGCC
BfaI 187 CTAG C^TAG
301 CTAG
BglI 148 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
253 GCCGCCGAGGC
BsiEI 945 CGATCG CG[GA][CT]^CG
1047 CGGCCG
BsmAI 448 GAGAC GTCTC(1/5)
1020 GAGAC
BsrBI 432 CCGCTC CCGCTC(-3/-3)
880 GAGCGG
BstXI 506 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCAAGTATTTGG
BstYI 746 AGATCC [GA]^GATC[CT]
779 AGATCT
Eam1105I 10 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACTTCATGTC
EarI 805 GAAGAG CTCTTC(1/4)
1252 GAAGAG
HaeII 250 GGCGCC [GA]GCGC^[CT]
683 AGCGCT
Ksp632I 805 GAAGAG CTCTTC(1/4)
1252 GAAGAG
MaeI 187 CTAG C^TAG
301 CTAG
MflI 746 AGATCC [GA]^GATC[CT]
779 AGATCT
MluNI 440 TGGCCA TGG^CCA
503 TGGCCA
MscI 440 TGGCCA TGG^CCA
503 TGGCCA
TaqI 630 TCGA T^CGA
1032 TCGA
TthHB8I 630 TCGA T^CGA
1032 TCGA
XhoII 746 AGATCC [GA]^GATC[CT]
779 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TfiI TseI
Tsp45I TspRI XmaCI XmaI