Restriction map for
pFN21AE9684
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# NO CUT ENZYMES ( 80 enzymes) #
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AclI AflII AflIII AgeI Aor51HI
AscI AseI AsnI AvrII BbrPI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HincII HindII HpaI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SgrAI
SmaI SnaBI SpeI SspBI SspI
StuI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1778 GACGTC GACGT^C
Acc65I 2288 GGTACC G^GTACC
AccI 1715 GTAGAC GT^[AC][GT]AC
AccIII 2354 TCCGGA T^CCGGA
Alw44I 878 GTGCAC G^TGCAC
ApaI 1872 GGGCCC GGGCC^C
ApaLI 878 GTGCAC G^TGCAC
Asp700I 2157 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2288 GGTACC G^GTACC
AspI 621 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2414 TGCGCA TGC^GCA
BamHI 186 GGATCC G^GATCC
BbeI 1033 GGCGCC GGCGC^C
BglII 2139 AGATCT A^GATCT
BsaBI 2412 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2354 TCCGGA T^CCGGA
BsgI 1423 GTGCAG GTGCAG(16/14)
BspEI 2354 TCCGGA T^CCGGA
BsrDI 35 GCAATG GCAATG(2/0)
BsrFI 707 GCCGGC [GA]^CCGG[CT]
BstAPI 795 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2035 TTCGAA TT^CGAA
Cfr10I 707 GCCGGC [GA]^CCGG[CT]
DraIII 919 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 22 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 347 GAATTC G^AATTC
FspI 2414 TGCGCA TGC^GCA
HaeII 1033 GGCGCC [GA]GCGC^[CT]
HgaI 195 GACGC GACGC(5/10)
HindIII 1795 AAGCTT A^AGCTT
KasI 1033 GGCGCC G^GCGCC
KpnI 2288 GGTACC GGTAC^C
KspI 1783 CCGCGG CCGC^GG
MaeII 1779 ACGT A^CGT
MamI 2412 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2354 TCCGGA T^CCGGA
NaeI 707 GCCGGC GCC^GGC
NarI 1033 GGCGCC GG^CGCC
NgoMIV 707 GCCGGC G^CCGGC
NspV 2035 TTCGAA TT^CGAA
PaeR7I 990 CTCGAG C^TCGAG
PflFI 621 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1990 GAGCTC GAGCT^C
SacII 1783 CCGCGG CCGC^GG
SapI 467 GCTCTTC GCTCTTC(1/4)
SbfI 792 CCTGCAGG CCTGCA^GG
SexAI 920 ACCTGGT A^CC[AT]GGT
SfiI 2382 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1033 GGCGCC GGC^GCC
SfuI 2035 TTCGAA TT^CGAA
Sse8387I 792 CCTGCAGG CCTGCA^GG
TfiI 204 GATTC G^A[AT]TC
Tth111I 621 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 990 CTCGAG C^TCGAG
XmnI 2157 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 347 GAATTC [GA]^AATT[CT]
1015 GAATTT
AcyI 1033 GGCGCC G[GA]^CG[CT]C
1778 GACGTC
AhdI 942 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACACCAAGTC
ApoI 347 GAATTC [GA]^AATT[CT]
1015 GAATTT
AspEI 942 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACACCAAGTC
AvaI 990 CTCGAG C^[CT]CG[GA]G
1196 CTCGGG
BcnI 282 CCCGG CC^[GC]GG
1965 CCGGG
BglI 2296 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2383 GCCCTCCAGGC
BsaHI 1033 GGCGCC G[GA]^CG[CT]C
1778 GACGTC
BsoBI 990 CTCGAG C^[CT]CG[GA]G
1196 CTCGGG
Eam1105I 942 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1261 GACACCAAGTC
EcoNI 1834 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2397 CCTGAGGAAGG
Hin1I 1033 GGCGCC G[GA]^CG[CT]C
1778 GACGTC
MseI 16 TTAA T^TAA
1646 TTAA
NciI 282 CCCGG CC^[GC]GG
1965 CCGGG
NcoI 2057 CCATGG C^CATGG
2150 CCATGG
NspI 1341 GCATGC [GA]CATG^[CT]
1748 GCATGC
PvuII 1387 CAGCTG CAG^CTG
2326 CAGCTG
SphI 1341 GCATGC GCATG^C
1748 GCATGC
Tru9I 16 TTAA T^TAA
1646 TTAA
XcmI 914 CCAACCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
918 CCACCTGGTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII