Restriction map for
pFN21AE4031
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspHI AspI AvaI
AviII AvrII BalI BanII BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BmyI BpuAI
BsaAI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI Ksp632I MaeII MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StyI
SwaI TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AhdI 90 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 114 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 555 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 90 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 636 GGACC G^G[AT]CC
BamHI 518 GGATCC G^GATCC
BbeI 684 GGCGCC GGCGC^C
BcgI 539 CGACCGTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 313 CCGGG CC^[GC]GG
BfaI 665 CTAG C^TAG
BlpI 527 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 527 GCTCAGC GC^T[ACGT]AGC
BsaI 331 GAGACC GGTCTC(1/5)
BsiEI 539 CGACCG CG[GA][CT]^CG
BsmFI 26 GGGAC GGGAC(10/14)
BspHI 207 TCATGA T^CATGA
BsrBI 688 CCGCTC CCGCTC(-3/-3)
BstAPI 113 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 87 GGTGACC G^GT[ACGT]ACC
BstF5I 587 CATCC GGATG(2/0)
BstPI 87 GGTGACC G^GT[ACGT]ACC
BstXI 363 CCATCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 500 GCGGGC GC[ACGT]^[ACGT]GC
CelII 527 GCTCAGC GC^T[ACGT]AGC
Cfr13I 636 GGACC G^G[ACGT]CC
DraIII 663 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 90 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 87 GGTGACC G^GT[ACGT]ACC
FokI 587 CATCC GGATG(9/13)
HaeII 684 GGCGCC [GA]GCGC^[CT]
HgaI 76 GACGC GACGC(5/10)
HincII 3 GTTGAC GT[CT]^[GA]AC
HindII 3 GTTGAC GT[CT]^[GA]AC
KasI 684 GGCGCC G^GCGCC
KspI 410 CCGCGG CCGC^GG
MaeI 665 CTAG C^TAG
MseI 593 TTAA T^TAA
NarI 684 GGCGCC GG^CGCC
NciI 313 CCGGG CC^[GC]GG
RcaI 207 TCATGA T^CATGA
SacII 410 CCGCGG CCGC^GG
Sau96I 636 GGACC G^G[ACGT]CC
SbfI 161 CCTGCAGG CCTGCA^GG
SfoI 684 GGCGCC GGC^GCC
SmlI 646 CTCAAG C^T[CT][GA]AG
Sse8387I 161 CCTGCAGG CCTGCA^GG
StuI 249 AGGCCT AGG^CCT
TaqI 74 TCGA T^CGA
Tru9I 593 TTAA T^TAA
TthHB8I 74 TCGA T^CGA
XmnI 555 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 65 GGTACC G^GTACC
243 GGTACC
AccII 411 CGCG CG^CG
499 CGCG
AcyI 76 GACGCC G[GA]^CG[CT]C
684 GGCGCC
Asp718I 65 GGTACC G^GTACC
243 GGTACC
BpmI 136 CTGGAG CTGGAG(16/14)
475 CTGGAG
BsaBI 168 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
358 GATTCCCATC
BsaHI 76 GACGCC G[GA]^CG[CT]C
684 GGCGCC
BsmAI 317 GAGAC GTCTC(1/5)
331 GAGAC
BstUI 411 CGCG CG^CG
499 CGCG
BstYI 518 GGATCC [GA]^GATC[CT]
623 AGATCC
CfoI 498 GCGC GCG^C
685 GCGC
DdeI 354 CTTAG C^T[ACGT]AG
528 CTCAG
DsaI 83 CCACGG C^C[GA][CT]GG
410 CCGCGG
EcoNI 107 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
627 CCTGGGCAAGG
FauI 62 GCGGG CCCGC(4/6)
500 GCGGG
HaeIII 250 GGCC GG^CC
408 GGCC
HapII 313 CCGG C^CGG
424 CCGG
HhaI 498 GCGC GCG^C
685 GCGC
Hin1I 76 GACGCC G[GA]^CG[CT]C
684 GGCGCC
HinP1I 498 GCGC G^CGC
685 GCGC
HinfI 358 GATTC G^A[ACGT]TC
674 GATTC
HpaII 313 CCGG C^CGG
424 CCGG
KpnI 65 GGTACC GGTAC^C
243 GGTACC
MamI 168 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
358 GATTCCCATC
MboII 12 GAAGA GAAGA(8/7)
555 GAAGA
MflI 518 GGATCC [GA]^GATC[CT]
623 AGATCC
MspI 313 CCGG C^CGG
424 CCGG
MvnI 411 CGCG CG^CG
499 CGCG
SfaNI 467 GCATC GCATC(5/9)
549 GCATC
TfiI 358 GATTC G^A[AT]TC
674 GATTC
Tsp45I 88 GTGAC ^GT[GC]AC
661 GTCAC
Tsp509I 239 AATT ^AATT
296 AATT
XhoII 518 GGATCC [GA]^GATC[CT]
623 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BbvI BsaJI BseRI BsgI BsiYI
BslI BspMI BssKI BstNI DpnII
EcoRII Fnu4HI HphI ItaI MaeIII
MboI MnlI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PstI
RsaI Sau3AI ScrFI SfcI TseI