Restriction map for
pFN21AE4305
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccIII AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI Ksp632I MamI MfeI MluI
MroI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 901 GTAGAC GT^[AC][GT]AC
AccII 837 CGCG CG^CG
AclI 644 AACGTT AA^CGTT
AflII 150 CTTAAG C^TTAAG
AlwI 216 GGATC GGATC(4/5)
AspHI 327 GAGCAC G[AT]GC[AT]^C
AvaI 443 CTCGAG C^[CT]CG[GA]G
BbsI 132 GAAGAC GAAGAC(2/6)
BciVI 680 GTATCC GTATCC(6/5)
BfaI 472 CTAG C^TAG
BfrI 150 CTTAAG C^TTAAG
BmyI 327 GAGCAC G[AGT]GC[ACT]^C
BpuAI 132 GAAGAC GAAGAC(2/6)
BsaI 725 GGTCTC GGTCTC(1/5)
BsiHKAI 327 GAGCAC G[AT]GC[AT]^C
BsmBI 880 GAGACG CGTCTC(1/5)
BsoBI 443 CTCGAG C^[CT]CG[GA]G
Bsp1286I 327 GAGCAC G[AGT]GC[ACT]^C
BsrDI 109 CATTGC GCAATG(2/0)
BsrI 97 CCAGT ACTGG(1/-1)
BstAPI 329 GCACAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 837 CGCG CG^CG
BstYI 216 GGATCT [GA]^GATC[CT]
DpnII 217 GATC ^GATC
DraI 778 TTTAAA TTT^AAA
DraII 10 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 66 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 10 AGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 359 GCGTC GACGC(5/10)
KspI 836 CCGCGG CCGC^GG
MaeI 472 CTAG C^TAG
MboI 217 GATC ^GATC
MflI 216 GGATCT [GA]^GATC[CT]
MspA1I 836 CCGCGG C[AC]G^C[GT]G
MvnI 837 CGCG CG^CG
NcoI 848 CCATGG C^CATGG
NdeII 217 GATC ^GATC
PaeR7I 443 CTCGAG C^TCGAG
PleI 313 GACTC GAGTC(4/5)
PpuMI 10 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 644 AACGTT AA^CGTT
SacII 836 CCGCGG CCGC^GG
Sau3AI 217 GATC ^GATC
StuI 342 AGGCCT AGG^CCT
TaqI 444 TCGA T^CGA
Tsp45I 361 GTCAC ^GT[GC]AC
TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 444 TCGA T^CGA
XcmI 684 CCAATATAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 443 CTCGAG C^TCGAG
XhoII 216 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 117 GGTACC G^GTACC
592 GGTACC
AcsI 520 AAATTC [GA]^AATT[CT]
691 AAATTT
ApoI 520 AAATTC [GA]^AATT[CT]
691 AAATTT
Asp718I 117 GGTACC G^GTACC
592 GGTACC
BalI 275 TGGCCA TGG^CCA
851 TGGCCA
BanI 117 GGTACC G^G[CT][GA]CC
592 GGTACC
BsmAI 880 GAGAC GTCTC(1/5)
726 GTCTC
BsmI 303 GAATGC GAATGC(1/-1)
355 GAATGC
BstXI 147 CCACTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
186 CCATCCTGATGG
Cac8I 201 GCTTGC GC[ACGT]^[ACGT]GC
235 GCTTGC
DdeI 794 CTGAG C^T[ACGT]AG
864 CTCAG
DsaI 836 CCGCGG C^C[GA][CT]GG
848 CCATGG
EcoT14I 501 CCTTGG C^C[AT][AT]GG
848 CCATGG
FauI 143 CCCGC CCCGC(4/6)
838 GCGGG
KpnI 117 GGTACC GGTAC^C
592 GGTACC
MaeII 577 ACGT A^CGT
645 ACGT
MluNI 275 TGGCCA TGG^CCA
851 TGGCCA
MscI 275 TGGCCA TGG^CCA
851 TGGCCA
NlaIII 179 CATG CATG^
849 CATG
PstI 67 CTGCAG CTGCA^G
461 CTGCAG
SfaNI 420 GCATC GCATC(5/9)
554 GCATC
StyI 501 CCTTGG C^C[AT][AT]GG
848 CCATGG
Tsp509I 521 AATT ^AATT
692 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BsiYI BslI BspMI
BssKI BstF5I BstNI Cfr13I EaeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MseI MslI MvaI MwoI NlaIV
RsaI Sau96I ScrFI SfcI SmlI
TfiI Tru9I TseI