Restriction map for
pFN21AB6244
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MamI MluI MroI MspA1I
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 861 GGTACC G^GTACC
AccI 688 GTCTAC GT^[AC][GT]AC
AcyI 432 GGCGCC G[GA]^CG[CT]C
AflIII 533 ACGTGT A^C[GA][CT]GT
AlwNI 1702 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 658 CCTCAGG CC^T[ACGT]AGG
Asp700I 1974 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 861 GGTACC G^GTACC
BalI 322 TGGCCA TGG^CCA
BbeI 432 GGCGCC GGCGC^C
BbsI 845 GAAGAC GAAGAC(2/6)
BfaI 782 CTAG C^TAG
BglI 559 GCCAAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 845 GAAGAC GAAGAC(2/6)
BsaHI 432 GGCGCC G[GA]^CG[CT]C
BsaI 1894 GAGACC GGTCTC(1/5)
BsgI 1608 CTGCAC GTGCAG(16/14)
Bsp1407I 908 TGTACA T^GTACA
BsrFI 1811 ACCGGC [GA]^CCGG[CT]
BsrGI 908 TGTACA T^GTACA
Bsu36I 658 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1811 ACCGGC [GA]^CCGG[CT]
DraIII 17 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1739 CCATGG C^C[GA][CT]GG
EarI 1046 GAAGAG CTCTTC(1/4)
Eco81I 658 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1739 CCATGG C^C[AT][AT]GG
Hin1I 432 GGCGCC G[GA]^CG[CT]C
KasI 432 GGCGCC G^GCGCC
KpnI 861 GGTACC GGTAC^C
Ksp632I 1046 GAAGAG CTCTTC(1/4)
MaeI 782 CTAG C^TAG
MfeI 1292 CAATTG C^AATTG
MluNI 322 TGGCCA TGG^CCA
MscI 322 TGGCCA TGG^CCA
MseI 964 TTAA T^TAA
MunI 1292 CAATTG C^AATTG
NarI 432 GGCGCC GG^CGCC
NcoI 1739 CCATGG C^CATGG
NdeI 1373 CATATG CA^TATG
PflMI 1531 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 35 AGTACT AGT^ACT
SfoI 432 GGCGCC GGC^GCC
SmaI 1898 CCCGGG CCC^GGG
SphI 290 GCATGC GCATG^C
SspBI 908 TGTACA T^GTACA
StyI 1739 CCATGG C^C[AT][AT]GG
Tru9I 964 TTAA T^TAA
Van91I 1531 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1739 CCATGGATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1898 CCCGGG C^CCGGG
XmaI 1898 CCCGGG C^CCGGG
XmnI 1974 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 414 CGCG CG^CG
676 CGCG
AvaI 1898 CCCGGG C^[CT]CG[GA]G
1961 CCCGAG
BanII 343 GGGCTC G[GA]GC[CT]^C
1884 GGGCTC
BbvCI 1282 CCTCAGC CCTCAGC(-5/-2)
1450 CCTCAGC
BlpI 700 GCTGAGC GC^T[ACGT]AGC
744 GCTGAGC
BpmI 1887 CTCCAG CTGGAG(16/14)
1538 CTGGAG
Bpu1102I 700 GCTGAGC GC^T[ACGT]AGC
744 GCTGAGC
BsoBI 1898 CCCGGG C^[CT]CG[GA]G
1961 CCCGAG
BstUI 414 CGCG CG^CG
676 CGCG
CelII 700 GCTGAGC GC^T[ACGT]AGC
744 GCTGAGC
EaeI 322 TGGCCA [CT]^GGCC[GA]
566 CGGCCA
FauI 487 CCCGC CCCGC(4/6)
519 CCCGC
HaeII 432 GGCGCC [GA]GCGC^[CT]
1124 AGCGCC
HgaI 1010 GACGC GACGC(5/10)
425 GCGTC
MvnI 414 CGCG CG^CG
676 CGCG
SfcI 31 CTACAG C^T[GA][CT]AG
1871 CTACAG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BbvI
BciVI BcnI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII