Restriction map for
pFN21AE4385
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AvaI AviII BamHI BanII BbeI
BbrPI BbsI BbvCI BfrI BglI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstUI BstXI
CelII Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoT22I
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 806 GGTACC G^GTACC
AccI 781 GTATAC GT^[AC][GT]AC
AflIII 1052 ACGTGT A^C[GA][CT]GT
AlwI 571 GGATC GGATC(4/5)
AlwNI 30 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 624 CCTGAGG CC^T[ACGT]AGG
Aor51HI 722 AGCGCT AGC^GCT
Asp718I 806 GGTACC G^GTACC
AspI 616 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 319 CCTAGG C^CTAGG
BalI 161 TGGCCA TGG^CCA
BcgI 596 GCAGAGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 609 TGATCA T^GATCA
BcnI 432 CCGGG CC^[GC]GG
BglII 332 AGATCT A^GATCT
BlnI 319 CCTAGG C^CTAGG
BsmAI 239 GTCTC GTCTC(1/5)
Bsp1407I 743 TGTACA T^GTACA
BspDI 604 ATCGAT AT^CGAT
BsrGI 743 TGTACA T^GTACA
BsrI 180 CCAGT ACTGG(1/-1)
Bst1107I 781 GTATAC GTA^TAC
BstEII 708 GGTTACC G^GT[ACGT]ACC
BstPI 708 GGTTACC G^GT[ACGT]ACC
BstZ17I 781 GTATAC GTA^TAC
Bsu36I 624 CCTGAGG CC^T[ACGT]AGG
Cac8I 1087 GCCTGC GC[ACGT]^[ACGT]GC
ClaI 604 ATCGAT AT^CGAT
DraI 112 TTTAAA TTT^AAA
EaeI 161 TGGCCA [CT]^GGCC[GA]
Eco47III 722 AGCGCT AGC^GCT
Eco81I 624 CCTGAGG CC^T[ACGT]AGG
EcoNI 249 CCTAGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 708 GGTTACC G^GT[ACGT]ACC
EcoRI 130 GAATTC G^AATTC
EcoRV 793 GATATC GAT^ATC
FbaI 609 TGATCA T^GATCA
HaeII 722 AGCGCT [GA]GCGC^[CT]
HphI 1134 GGTGA GGTGA(8/7)
KpnI 806 GGTACC GGTAC^C
MaeII 1052 ACGT A^CGT
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
NciI 432 CCGGG CC^[GC]GG
PflFI 616 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 961 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 147 GAGTC GAGTC(4/5)
PvuII 30 CAGCTG CAG^CTG
SapI 299 GCTCTTC GCTCTTC(1/4)
SexAI 1007 ACCAGGT A^CC[AT]GGT
SspBI 743 TGTACA T^GTACA
Tth111I 616 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 961 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 816 TCCGGA T^CCGGA
1202 TCCGGA
AcsI 130 GAATTC [GA]^AATT[CT]
1128 GAATTT
ApoI 130 GAATTC [GA]^AATT[CT]
1128 GAATTT
BciVI 187 GTATCC GTATCC(6/5)
448 GTATCC
BsaWI 816 TCCGGA [AT]^CCGG[AT]
1202 TCCGGA
BseAI 816 TCCGGA T^CCGGA
1202 TCCGGA
BspEI 816 TCCGGA T^CCGGA
1202 TCCGGA
BspHI 612 TCATGA T^CATGA
989 TCATGA
BspMI 1172 ACCTGC ACCTGC(4/8)
545 GCAGGT
BstYI 332 AGATCT [GA]^GATC[CT]
571 GGATCT
DsaI 164 CCATGG C^C[GA][CT]GG
452 CCATGG
EarI 300 CTCTTC CTCTTC(1/4)
940 GAAGAG
FauI 1110 CCCGC CCCGC(4/6)
568 GCGGG
Ksp632I 300 CTCTTC CTCTTC(1/4)
940 GAAGAG
MflI 332 AGATCT [GA]^GATC[CT]
571 GGATCT
MroI 816 TCCGGA T^CCGGA
1202 TCCGGA
NcoI 164 CCATGG C^CATGG
452 CCATGG
PpuMI 206 AGGACCC [GA]G^G[AT]CC[CT]
770 GGGACCC
RcaI 612 TCATGA T^CATGA
989 TCATGA
SmlI 192 CTCAAG C^T[CT][GA]AG
886 CTCAAG
StuI 246 AGGCCT AGG^CCT
269 AGGCCT
TaqI 605 TCGA T^CGA
1079 TCGA
Tsp45I 463 GTCAC ^GT[GC]AC
1004 GTGAC
TthHB8I 605 TCGA T^CGA
1079 TCGA
XhoII 332 AGATCT [GA]^GATC[CT]
571 GGATCT
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI Asp700I AvaII
BanI BbvI BfaI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI XmnI