Restriction map for
pFN21AB0402
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccII AccIII AclI AflII
Alw44I AocI Aor51HI ApaLI AscI
AspHI AspI AvaI AvaII AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspEI BspHI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KspI MluI
MroI MspA1I MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1071 GGTACC G^GTACC
AccI 1280 GTAGAC GT^[AC][GT]AC
AcyI 1847 GACGCC G[GA]^CG[CT]C
AgeI 676 ACCGGT A^CCGGT
AhdI 649 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 305 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1873 ATTAAT AT^TAAT
AsnI 1873 ATTAAT AT^TAAT
Asp700I 719 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1071 GGTACC G^GTACC
AspEI 649 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1760 GGATCC G^GATCC
BsaBI 1438 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1847 GACGCC G[GA]^CG[CT]C
BsaI 1021 GAGACC GGTCTC(1/5)
BsaWI 676 ACCGGT [AT]^CCGG[AT]
BsmFI 657 GTCCC GGGAC(10/14)
BspDI 703 ATCGAT AT^CGAT
BspLU11I 979 ACATGT A^CATGT
BsrBI 1246 GAGCGG CCGCTC(-3/-3)
BsrFI 676 ACCGGT [GA]^CCGG[CT]
BstBI 584 TTCGAA TT^CGAA
BstXI 1350 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1760 GGATCC [GA]^GATC[CT]
Cfr10I 676 ACCGGT [GA]^CCGG[CT]
ClaI 703 ATCGAT AT^CGAT
DraI 100 TTTAAA TTT^AAA
Eam1105I 649 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1768 GATATC GAT^ATC
EcoT22I 695 ATGCAT ATGCA^T
FauI 1248 GCGGG CCCGC(4/6)
HapII 677 CCGG C^CGG
HgaI 1847 GACGC GACGC(5/10)
Hin1I 1847 GACGCC G[GA]^CG[CT]C
HindIII 862 AAGCTT A^AGCTT
HpaII 677 CCGG C^CGG
KpnI 1071 GGTACC GGTAC^C
MamI 1438 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 599 CAATTG C^AATTG
MflI 1760 GGATCC [GA]^GATC[CT]
MspI 677 CCGG C^CGG
MunI 599 CAATTG C^AATTG
NsiI 695 ATGCAT ATGCA^T
NspI 979 ACATGT [GA]CATG^[CT]
NspV 584 TTCGAA TT^CGAA
PinAI 676 ACCGGT A^CCGGT
PshBI 1873 ATTAAT AT^TAAT
SfuI 584 TTCGAA TT^CGAA
SspI 947 AATATT AAT^ATT
StuI 1141 AGGCCT AGG^CCT
XhoII 1760 GGATCC [GA]^GATC[CT]
XmnI 719 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 171 CCGC CCGC(-3/-1)
1248 GCGG
AflIII 206 ACACGT A^C[GA][CT]GT
979 ACATGT
AlwI 1761 GATCC GGATC(4/5)
1760 GGATC
ApaI 396 GGGCCC GGGCC^C
551 GGGCCC
BbsI 1585 GAAGAC GAAGAC(2/6)
1620 GAAGAC
BfaI 1045 CTAG C^TAG
1651 CTAG
BpuAI 1585 GAAGAC GAAGAC(2/6)
1620 GAAGAC
BseRI 339 GAGGAG GAGGAG(10/8)
835 GAGGAG
BsgI 1232 CTGCAC GTGCAG(16/14)
1604 CTGCAC
BsmI 316 GAATGC GAATGC(1/-1)
488 GCATTC
Bsp1407I 685 TGTACA T^GTACA
768 TGTACA
BspMI 373 ACCTGC ACCTGC(4/8)
1230 ACCTGC
BsrDI 1704 CATTGC GCAATG(2/0)
83 GCAATG
BsrGI 685 TGTACA T^GTACA
768 TGTACA
Cac8I 937 GCTGGC GC[ACGT]^[ACGT]GC
1573 GCAAGC
DraII 395 AGGGCCC [GA]G^G[ACGT]CC[CT]
396 GGGCCCT
EcoO109I 395 AGGGCCC [GA]G^G[ACGT]CC[CT]
396 GGGCCCT
MaeI 1045 CTAG C^TAG
1651 CTAG
MaeII 208 ACGT A^CGT
1344 ACGT
PleI 846 GACTC GAGTC(4/5)
1693 GACTC
SapI 1243 GAAGAGC GCTCTTC(1/4)
1577 GCTCTTC
ScaI 494 AGTACT AGT^ACT
1591 AGTACT
SspBI 685 TGTACA T^GTACA
768 TGTACA
XcmI 782 CCAAGATTTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1365 CCAAAGAGGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI ApoI BalI
BanI BanII BbvI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvaI
MwoI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I