Restriction map for
pFN21AE3303
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccII AccIII AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BbeI BbrPI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BstBI
BstEII BstPI BstUI CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HindIII HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI TaqI
TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1174 GGTACC G^GTACC
AccI 475 GTATAC GT^[AC][GT]AC
AclI 1023 AACGTT AA^CGTT
AcyI 880 GGCGTC G[GA]^CG[CT]C
AocI 526 CCTCAGG CC^T[ACGT]AGG
Asp718I 1174 GGTACC G^GTACC
AspHI 213 GAGCAC G[AT]GC[AT]^C
AspI 1597 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 648 GGATCC G^GATCC
BbvCI 660 GCTGAGG CCTCAGC(-5/-2)
BcgI 351 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2011 CTAG C^TAG
BglII 2036 AGATCT A^GATCT
BsaHI 880 GGCGTC G[GA]^CG[CT]C
BseRI 1478 GAGGAG GAGGAG(10/8)
BsiEI 702 CGACCG CG[GA][CT]^CG
BsiHKAI 213 GAGCAC G[AT]GC[AT]^C
BsmBI 514 GAGACG CGTCTC(1/5)
BsmI 324 GAATGC GAATGC(1/-1)
BspMI 1765 ACCTGC ACCTGC(4/8)
BsrFI 2044 ACCGGC [GA]^CCGG[CT]
BssSI 253 CACGAG CACGAG(-5/-1)
Bst1107I 475 GTATAC GTA^TAC
BstZ17I 475 GTATAC GTA^TAC
Bsu36I 526 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2044 ACCGGC [GA]^CCGG[CT]
Eco81I 526 CCTCAGG CC^T[ACGT]AGG
EcoNI 1297 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1232 GAATTC G^AATTC
HaeII 2184 AGCGCC [GA]GCGC^[CT]
Hin1I 880 GGCGTC G[GA]^CG[CT]C
HincII 610 GTCAAC GT[CT]^[GA]AC
HindII 610 GTCAAC GT[CT]^[GA]AC
KpnI 1174 GGTACC GGTAC^C
MaeI 2011 CTAG C^TAG
NdeI 1074 CATATG CA^TATG
NspI 1868 GCATGT [GA]CATG^[CT]
PflFI 1597 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 787 GACTC GAGTC(4/5)
PpuMI 1601 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1023 AACGTT AA^CGTT
PvuII 401 CAGCTG CAG^CTG
SexAI 1598 ACCAGGT A^CC[AT]GGT
SfcI 724 CTACAG C^T[GA][CT]AG
Tth111I 1597 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 1088 CCCGGG C^[CT]CG[GA]G
1170 CCCGGG
BanII 423 GAGCCC G[GA]GC[CT]^C
1530 GAGCCC
BsgI 903 CTGCAC GTGCAG(16/14)
2057 GTGCAG
BsoBI 1088 CCCGGG C^[CT]CG[GA]G
1170 CCCGGG
BspHI 173 TCATGA T^CATGA
1616 TCATGA
BstAPI 328 GCAGATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
854 GCAACTTCTGC
BstYI 648 GGATCC [GA]^GATC[CT]
2036 AGATCT
DsaI 738 CCATGG C^C[GA][CT]GG
2021 CCATGG
HgaI 1645 GACGC GACGC(5/10)
881 GCGTC
MaeII 970 ACGT A^CGT
1024 ACGT
MflI 648 GGATCC [GA]^GATC[CT]
2036 AGATCT
MseI 104 TTAA T^TAA
2162 TTAA
NcoI 738 CCATGG C^CATGG
2021 CCATGG
PflMI 633 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1883 CCAATCTCTGG
RcaI 173 TCATGA T^CATGA
1616 TCATGA
SapI 2211 GAAGAGC GCTCTTC(1/4)
2238 GAAGAGC
SmaI 1088 CCCGGG CCC^GGG
1170 CCCGGG
SspI 56 AATATT AAT^ATT
65 AATATT
Tru9I 104 TTAA T^TAA
2162 TTAA
Van91I 633 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1883 CCAATCTCTGG
XcmI 755 CCATCCCATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1574 CCAGAGAGGAAGTGG
XhoII 648 GGATCC [GA]^GATC[CT]
2036 AGATCT
XmaCI 1088 CCCGGG C^CCGGG
1170 CCCGGG
XmaI 1088 CCCGGG C^CCGGG
1170 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XmnI