Restriction map for
pFN21AB0460
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AclI AflIII
ApaI AscI AseI AsnI Asp718I
AspI AviII AvrII BbrPI BbvCI
BcgI BclI BglI BlnI BsaAI
BsaBI BsaI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI BstAPI
BstEII BstPI BstUI ClaI CpoI
DraI DraIII EagI EclXI Eco52I
EcoNI EcoO65I FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 1708 TCCGGA T^CCGGA
AflII 411 CTTAAG C^TTAAG
AgeI 1343 ACCGGT A^CCGGT
AhdI 2659 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 178 GTGCAC G^TGCAC
AocI 2007 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2397 AGCGCT AGC^GCT
ApaLI 178 GTGCAC G^TGCAC
Asp700I 1357 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2659 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1667 GGATCC G^GATCC
BbeI 960 GGCGCC GGCGC^C
BciVI 1530 GTATCC GTATCC(6/5)
BfrI 411 CTTAAG C^TTAAG
BglII 500 AGATCT A^GATCT
BlpI 2242 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2242 GCTGAGC GC^T[ACGT]AGC
BseAI 1708 TCCGGA T^CCGGA
BsiEI 947 CGGTCG CG[GA][CT]^CG
BsmI 652 GCATTC GAATGC(1/-1)
BspEI 1708 TCCGGA T^CCGGA
BspHI 1595 TCATGA T^CATGA
BsrDI 1224 CATTGC GCAATG(2/0)
BsrFI 1343 ACCGGT [GA]^CCGG[CT]
Bst1107I 1337 GTATAC GTA^TAC
BstBI 1364 TTCGAA TT^CGAA
BstZ17I 1337 GTATAC GTA^TAC
Bsu36I 2007 CCTGAGG CC^T[ACGT]AGG
CelII 2242 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1343 ACCGGT [GA]^CCGG[CT]
Eam1105I 2659 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2397 AGCGCT AGC^GCT
Eco81I 2007 CCTGAGG CC^T[ACGT]AGG
EcoRI 2338 GAATTC G^AATTC
EcoT22I 1213 ATGCAT ATGCA^T
HincII 204 GTTGAC GT[CT]^[GA]AC
HindII 204 GTTGAC GT[CT]^[GA]AC
HindIII 1423 AAGCTT A^AGCTT
KasI 960 GGCGCC G^GCGCC
MroI 1708 TCCGGA T^CCGGA
NarI 960 GGCGCC GG^CGCC
NsiI 1213 ATGCAT ATGCA^T
NspI 1389 GCATGC [GA]CATG^[CT]
NspV 1364 TTCGAA TT^CGAA
PflMI 1079 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1343 ACCGGT A^CCGGT
RcaI 1595 TCATGA T^CATGA
SapI 1378 GAAGAGC GCTCTTC(1/4)
SfoI 960 GGCGCC GGC^GCC
SfuI 1364 TTCGAA TT^CGAA
SphI 1389 GCATGC GCATG^C
Van91I 1079 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1673 CCACCACAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1357 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 769 GTAGAC GT^[AC][GT]AC
1337 GTATAC
AcyI 960 GGCGCC G[GA]^CG[CT]C
2673 GACGCC
AvaI 127 CTCGAG C^[CT]CG[GA]G
2079 CTCGAG
BalI 2 TGGCCA TGG^CCA
575 TGGCCA
BcnI 561 CCGGG CC^[GC]GG
2074 CCCGG
BpmI 2393 CTCCAG CTGGAG(16/14)
2121 CTGGAG
BsaHI 960 GGCGCC G[GA]^CG[CT]C
2673 GACGCC
BsaWI 1343 ACCGGT [AT]^CCGG[AT]
1708 TCCGGA
BsgI 1861 CTGCAC GTGCAG(16/14)
2202 GTGCAG
BsmBI 280 GAGACG CGTCTC(1/5)
604 GAGACG
BsoBI 127 CTCGAG C^[CT]CG[GA]G
2079 CTCGAG
DrdI 282 GACGGCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2751 GACTCAGATGTC
EcoRV 610 GATATC GAT^ATC
1748 GATATC
FauI 1431 GCGGG CCCGC(4/6)
1485 GCGGG
HaeII 960 GGCGCC [GA]GCGC^[CT]
2397 AGCGCT
HgaI 2179 GACGC GACGC(5/10)
2673 GACGC
Hin1I 960 GGCGCC G[GA]^CG[CT]C
2673 GACGCC
MaeII 1165 ACGT A^CGT
2787 ACGT
MluNI 2 TGGCCA TGG^CCA
575 TGGCCA
MscI 2 TGGCCA TGG^CCA
575 TGGCCA
MseI 412 TTAA T^TAA
1502 TTAA
NciI 561 CCGGG CC^[GC]GG
2074 CCCGG
PaeR7I 127 CTCGAG C^TCGAG
2079 CTCGAG
PpuMI 1537 GGGACCT [GA]G^G[AT]CC[CT]
2221 GGGTCCT
PvuII 2068 CAGCTG CAG^CTG
2520 CAGCTG
StuI 415 AGGCCT AGG^CCT
2416 AGGCCT
Tru9I 412 TTAA T^TAA
1502 TTAA
XhoI 127 CTCGAG C^TCGAG
2079 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII