##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BalI        BanI        BbeI        BbrPI       BbsI        
BcgI        BclI        BfrI        BglI        BglII       
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BstAPI      BstBI       
BstEII      BstPI       BstUI       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          909  GTATAC               GT^[AC][GT]AC
AciI          522  GCGG                 CCGC(-3/-1)
AlwNI        1039  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          824  CCTCAGG              CC^T[ACGT]AGG
AspHI         580  GAGCTC               G[AT]GC[AT]^C
AvrII         804  CCTAGG               C^CTAGG
BamHI         429  GGATCC               G^GATCC
BbvCI         626  CCTCAGC              CCTCAGC(-5/-2)
BlnI          804  CCTAGG               C^CTAGG
BsaI          232  GAGACC               GGTCTC(1/5)
BsiHKAI       580  GAGCTC               G[AT]GC[AT]^C
BsmBI         355  CGTCTC               CGTCTC(1/5)
BspMI         461  ACCTGC               ACCTGC(4/8)
Bst1107I      909  GTATAC               GTA^TAC
BstYI         429  GGATCC               [GA]^GATC[CT]
BstZ17I       909  GTATAC               GTA^TAC
Bsu36I        824  CCTCAGG              CC^T[ACGT]AGG
DpnII         430  GATC                 ^GATC
Eco81I        824  CCTCAGG              CC^T[ACGT]AGG
EcoNI         791  CCTCACGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          522  GCGGG                CCCGC(4/6)
HgaI          515  GCGTC                GACGC(5/10)
HphI          159  TCACC                GGTGA(8/7)
MboI          430  GATC                 ^GATC
MflI          429  GGATCC               [GA]^GATC[CT]
MslI          942  CACCCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        539  CAGCTG               C[AC]G^C[GT]G
NdeII         430  GATC                 ^GATC
PstI          373  CTGCAG               CTGCA^G
PvuII         539  CAGCTG               CAG^CTG
SacI          580  GAGCTC               GAGCT^C
SapI          915  GCTCTTC              GCTCTTC(1/4)
Sau3AI        430  GATC                 ^GATC
SfaNI         489  GATGC                GCATC(5/9)
SfcI          373  CTGCAG               C^T[GA][CT]AG
Tsp509I        14  AATT                 ^AATT
XcmI          891  CCATCCTCCTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         429  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AlwI          430  GATCC                GGATC(4/5)
              429  GGATC               
ApaI          368  GGGCCC               GGGCC^C
              524  GGGCCC              
BciVI         733  GGATAC               GTATCC(6/5)
              746  GGATAC              
BfaI          562  CTAG                 C^TAG
              805  CTAG                
BssSI          22  CTCGTG               CACGAG(-5/-1)
              770  CTCGTG              
BstXI         710  CCAGGTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              941  CCACCCCTGTGG        
Cac8I         186  GCCTGC               GC[ACGT]^[ACGT]GC
              522  GCGGGC              
HaeIII        369  GGCC                 GG^CC
              525  GGCC                
MaeI          562  CTAG                 C^TAG
              805  CTAG                
MwoI           70  GCTCCTCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              134  GCAGACTGAGC         
NlaIII         30  CATG                 CATG^
              121  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AluI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DraII       DrdI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HapII       HinfI       
HpaII       ItaI        Ksp632I     MaeIII      MboII       
MnlI        MspI        MvaI        NciI        NlaIV       
PleI        PpuMI       Sau96I      ScrFI       StyI        
TfiI        TseI        Tsp45I      TspRI