Restriction map for
pFN21AE5086
##################################
# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI ScaI SexAI SfaNI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 111 CGCG CG^CG
AccIII 229 TCCGGA T^CCGGA
AlwI 9 GATCC GGATC(4/5)
AvaI 55 CCCGAG C^[CT]CG[GA]G
BanII 60 GGGCTC G[GA]GC[CT]^C
BbsI 25 GAAGAC GAAGAC(2/6)
BbvI 80 GCTGC GCAGC(8/12)
BmyI 60 GGGCTC G[AGT]GC[ACT]^C
BpuAI 25 GAAGAC GAAGAC(2/6)
BsaJI 56 CCGAGG C^C[ACGT][ACGT]GG
BseAI 229 TCCGGA T^CCGGA
BsiEI 153 CGGCCG CG[GA][CT]^CG
BsoBI 55 CCCGAG C^[CT]CG[GA]G
Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C
BspEI 229 TCCGGA T^CCGGA
BssKI 120 CCAGG ^CC[ACGT]GG
BstNI 120 CCAGG CC^[AT]GG
BstUI 111 CGCG CG^CG
BstXI 161 CCAAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 8 AGATCC [GA]^GATC[CT]
CfoI 47 GCGC GCG^C
EaeI 153 CGGCCG [CT]^GGCC[GA]
EagI 153 CGGCCG C^GGCCG
EclXI 153 CGGCCG C^GGCCG
Eco52I 153 CGGCCG C^GGCCG
EcoRII 120 CCAGG ^CC[AT]GG
FauI 112 GCGGG CCCGC(4/6)
HhaI 47 GCGC GCG^C
HinP1I 47 GCGC G^CGC
HphI 171 GGTGA GGTGA(8/7)
MaeII 197 ACGT A^CGT
MflI 8 AGATCC [GA]^GATC[CT]
MroI 229 TCCGGA T^CCGGA
MspA1I 78 CAGCTG C[AC]G^C[GT]G
MvaI 120 CCAGG CC^[AT]GG
MvnI 111 CGCG CG^CG
NlaIV 61 GGCTCC GG[ACGT]^[ACGT]CC
PstI 88 CTGCAG CTGCA^G
PvuII 78 CAGCTG CAG^CTG
SbfI 87 CCTGCAGG CCTGCA^GG
ScrFI 120 CCAGG CC^[ACGT]GG
SfiI 145 GGCCAGATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 87 CCTGCAGG CCTGCA^GG
TaqI 176 TCGA T^CGA
TseI 80 GCTGC G^C[AT]GC
TthHB8I 176 TCGA T^CGA
XhoII 8 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 79 AGCT AG^CT
140 AGCT
BglI 38 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
146 GCCAGATCGGC
BsaWI 185 ACCGGA [AT]^CCGG[AT]
229 TCCGGA
BstF5I 180 GGATG GGATG(2/0)
209 GGATG
Cac8I 86 GCCTGC GC[ACGT]^[ACGT]GC
112 GCGGGC
Cfr13I 36 GGGCC G^G[ACGT]CC
144 GGGCC
FokI 180 GGATG GGATG(9/13)
209 GGATG
HapII 186 CCGG C^CGG
230 CCGG
HpaII 186 CCGG C^CGG
230 CCGG
MboII 25 GAAGA GAAGA(8/7)
107 TCTTC
MspI 186 CCGG C^CGG
230 CCGG
MwoI 38 GCCGCTTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
146 GCCAGATCGGC
Sau96I 36 GGGCC G^G[ACGT]CC
144 GGGCC
SfcI 18 CTACAG C^T[GA][CT]AG
88 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI BsiYI BslI DpnII Fnu4HI
HaeIII ItaI MboI MnlI NdeII
Sau3AI