Restriction map for
pFN21AE1901
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AspEI AspHI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KspI MaeII
MamI MluI MluNI MroI MscI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1210 GGTACC G^GTACC
AcsI 862 GAATTC [GA]^AATT[CT]
AocI 730 CCTCAGG CC^T[ACGT]AGG
ApoI 862 GAATTC [GA]^AATT[CT]
AseI 156 ATTAAT AT^TAAT
AsnI 156 ATTAAT AT^TAAT
Asp718I 1210 GGTACC G^GTACC
AspI 1022 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 193 GGTCC G^G[AT]CC
BamHI 55 GGATCC G^GATCC
BanII 35 GAGCCC G[GA]GC[CT]^C
BbsI 280 GAAGAC GAAGAC(2/6)
BbvI 835 GCTGC GCAGC(8/12)
BmyI 35 GAGCCC G[AGT]GC[ACT]^C
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaI 1027 GGTCTC GGTCTC(1/5)
BseRI 1115 CTCCTC GAGGAG(10/8)
BsmFI 194 GTCCC GGGAC(10/14)
Bsp1286I 35 GAGCCC G[AGT]GC[ACT]^C
BstXI 700 CCAGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 730 CCTCAGG CC^T[ACGT]AGG
Cfr13I 193 GGTCC G^G[ACGT]CC
DsaI 376 CCGTGG C^C[GA][CT]GG
EarI 625 CTCTTC CTCTTC(1/4)
Eco81I 730 CCTCAGG CC^T[ACGT]AGG
EcoRI 862 GAATTC G^AATTC
EcoRV 1015 GATATC GAT^ATC
EcoT14I 98 CCAAGG C^C[AT][AT]GG
EcoT22I 325 ATGCAT ATGCA^T
HaeIII 249 GGCC GG^CC
HindIII 788 AAGCTT A^AGCTT
KpnI 1210 GGTACC GGTAC^C
Ksp632I 625 CTCTTC CTCTTC(1/4)
MfeI 879 CAATTG C^AATTG
MslI 153 CACATTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 879 CAATTG C^AATTG
NsiI 325 ATGCAT ATGCA^T
NspI 327 GCATGT [GA]CATG^[CT]
PflFI 1022 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 971 GAGTC GAGTC(4/5)
PshBI 156 ATTAAT AT^TAAT
PstI 461 CTGCAG CTGCA^G
Sau96I 193 GGTCC G^G[ACGT]CC
SexAI 1023 ACCAGGT A^CC[AT]GGT
SspI 640 AATATT AAT^ATT
StyI 98 CCAAGG C^C[AT][AT]GG
TseI 835 GCTGC G^C[AT]GC
Tsp45I 985 GTCAC ^GT[GC]AC
Tth111I 1022 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 646 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
679 GAAGCTGTTC
BanI 372 GGCACC G^G[CT][GA]CC
1210 GGTACC
BfaI 386 CTAG C^TAG
1198 CTAG
BpmI 698 CTCCAG CTGGAG(16/14)
593 CTGGAG
BsiYI 96 CCCCAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
586 CCTCTTGCTGG
BslI 96 CCCCAAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
586 CCTCTTGCTGG
BsrBI 3 GAGCGG CCGCTC(-3/-3)
898 GAGCGG
BssKI 777 CCAGG ^CC[ACGT]GG
1024 CCAGG
BstNI 777 CCAGG CC^[AT]GG
1024 CCAGG
BstYI 55 GGATCC [GA]^GATC[CT]
694 GGATCT
DdeI 731 CTCAG C^T[ACGT]AG
1106 CTCAG
EcoRII 777 CCAGG ^CC[AT]GG
1024 CCAGG
MaeI 386 CTAG C^TAG
1198 CTAG
MaeIII 736 GTTAC ^GT[ACGT]AC
985 GTCAC
MflI 55 GGATCC [GA]^GATC[CT]
694 GGATCT
MseI 157 TTAA T^TAA
415 TTAA
MvaI 777 CCAGG CC^[AT]GG
1024 CCAGG
ScrFI 777 CCAGG CC^[ACGT]GG
1024 CCAGG
SfaNI 843 GATGC GCATC(5/9)
670 GCATC
SfcI 113 CTACAG C^T[GA][CT]AG
461 CTGCAG
TfiI 811 GATTC G^A[AT]TC
909 GATTC
Tru9I 157 TTAA T^TAA
415 TTAA
XhoII 55 GGATCC [GA]^GATC[CT]
694 GGATCT
XmnI 646 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
679 GAAGCTGTTC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI AluI AlwI BsaJI
BsgI BsmAI BsrI BstF5I DpnII
Fnu4HI FokI HinfI HphI ItaI
MboI MboII MnlI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Tsp509I
TspRI