Restriction map for
pFN21AE3687
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
Acc65I AccII AccIII AciI AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BcgI BcnI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmFI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I FauI FokI FseI
FspI HaeII HapII HgaI HhaI
HinP1I HpaII KasI KpnI KspI
MamI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI StyI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 189 GACGTC GACGT^C
AccI 1148 GTAGAC GT^[AC][GT]AC
AcyI 189 GACGTC G[GA]^CG[CT]C
AflII 1083 CTTAAG C^TTAAG
AlwNI 120 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 406 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 928 GAGCAC G[AT]GC[AT]^C
BamHI 771 GGATCC G^GATCC
BbvCI 822 CCTCAGC CCTCAGC(-5/-2)
BbvI 660 GCAGC GCAGC(8/12)
BciVI 9 GTATCC GTATCC(6/5)
BfrI 1083 CTTAAG C^TTAAG
BglII 1332 AGATCT A^GATCT
BmyI 928 GAGCAC G[AGT]GC[ACT]^C
BsaHI 189 GACGTC G[GA]^CG[CT]C
BsiHKAI 928 GAGCAC G[AT]GC[AT]^C
BsmBI 187 GAGACG CGTCTC(1/5)
BsmI 1359 GCATTC GAATGC(1/-1)
Bsp1286I 928 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 885 TGTACA T^GTACA
BsrDI 834 CATTGC GCAATG(2/0)
BsrGI 885 TGTACA T^GTACA
EcoRI 275 GAATTC G^AATTC
EcoT22I 1019 ATGCAT ATGCA^T
Fnu4HI 660 GCAGC GC^[ACGT]GC
Hin1I 189 GACGTC G[GA]^CG[CT]C
HindIII 548 AAGCTT A^AGCTT
HpaI 1165 GTTAAC GTT^AAC
ItaI 660 GCAGC GC^[ACGT]GC
MfeI 416 CAATTG C^AATTG
MunI 416 CAATTG C^AATTG
NdeI 664 CATATG CA^TATG
NsiI 1019 ATGCAT ATGCA^T
ScaI 947 AGTACT AGT^ACT
SexAI 251 ACCTGGT A^CC[AT]GGT
SspBI 885 TGTACA T^GTACA
SwaI 1262 ATTTAAAT ATTT^AAAT
TaqI 705 TCGA T^CGA
TseI 660 GCAGC G^C[AT]GC
TthHB8I 705 TCGA T^CGA
XmnI 406 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AseI 1029 ATTAAT AT^TAAT
1422 ATTAAT
AsnI 1029 ATTAAT AT^TAAT
1422 ATTAAT
BbsI 30 GAAGAC GAAGAC(2/6)
988 GAAGAC
BclI 108 TGATCA T^GATCA
855 TGATCA
BpuAI 30 GAAGAC GAAGAC(2/6)
988 GAAGAC
BseRI 894 CTCCTC GAGGAG(10/8)
376 GAGGAG
BsrI 260 CCAGT ACTGG(1/-1)
1438 CCAGT
BssKI 252 CCTGG ^CC[ACGT]GG
924 CCAGG
BstNI 252 CCTGG CC^[AT]GG
924 CCAGG
BstYI 771 GGATCC [GA]^GATC[CT]
1332 AGATCT
DraI 147 TTTAAA TTT^AAA
1263 TTTAAA
EarI 334 GAAGAG CTCTTC(1/4)
766 GAAGAG
EcoRII 252 CCTGG ^CC[AT]GG
924 CCAGG
FbaI 108 TGATCA T^GATCA
855 TGATCA
HincII 358 GTCAAC GT[CT]^[GA]AC
1165 GTTAAC
HindII 358 GTCAAC GT[CT]^[GA]AC
1165 GTTAAC
Ksp632I 334 GAAGAG CTCTTC(1/4)
766 GAAGAG
MaeII 190 ACGT A^CGT
684 ACGT
MflI 771 GGATCC [GA]^GATC[CT]
1332 AGATCT
MspA1I 933 CAGCTG C[AC]G^C[GT]G
1364 CAGCTG
MvaI 252 CCTGG CC^[AT]GG
924 CCAGG
MwoI 570 GCAGAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1079 GCCTCTTAAGC
NlaIV 255 GGTTCC GG[ACGT]^[ACGT]CC
771 GGATCC
PshBI 1029 ATTAAT AT^TAAT
1422 ATTAAT
PvuII 933 CAGCTG CAG^CTG
1364 CAGCTG
ScrFI 252 CCTGG CC^[ACGT]GG
924 CCAGG
SmlI 897 CTCAAG C^T[CT][GA]AG
1083 CTTAAG
SphI 432 GCATGC GCATG^C
1017 GCATGC
XbaI 279 TCTAGA T^CTAGA
1347 TCTAGA
XhoII 771 GGATCC [GA]^GATC[CT]
1332 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BsmAI Cac8I DdeI DpnII
HaeIII HinfI HphI MaeI MaeIII
MboI MboII MnlI MseI MslI
NdeII NlaIII NspI PleI RsaI
Sau3AI SfcI SspI TfiI Tru9I
Tsp45I Tsp509I TspRI