Restriction map for
pFN21AB4048
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI FseI FspI HaeII HhaI
HinP1I HpaI KasI MamI MflI
MluI MroI MslI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1605 GGTACC G^GTACC
AccII 97 CGCG CG^CG
AcyI 494 GACGCC G[GA]^CG[CT]C
AflIII 167 ACATGT A^C[GA][CT]GT
Alw44I 383 GTGCAC G^TGCAC
AocI 1501 CCTAAGG CC^T[ACGT]AGG
ApaLI 383 GTGCAC G^TGCAC
Asp718I 1605 GGTACC G^GTACC
BalI 417 TGGCCA TGG^CCA
BanI 1605 GGTACC G^G[CT][GA]CC
BbsI 749 GTCTTC GAAGAC(2/6)
BclI 1722 TGATCA T^GATCA
BpuAI 749 GTCTTC GAAGAC(2/6)
BsaHI 494 GACGCC G[GA]^CG[CT]C
BsgI 468 CTGCAC GTGCAG(16/14)
BsiEI 38 CGGTCG CG[GA][CT]^CG
Bsp1407I 232 TGTACA T^GTACA
BspLU11I 167 ACATGT A^CATGT
BsrGI 232 TGTACA T^GTACA
BstUI 97 CGCG CG^CG
Bsu36I 1501 CCTAAGG CC^T[ACGT]AGG
DraI 1615 TTTAAA TTT^AAA
Eco81I 1501 CCTAAGG CC^T[ACGT]AGG
EcoRV 103 GATATC GAT^ATC
EcoT22I 622 ATGCAT ATGCA^T
FbaI 1722 TGATCA T^GATCA
HgaI 494 GACGC GACGC(5/10)
Hin1I 494 GACGCC G[GA]^CG[CT]C
HincII 1300 GTTGAC GT[CT]^[GA]AC
HindII 1300 GTTGAC GT[CT]^[GA]AC
KpnI 1605 GGTACC GGTAC^C
KspI 96 CCGCGG CCGC^GG
MaeIII 556 GTCAC ^GT[ACGT]AC
MfeI 560 CAATTG C^AATTG
MluNI 417 TGGCCA TGG^CCA
MscI 417 TGGCCA TGG^CCA
MunI 560 CAATTG C^AATTG
MvnI 97 CGCG CG^CG
NcoI 605 CCATGG C^CATGG
NdeI 1027 CATATG CA^TATG
NsiI 622 ATGCAT ATGCA^T
PflMI 200 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1159 GACTC GAGTC(4/5)
PpuMI 1496 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1371 GAGCTC GAGCT^C
SacII 96 CCGCGG CCGC^GG
SapI 192 GCTCTTC GCTCTTC(1/4)
ScaI 801 AGTACT AGT^ACT
SexAI 71 ACCAGGT A^CC[AT]GGT
SspBI 232 TGTACA T^GTACA
SspI 1624 AATATT AAT^ATT
Tsp45I 556 GTCAC ^GT[GC]AC
Van91I 200 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 797 CCAAAGTACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwI 609 GGATC GGATC(4/5)
681 GGATC
BanII 1310 GGGCTC G[GA]GC[CT]^C
1371 GAGCTC
BcnI 78 CCGGG CC^[GC]GG
771 CCGGG
BfaI 745 CTAG C^TAG
1090 CTAG
BpmI 1691 CTCCAG CTGGAG(16/14)
393 CTGGAG
BseRI 1010 GAGGAG GAGGAG(10/8)
1277 GAGGAG
BstAPI 512 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
576 GCATTCTTTGC
DraII 143 AGGGCCT [GA]G^G[ACGT]CC[CT]
1496 AGGACCC
DsaI 96 CCGCGG C^C[GA][CT]GG
605 CCATGG
EaeI 417 TGGCCA [CT]^GGCC[GA]
1213 CGGCCA
EcoNI 346 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1525 CCTCAAACAGG
EcoO109I 143 AGGGCCT [GA]G^G[ACGT]CC[CT]
1496 AGGACCC
EcoT14I 498 CCAAGG C^C[AT][AT]GG
605 CCATGG
FauI 56 GCGGG CCCGC(4/6)
98 GCGGG
HapII 78 CCGG C^CGG
771 CCGG
HindIII 400 AAGCTT A^AGCTT
731 AAGCTT
HpaII 78 CCGG C^CGG
771 CCGG
MaeI 745 CTAG C^TAG
1090 CTAG
MaeII 333 ACGT A^CGT
1446 ACGT
MspI 78 CCGG C^CGG
771 CCGG
NciI 78 CCGGG CC^[GC]GG
771 CCGGG
PstI 1076 CTGCAG CTGCA^G
1772 CTGCAG
SfcI 1076 CTGCAG C^T[GA][CT]AG
1772 CTGCAG
StyI 498 CCAAGG C^C[AT][AT]GG
605 CCATGG
TaqI 162 TCGA T^CGA
813 TCGA
TthHB8I 162 TCGA T^CGA
813 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbvI BmyI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI