Restriction map for
pFN21AE1273
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BciVI BcnI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BstEII BstPI ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III EcoO65I EcoRI EcoT22I
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 523 GTATAC GT^[AC][GT]AC
AocI 988 CCTGAGG CC^T[ACGT]AGG
BbvCI 1171 GCTGAGG CCTCAGC(-5/-2)
BcgI 1971 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1199 TGATCA T^GATCA
BfaI 670 CTAG C^TAG
BglI 1994 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1390 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1390 GCTGAGC GC^T[ACGT]AGC
BsaI 1915 GAGACC GGTCTC(1/5)
BsiEI 1408 CGGCCG CG[GA][CT]^CG
BsmFI 165 GTCCC GGGAC(10/14)
BsrFI 1406 GCCGGC [GA]^CCGG[CT]
Bst1107I 523 GTATAC GTA^TAC
BstBI 740 TTCGAA TT^CGAA
BstXI 1145 CCAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1554 AGATCC [GA]^GATC[CT]
BstZ17I 523 GTATAC GTA^TAC
Bsu36I 988 CCTGAGG CC^T[ACGT]AGG
CelII 1390 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1406 GCCGGC [GA]^CCGG[CT]
EaeI 1408 CGGCCG [CT]^GGCC[GA]
EagI 1408 CGGCCG C^GGCCG
EclXI 1408 CGGCCG C^GGCCG
Eco52I 1408 CGGCCG C^GGCCG
Eco81I 988 CCTGAGG CC^T[ACGT]AGG
EcoNI 1026 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1465 GATATC GAT^ATC
EcoT14I 1497 CCAAGG C^C[AT][AT]GG
FauI 1281 GCGGG CCCGC(4/6)
FbaI 1199 TGATCA T^GATCA
FseI 1405 GGCCGGCC GGCCGG^CC
HaeII 138 GGCGCT [GA]GCGC^[CT]
HindIII 1295 AAGCTT A^AGCTT
MaeI 670 CTAG C^TAG
MflI 1554 AGATCC [GA]^GATC[CT]
NaeI 1406 GCCGGC GCC^GGC
NgoMIV 1406 GCCGGC G^CCGGC
NspV 740 TTCGAA TT^CGAA
PpuMI 1587 AGGACCC [GA]G^G[AT]CC[CT]
SfuI 740 TTCGAA TT^CGAA
StyI 1497 CCAAGG C^C[AT][AT]GG
XhoII 1554 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 335 GTGCAC G^TGCAC
665 GTGCAC
ApaLI 335 GTGCAC G^TGCAC
665 GTGCAC
Asp700I 259 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
877 GAAGCAGTTC
AvaI 827 CCCGAG C^[CT]CG[GA]G
1592 CCCGAG
AvaII 1485 GGACC G^G[AT]CC
1588 GGACC
BsoBI 827 CCCGAG C^[CT]CG[GA]G
1592 CCCGAG
BssSI 373 CACGAG CACGAG(-5/-1)
1577 CTCGTG
BstAPI 667 GCACTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1308 GCAGAGGCTGC
BstF5I 11 GGATG GGATG(2/0)
1677 GGATG
DraII 1022 AGGCCCT [GA]G^G[ACGT]CC[CT]
1587 AGGACCC
EcoO109I 1022 AGGCCCT [GA]G^G[ACGT]CC[CT]
1587 AGGACCC
FokI 11 GGATG GGATG(9/13)
1677 GGATG
HapII 1407 CCGG C^CGG
1431 CCGG
HgaI 45 GACGC GACGC(5/10)
111 GACGC
HpaII 1407 CCGG C^CGG
1431 CCGG
MseI 298 TTAA T^TAA
449 TTAA
MspI 1407 CCGG C^CGG
1431 CCGG
PleI 684 GACTC GAGTC(4/5)
163 GAGTC
Tru9I 298 TTAA T^TAA
449 TTAA
XmnI 259 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
877 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI BbsI
BbvI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I