Restriction map for
pFN21AE4077
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AclI 895 AACGTT AA^CGTT
AocI 808 CCTGAGG CC^T[ACGT]AGG
AvaI 836 CTCGAG C^[CT]CG[GA]G
BbsI 265 GAAGAC GAAGAC(2/6)
BmyI 165 GTGCCC G[AGT]GC[ACT]^C
BpmI 556 CTCCAG CTGGAG(16/14)
BpuAI 265 GAAGAC GAAGAC(2/6)
BsaI 141 GAGACC GGTCTC(1/5)
BsaJI 534 CCAAGG C^C[ACGT][ACGT]GG
BseRI 431 CTCCTC GAGGAG(10/8)
BsmFI 29 GTCCC GGGAC(10/14)
BsoBI 836 CTCGAG C^[CT]CG[GA]G
Bsp1286I 165 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 780 TGTACA T^GTACA
BspMI 286 ACCTGC ACCTGC(4/8)
BsrDI 355 CATTGC GCAATG(2/0)
BsrGI 780 TGTACA T^GTACA
BssSI 59 CTCGTG CACGAG(-5/-1)
Bsu36I 808 CCTGAGG CC^T[ACGT]AGG
Cac8I 773 GCCAGC GC[ACGT]^[ACGT]GC
Eco81I 808 CCTGAGG CC^T[ACGT]AGG
EcoRI 152 GAATTC G^AATTC
EcoT14I 534 CCAAGG C^C[AT][AT]GG
HaeIII 947 GGCC GG^CC
HpaI 21 GTTAAC GTT^AAC
HphI 284 TCACC GGTGA(8/7)
MaeII 896 ACGT A^CGT
MslI 158 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 33 CATATG CA^TATG
NspI 777 GCATGT [GA]CATG^[CT]
PaeR7I 836 CTCGAG C^TCGAG
PleI 733 GAGTC GAGTC(4/5)
Psp1406I 895 AACGTT AA^CGTT
SfaNI 118 GCATC GCATC(5/9)
SfcI 389 CTATAG C^T[GA][CT]AG
SspBI 780 TGTACA T^GTACA
SspI 849 AATATT AAT^ATT
StyI 534 CCAAGG C^C[AT][AT]GG
TfiI 211 GAATC G^A[AT]TC
XhoI 836 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 55 GATCC GGATC(4/5)
531 GATCC
Asp700I 103 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
547 GAAGCTGTTC
BbvI 690 GCAGC GCAGC(8/12)
693 GCAGC
BglII 474 AGATCT A^GATCT
785 AGATCT
BsmAI 141 GAGAC GTCTC(1/5)
735 GTCTC
BssKI 944 CCTGG ^CC[ACGT]GG
949 CCTGG
BstNI 944 CCTGG CC^[AT]GG
949 CCTGG
DraI 978 TTTAAA TTT^AAA
987 TTTAAA
EarI 16 GAAGAG CTCTTC(1/4)
730 GAAGAG
EcoRII 944 CCTGG ^CC[AT]GG
949 CCTGG
Fnu4HI 690 GCAGC GC^[ACGT]GC
693 GCAGC
HinfI 211 GAATC G^A[ACGT]TC
733 GAGTC
ItaI 690 GCAGC GC^[ACGT]GC
693 GCAGC
Ksp632I 16 GAAGAG CTCTTC(1/4)
730 GAAGAG
MvaI 944 CCTGG CC^[AT]GG
949 CCTGG
MwoI 167 GCCCTAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
232 GCACTAAGGGC
ScrFI 944 CCTGG CC^[ACGT]GG
949 CCTGG
SmlI 160 CTCAAG C^T[CT][GA]AG
836 CTCGAG
TaqI 837 TCGA T^CGA
1030 TCGA
TseI 690 GCAGC G^C[AT]GC
693 GCAGC
Tsp45I 283 GTCAC ^GT[GC]AC
1160 GTCAC
TspRI 466 CACTG CA[GC]TG[ACGT][ACGT]^
710 CACTG
TthHB8I 837 TCGA T^CGA
1030 TCGA
XmnI 103 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
547 GAAGCTGTTC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BsrI BstF5I BstYI DdeI
DpnII FokI HincII HindII MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIII PshBI RsaI Sau3AI
Tru9I Tsp509I XhoII