Restriction map for
pFN21AE9286
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII ItaI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MroI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TseI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 358 CCGC CCGC(-3/-1)
AfaI 615 GTAC GT^AC
Asp700I 92 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 683 CCCGAG C^[CT]CG[GA]G
AvaII 225 GGACC G^G[AT]CC
BalI 663 TGGCCA TGG^CCA
BamHI 294 GGATCC G^GATCC
BbsI 519 GAAGAC GAAGAC(2/6)
BpuAI 519 GAAGAC GAAGAC(2/6)
BseRI 589 GAGGAG GAGGAG(10/8)
BsiYI 216 CCAGACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 216 CCAGACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 116 GAGAC GTCTC(1/5)
BsmFI 758 GGGAC GGGAC(10/14)
BsoBI 683 CCCGAG C^[CT]CG[GA]G
BsrDI 427 GCAATG GCAATG(2/0)
BsrI 523 ACTGG ACTGG(1/-1)
BstYI 294 GGATCC [GA]^GATC[CT]
Cfr13I 225 GGACC G^G[ACGT]CC
EaeI 663 TGGCCA [CT]^GGCC[GA]
HphI 178 GGTGA GGTGA(8/7)
MflI 294 GGATCC [GA]^GATC[CT]
MluNI 663 TGGCCA TGG^CCA
MscI 663 TGGCCA TGG^CCA
PflMI 216 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 615 GTAC GT^AC
Sau96I 225 GGACC G^G[ACGT]CC
StuI 374 AGGCCT AGG^CCT
TaqI 393 TCGA T^CGA
Tsp509I 263 AATT ^AATT
TthHB8I 393 TCGA T^CGA
Van91I 216 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 666 CCATGAGAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 294 GGATCC [GA]^GATC[CT]
XmnI 92 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 295 GATCC GGATC(4/5)
294 GGATC
BpmI 398 CTCCAG CTGGAG(16/14)
524 CTGGAG
BstF5I 8 GGATG GGATG(2/0)
30 GGATG
DpnII 295 GATC ^GATC
743 GATC
EarI 113 GAAGAG CTCTTC(1/4)
532 GAAGAG
FokI 8 GGATG GGATG(9/13)
30 GGATG
HaeIII 375 GGCC GG^CC
664 GGCC
HincII 281 GTCAAC GT[CT]^[GA]AC
538 GTCAAC
HindII 281 GTCAAC GT[CT]^[GA]AC
538 GTCAAC
Ksp632I 113 GAAGAG CTCTTC(1/4)
532 GAAGAG
MboI 295 GATC ^GATC
743 GATC
MseI 500 TTAA T^TAA
692 TTAA
NdeII 295 GATC ^GATC
743 GATC
NlaIV 294 GGATCC GG[ACGT]^[ACGT]CC
679 GGCTCC
PleI 192 GACTC GAGTC(4/5)
49 GAGTC
Sau3AI 295 GATC ^GATC
743 GATC
SfaNI 9 GATGC GCATC(5/9)
571 GATGC
Tru9I 500 TTAA T^TAA
692 TTAA
TspRI 290 CAGTG CA[GC]TG[ACGT][ACGT]^
444 CAGTG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AspHI BanII BfaI BmyI
BsiHKAI Bsp1286I BssKI BstNI DdeI
EcoRII HinfI MaeI MboII MnlI
MslI MvaI NlaIII SacI ScrFI
TfiI